- Datasheet
- Specific References (4)
- Protocols
Overview
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Product nameAnti-gamma Catenin antibody
See all gamma Catenin primary antibodies -
DescriptionRabbit polyclonal to gamma Catenin
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
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Positive control
- Breast carcinoma
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS, BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab15153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 82 kDa. | |
| IP | Use at an assay dependent concentration. | |
| IHC-P | 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 1 µg/ml. |
Target
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FunctionCommon junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
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Involvement in diseaseDefects in JUP are the cause of Naxos disease (NXD) [MIM:601214]. NXD is an autosomal recessive disorder combining diffuse non-epidermolytic palmoplantar keratoderma with arrhythmogenic right ventricular dysplasia/cardiomyopathy and woolly hair.
Defects in JUP are the cause of familial arrhythmogenic right ventricular dysplasia type 12 (ARVD12) [MIM:611528]; also called arrhythmogenic right ventricular cardiomyopathy 12 (ARVC12). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall. -
Sequence similaritiesBelongs to the beta-catenin family.
Contains 9 ARM repeats. -
Cellular localizationCell junction > adherens junction. Cell junction > desmosome. Cytoplasm > cytoskeleton. Membrane. Cytoplasmic in a soluble and membrane-associated form.
- Information by UniProt
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Database links
- Entrez Gene: 3728 Human
- Entrez Gene: 16480 Mouse
- Entrez Gene: 81679 Rat
- Omim: 173325 Human
- SwissProt: P14923 Human
- SwissProt: Q02257 Mouse
- SwissProt: Q6P0K8 Rat
- Unigene: 514174 Human
see all -
Alternative names
- ARVD 12 antibody
- ARVD12 antibody
- Catenin (cadherin associated protein), gamma 80kDa antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody (ab15153)Human breast carcinoma stained with anti gamma catenin antibody ab15153 (1/100 for 10 min at RT). Gamma catenin staining is seen on the cell membrane and in the cytoplasm. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody (ab15153)This image is courtesy of an anonymous Abreviewab15153 staining gamma Catenin in normal human skin tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 10% serum for 1 hour at 2°C; antigen retrival was by heat mediation in EDTA (pH9). The sample was incubated with primary antibody (1/100 in TBS + 1% BSA) at 21°C for 1 hour. An undiluted HRP-conjugated Goat polyclonal to mouse IgG was used as secondary antibody. -
Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody (ab15153)ICC/IF image of ab15153 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15153, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References
This product has been referenced in:
- Boyden LM et al. Dominant de novo DSP mutations cause erythrokeratodermia-cardiomyopathy syndrome. Hum Mol Genet 25:348-57 (2016). Read more (PubMed: 26604139) »
- Guo Z et al. E-cadherin interactome complexity and robustness resolved by quantitative proteomics. Sci Signal 7:rs7 (2014). Read more (PubMed: 25468996) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"