Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-gamma Catenin antibody [EPR17310] (ab184919)

Reviews (1) Submit a question References (7)
Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)
  • Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)
  • Flow Cytometry (Intracellular) - Anti-gamma Catenin antibody [EPR17310] (ab184919)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] (ab184919)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] (ab184919)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] (ab184919)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] (ab184919)
  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [EPR17310] (ab184919)
  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [EPR17310] (ab184919)
  • Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)
  • Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)
  • Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)
  • Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)
  • Anti-gamma Catenin antibody [EPR17310] (ab184919)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17310] to gamma Catenin
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Mouse, Rat, Dog, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free

Overview

  • Product name

    Anti-gamma Catenin antibody [EPR17310]
    See all gamma Catenin primary antibodies

  • Description

    Rabbit monoclonal [EPR17310] to gamma Catenin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Mouse, Rat, Dog, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T, HeLa, A431 and MDCK whole cell lysates; Mouse skin, rat skin, Human fetal heart, fetal kidney and fetal skin lysates. IHC-P: Human skin and prostatic hyperplasia, Mouse stomach and Rat skin tissues. ICC/IF: HeLa and MDCK cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17310

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab184919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
IHC-P (1)
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/250.
Flow Cyt (Intra)
1/1000.
Notes
WB
1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/250.
Flow Cyt (Intra)
1/1000.

Target

  • Function

    Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.

  • Involvement in disease

    Defects in JUP are the cause of Naxos disease (NXD) [MIM:601214]. NXD is an autosomal recessive disorder combining diffuse non-epidermolytic palmoplantar keratoderma with arrhythmogenic right ventricular dysplasia/cardiomyopathy and woolly hair.
    Defects in JUP are the cause of familial arrhythmogenic right ventricular dysplasia type 12 (ARVD12) [MIM:611528]; also called arrhythmogenic right ventricular cardiomyopathy 12 (ARVC12). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.

  • Sequence similarities

    Belongs to the beta-catenin family.
    Contains 9 ARM repeats.

  • Cellular localization

    Cell junction > adherens junction. Cell junction > desmosome. Cytoplasm > cytoskeleton. Membrane. Cytoplasmic in a soluble and membrane-associated form.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ARVD 12 antibody
    • ARVD12 antibody
    • Catenin (cadherin associated protein), gamma 80kDa antibody
    • catenin (cadherin-associated protein) gamma (80kD) antibody
    • Catenin gamma antibody
    • CTNNG antibody
    • Desmoplakin 3 antibody
    • Desmoplakin III antibody
    • Desmoplakin-3 antibody
    • Desmoplakin3 antibody
    • DesmoplakinIII antibody
    • DP 3 antibody
    • DP III antibody
    • DP3 antibody
    • DPIII antibody
    • gamma catenin antibody
    • Junction plakoglobin antibody
    • JUP antibody
    • OTTHUMP00000164732 antibody
    • OTTHUMP00000164735 antibody
    • OTTHUMP00000164738 antibody
    • PDGB antibody
    • PKGB antibody
    • PLAK_HUMAN antibody
    • PLAKOGLOBIN antibody
    see all

Images

  • Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)
    Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)
    All lanes : Anti-gamma Catenin antibody [EPR17310] (ab184919) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : JUZ knockout HEK-293T cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : THP-1 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 82 kDa
    Observed band size: 82 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab184919 observed at 82 kDa. Red - loading control, ab8245 observed at 37 kDa.

    ab184919 Anti-gamma Catenin antibody [EPR17310] was shown to specifically react with gamma Catenin in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266272 (knockout cell lysate ab257269) was used. Wild-type and gamma Catenin knockout samples were subjected to SDS-PAGE. ab184919 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)
    Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)
    All lanes : Anti-gamma Catenin antibody [EPR17310] (ab184919) at 1/1000 dilution

    Lane 1 : Human fetal heart lysates
    Lane 2 : Human fetal kidney lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 82 kDa
    Observed band size: 82 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time = 15 seconds

  • Flow Cytometry (Intracellular) - Anti-gamma Catenin antibody [EPR17310] (ab184919)
    Flow Cytometry (Intracellular) - Anti-gamma Catenin antibody [EPR17310] (ab184919)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) labelling gamma Catenin with purified ab184919 at 1/1000 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies. 

