Overview

  • Product name

    Anti-gamma Adducin antibody
    See all gamma Adducin primary antibodies
  • Description

    Rabbit polyclonal to gamma Adducin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human gamma Adducin aa 431-480. The exact sequence is proprietary.
    Database link: Q9UEY8

  • Positive control

    • IHC-P: Human kidney tissue. WB: HepG2 and HEK-293 cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab230354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 79, 75 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
  • Tissue specificity

    Heart only expresses isoform 1.
  • Sequence similarities

    Belongs to the aldolase class II family. Adducin subfamily.
  • Domain

    Comprised of three regions: a N-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
  • Post-translational
    modifications

    Sumoylated.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADD3 antibody
    • ADDG_HUMAN antibody
    • ADDL antibody
    • Adducin 3 (gamma) antibody
    • Adducin 3 antibody
    • Adducin, gamma antibody
    • Adducin-like antibody
    • Adducin-like protein 70 antibody
    • Gamma-adducin antibody
    see all

Images

  • All lanes : Anti-gamma Adducin antibody (ab230354) at 1/500 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell extracts
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) cell extracts
    Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) cell extracts with immunizing peptide

    Developed using the ECL technique.

    Predicted band size: 79, 75 kDa

  • Formalin-fixed, paraffin-embedded human kidney tissue stained for gamma Adducin with ab230354 at a 1:100 dilution in immunohistochemical analysis.

References

ab230354 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab230354.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up