Anti-gamma Adducin antibody (ab230354)
Key features and details
- Rabbit polyclonal to gamma Adducin
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-gamma Adducin antibody
See all gamma Adducin primary antibodies -
Description
Rabbit polyclonal to gamma Adducin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human gamma Adducin aa 431-480. The exact sequence is proprietary.
Database link: Q9UEY8 -
Positive control
- IHC-P: Human kidney tissue. WB: HepG2 and HEK-293 cell extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 79, 75 kDa.
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IHC-P |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 79, 75 kDa. |
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. -
Tissue specificity
Heart only expresses isoform 1. -
Sequence similarities
Belongs to the aldolase class II family. Adducin subfamily. -
Domain
Comprised of three regions: a N-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region. -
Post-translational
modificationsSumoylated. -
Cellular localization
Cytoplasm > cytoskeleton. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 120 Human
- Entrez Gene: 27360 Mouse
- Entrez Gene: 25230 Rat
- Omim: 601568 Human
- SwissProt: Q9UEY8 Human
- SwissProt: Q9QYB5 Mouse
- SwissProt: Q62847 Rat
- Unigene: 501012 Human
see all -
Alternative names
- ADD3 antibody
- ADDG_HUMAN antibody
- ADDL antibody
see all
Images
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All lanes : Anti-gamma Adducin antibody (ab230354) at 1/500 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell extracts
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) cell extracts
Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) cell extracts with immunizing peptide
Developed using the ECL technique.
Predicted band size: 79, 75 kDa -
Formalin-fixed, paraffin-embedded human kidney tissue stained for gamma Adducin with ab230354 at a 1:100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab230354 has not yet been referenced specifically in any publications.