Key features and details
- Rabbit polyclonal to gamma Adducin
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-gamma Adducin antibody
See all gamma Adducin primary antibodies
DescriptionRabbit polyclonal to gamma Adducin
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human gamma Adducin aa 431-480. The exact sequence is proprietary.
Database link: Q9UEY8
- IHC-P: Human kidney tissue. WB: HepG2 and HEK-293 cell extracts.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 79, 75 kDa.|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionMembrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
Tissue specificityHeart only expresses isoform 1.
Sequence similaritiesBelongs to the aldolase class II family. Adducin subfamily.
DomainComprised of three regions: a N-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
Cellular localizationCytoplasm > cytoskeleton. Cell membrane.
- Information by UniProt
- ADD3 antibody
- ADDG_HUMAN antibody
- ADDL antibody
All lanes : Anti-gamma Adducin antibody (ab230354) at 1/500 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell extracts
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) cell extracts
Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) cell extracts with immunizing peptide
Developed using the ECL technique.
Predicted band size: 79, 75 kDa
Formalin-fixed, paraffin-embedded human kidney tissue stained for gamma Adducin with ab230354 at a 1:100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230354 has not yet been referenced specifically in any publications.