Name: Anti-gamma Catenin antibody [EPR17310]
Brand: RabMAb
SKU: ab184919

Product name

Anti-gamma Catenin antibody [EPR17310]
See all gamma Catenin primary antibodies
Description

Rabbit monoclonal [EPR17310] to gamma Catenin
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Species reactivity

Reacts with: Mouse, Rat, Dog, Human
Immunogen

Recombinant fragment within Human gamma Catenin aa 1-150. The exact sequence is proprietary.
Database link: P14923

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Positive control
- WB: HeLa, A431 and MDCK whole cell lysates; Mouse skin, rat skin, Human fetal heart, fetal kidney and fetal skin lysates. IHC-P: Human skin and prostatic hyperplasia, Mouse stomach and Rat skin tissues. ICC/IF: HeLa and MDCKcells.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17310
Isotype

IgG
Research areas

Alternative Versions
- Anti-gamma Catenin antibody [EPR17310] (Alexa Fluor® 488) (ab237236)
- Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit (ab102918)
- APC Conjugation Kit (ab201807)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human gamma Catenin protein (ab114407)

Our Abpromise guarantee covers the use of ab184919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
IHC-P		1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF		1/250.
Flow Cyt		1/1000.

Function

Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
Involvement in disease

Defects in JUP are the cause of Naxos disease (NXD) [MIM:601214]. NXD is an autosomal recessive disorder combining diffuse non-epidermolytic palmoplantar keratoderma with arrhythmogenic right ventricular dysplasia/cardiomyopathy and woolly hair.
Defects in JUP are the cause of familial arrhythmogenic right ventricular dysplasia type 12 (ARVD12) [MIM:611528]; also called arrhythmogenic right ventricular cardiomyopathy 12 (ARVC12). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.
Sequence similarities

Belongs to the beta-catenin family.
Contains 9 ARM repeats.
Cellular localization

Cell junction > adherens junction. Cell junction > desmosome. Cytoplasm > cytoskeleton. Membrane. Cytoplasmic in a soluble and membrane-associated form.
Target information above from: UniProt accession P14923 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 3728 Human
- Entrez Gene: 16480 Mouse
- Entrez Gene: 81679 Rat
- Omim: 173325 Human
- SwissProt: P14923 Human
- SwissProt: Q02257 Mouse
- SwissProt: Q6P0K8 Rat
- Unigene: 514174 Human
- Unigene: 299774 Mouse
- Unigene: 11255 Rat
see all
Alternative names
- ARVD 12 antibody
- ARVD12 antibody
- Catenin (cadherin associated protein), gamma 80kDa antibody
- catenin (cadherin-associated protein) gamma (80kD) antibody
- Catenin gamma antibody
- CTNNG antibody
- Desmoplakin 3 antibody
- Desmoplakin III antibody
- Desmoplakin-3 antibody
- Desmoplakin3 antibody
- DesmoplakinIII antibody
- DP 3 antibody
- DP III antibody
- DP3 antibody
- DPIII antibody
- gamma catenin antibody
- Junction plakoglobin antibody
- JUP antibody
- OTTHUMP00000164732 antibody
- OTTHUMP00000164735 antibody
- OTTHUMP00000164738 antibody
- PDGB antibody
- PKGB antibody
- PLAK_HUMAN antibody
- PLAKOGLOBIN antibody
see all

Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)

All lanes : Anti-gamma Catenin antibody [EPR17310] (ab184919) at 1/1000 dilution

Lane 1 : Human fetal heart lysates
Lane 2 : Human fetal kidney lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 82 kDa
Observed band size: 82 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time = 15 seconds
Flow Cytometry - Anti-gamma Catenin antibody [EPR17310] (ab184919)

Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) labelling gamma Catenin with purified ab184919 at 1/1000 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] (ab184919)

Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling gamma Catenin with ab184919 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and membrane staining on epithelial cells of Human prostate is observed; and smooth muscle cells are also positively stained. Counterstained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] (ab184919)

Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling gamma Catenin with ab184919 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and membrane staining on epithelial cells of Human skin is observed. Counterstained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] (ab184919)

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling gamma Catenin with ab184919 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and membrane staining on epithelial cells of mouse stomach is observed. Counterstained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] (ab184919)

Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling gamma Catenin with ab184919 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Membrane and cytoplasmic staining on the epithelial cells of rat skin is observed; and smooth muscle cells are also positively stained. Counterstained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [EPR17310] (ab184919)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling gamma Catenin with ab184919 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab184919 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [EPR17310] (ab184919)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDCK (Canine kidney cell line) cells labeling gamma Catenin with ab184919 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing membranous staining on MDCK cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab184919 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)

All lanes : Anti-gamma Catenin antibody [EPR17310] (ab184919) at 1/10000 dilution

Lane 1 : Human fetal skin lysates
Lane 2 : A431 (Human epidermoid carcinoma) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 82 kDa
Observed band size: 82 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time = 3 seconds
Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)

All lanes : Anti-gamma Catenin antibody [EPR17310] (ab184919) at 1/1000 dilution

Lane 1 : Hela (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
Lane 2 : Mouse skin lysates
Lane 3 : Rat skin lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 82 kDa
Observed band size: 82 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time = 5 seconds
Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)

Anti-gamma Catenin antibody [EPR17310] (ab184919) at 1/1000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 20 µg

Secondary
Mouse monoclonal to cardiac Troponin I (ab1000) (Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated)

Predicted band size: 82 kDa
Observed band size: 82 kDa

Blocking and diluting buffer and concentration = 5% NFDM/TBST
Exposure time = 3 minutes
Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)

Anti-gamma Catenin antibody [EPR17310] (ab184919) at 1/100000 dilution + MDCK (Canine kidney cell line) whole cell lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 82 kDa
Observed band size: 82 kDa

Exposure time: 1 minute

Blocking and Diluting buffer and concentration: 5% NFDM/TBST

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Datasheet

This product has been referenced in:

Login FH et al. Aquaporins differentially regulate cell-cell adhesion in MDCK cells. FASEB J 33:6980-6994 (2019). Read more (PubMed: 30840830) »
Lu X et al. Effect and mechanism of the aß2-GP I/rhß2-GP I complex on JEG-3 cell proliferation, migration and invasion. Mol Med Rep 17:7505-7512 (2018). Read more (PubMed: 29620217) »

See all 2 Publications for this product

Publishing research using ab184919? Please let us know so that we can cite the reference in this datasheet.

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Recombinant Anti-gamma Catenin antibody [EPR17310] (ab184919)

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