Overview

  • Product name

    Anti-gamma Catenin antibody
    See all gamma Catenin primary antibodies
  • Description

    Goat polyclonal to gamma Catenin
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human gamma Catenin aa 730-743 (C terminal).
    Sequence:

    C-GLRPPYPTADHMLA


    (Peptide available as ab23208)

  • Positive control

    • WB: A431 and NIH3T3 cell lysate and rat eye tissue lysate. IHC-P: Human heart tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab11799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 82 kDa).Can be blocked with Human gamma Catenin peptide (ab23208).

Target

  • Function

    Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
  • Involvement in disease

    Defects in JUP are the cause of Naxos disease (NXD) [MIM:601214]. NXD is an autosomal recessive disorder combining diffuse non-epidermolytic palmoplantar keratoderma with arrhythmogenic right ventricular dysplasia/cardiomyopathy and woolly hair.
    Defects in JUP are the cause of familial arrhythmogenic right ventricular dysplasia type 12 (ARVD12) [MIM:611528]; also called arrhythmogenic right ventricular cardiomyopathy 12 (ARVC12). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.
  • Sequence similarities

    Belongs to the beta-catenin family.
    Contains 9 ARM repeats.
  • Cellular localization

    Cell junction > adherens junction. Cell junction > desmosome. Cytoplasm > cytoskeleton. Membrane. Cytoplasmic in a soluble and membrane-associated form.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARVD 12 antibody
    • ARVD12 antibody
    • Catenin (cadherin associated protein), gamma 80kDa antibody
    • catenin (cadherin-associated protein) gamma (80kD) antibody
    • Catenin gamma antibody
    • CTNNG antibody
    • Desmoplakin 3 antibody
    • Desmoplakin III antibody
    • Desmoplakin-3 antibody
    • Desmoplakin3 antibody
    • DesmoplakinIII antibody
    • DP 3 antibody
    • DP III antibody
    • DP3 antibody
    • DPIII antibody
    • gamma catenin antibody
    • Junction plakoglobin antibody
    • JUP antibody
    • OTTHUMP00000164732 antibody
    • OTTHUMP00000164735 antibody
    • OTTHUMP00000164738 antibody
    • PDGB antibody
    • PKGB antibody
    • PLAK_HUMAN antibody
    • PLAKOGLOBIN antibody
    see all

Images

  • All lanes : Anti-gamma Catenin antibody (ab11799) at 0.3 µg/ml

    Lane 1 : A431 cell lysate in RIPA buffer
    Lane 2 : NIH3T3 cell lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 82 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?



    Primary incubation for 1 hour. Detected using chemiluminescence.

  • Anti-gamma Catenin antibody (ab11799) at 1 µg/ml + Rat eye tissue lysate in RIPA buffer at 35 µg

    Predicted band size: 82 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?



    Primary incubation for 1 hour. Detected using chemiluminescence.

References

This product has been referenced in:

  • Shair KH  et al. EBV latent membrane protein 1 effects on plakoglobin, cell growth, and migration. Cancer Res 68:6997-7005 (2008). WB ; Human . Read more (PubMed: 18757414) »
  • Mainou BA  et al. Unique signaling properties of CTAR1 in LMP1-mediated transformation. J Virol 81:9680-92 (2007). WB ; Rat . Read more (PubMed: 17626074) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (Nasopharyngeal carcinoma cell line)
Loading amount
10 µg
Specification
Nasopharyngeal carcinoma cell line
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jun 22 2010

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