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] (ab184919)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] (ab184919)

    Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling gamma Catenin with ab184919 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and membrane staining on epithelial cells of Human prostate is observed; and smooth muscle cells are also positively stained. Counterstained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] (ab184919)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] (ab184919)

    Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling gamma Catenin with ab184919 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and membrane staining on epithelial cells of Human skin is observed. Counterstained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] (ab184919)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] (ab184919)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling gamma Catenin with ab184919 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and membrane staining on epithelial cells of mouse stomach is observed. Counterstained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] (ab184919)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] (ab184919)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling gamma Catenin with ab184919 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Membrane and cytoplasmic staining on the epithelial cells of rat skin is observed; and smooth muscle cells are also positively stained. Counterstained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [EPR17310] (ab184919)
    Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [EPR17310] (ab184919)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling gamma Catenin with ab184919 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab184919 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [EPR17310] (ab184919)
    Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [EPR17310] (ab184919)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDCK (Canine kidney cell line) cells labeling gamma Catenin with ab184919 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing membranous staining on MDCK cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab184919 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

     

  • Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)
    Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)
    All lanes : Anti-gamma Catenin antibody [EPR17310] (ab184919) at 1/10000 dilution

    Lane 1 : Human fetal skin lysates
    Lane 2 : A431 (Human epidermoid carcinoma) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 82 kDa
    Observed band size: 82 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time = 3 seconds

  • Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)
    Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)
    All lanes : Anti-gamma Catenin antibody [EPR17310] (ab184919) at 1/1000 dilution

    Lane 1 : Hela (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
    Lane 2 : Mouse skin lysates
    Lane 3 : Rat skin lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 82 kDa
    Observed band size: 82 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time = 5 seconds

  • Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)
    Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)
    Anti-gamma Catenin antibody [EPR17310] (ab184919) at 1/1000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 20 µg

    Secondary
    Mouse monoclonal to cardiac Troponin I (ab1000) (Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated)

    Predicted band size: 82 kDa
    Observed band size: 82 kDa



    Blocking and diluting buffer and concentration = 5% NFDM/TBST

    Exposure time = 3 minutes

  • Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)
    Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)
    Anti-gamma Catenin antibody [EPR17310] (ab184919) at 1/100000 dilution + MDCK (Canine kidney cell line) whole cell lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 82 kDa
    Observed band size: 82 kDa


    Exposure time: 1 minute


    Blocking and Diluting buffer and concentration: 5% NFDM/TBST

  • Anti-gamma Catenin antibody [EPR17310] (ab184919)
    Anti-gamma Catenin antibody [EPR17310] (ab184919)

References (7)

Publishing research using ab184919? Please let us know so that we can cite the reference in this datasheet.

ab184919 has been referenced in 7 publications.

  • Sheng H  et al. Combined Pharmacotherapy with Alendronate and Desferoxamine Regulate the Bone Resorption and Bone Regeneration for Preventing Glucocorticoids-Induced Osteonecrosis of the Femoral Head. Biomed Res Int 2020:3120458 (2020). PubMed: 33029500
  • Badu-Nkansah KA & Lechler T Proteomic analysis of desmosomes reveals novel components required for epidermal integrity. Mol Biol Cell 31:1140-1153 (2020). PubMed: 32238101
  • Xiang J  et al. lncRNA SNHG1 attenuates osteogenic differentiation via the miR-101/DKK1 axis in bone marrow mesenchymal stem cells. Mol Med Rep 22:3715-3722 (2020). PubMed: 32901867
  • Login FH  et al. Aquaporins differentially regulate cell-cell adhesion in MDCK cells. FASEB J 33:6980-6994 (2019). PubMed: 30840830
  • Civciristov S  et al. Ligand-dependent spatiotemporal signaling profiles of the µ-opioid receptor are controlled by distinct protein-interaction networks. J Biol Chem 294:16198-16213 (2019). PubMed: 31515267
  • Tang X  et al. ?-catenin alleviates cardiac fibrosis through inhibiting phosphorylation of GSK-3ß. J Biomed Res 0:1-9 (2019). PubMed: 31741464
  • Lu X  et al. Effect and mechanism of the aß2-GP I/rhß2-GP I complex on JEG-3 cell proliferation, migration and invasion. Mol Med Rep 17:7505-7512 (2018). PubMed: 29620217

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-gamma Catenin antibody [EPR17310]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skeletal muscle)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Leica Bond ER-2
Permeabilization
Yes - Tween-20
Specification
skeletal muscle
Blocking step
Leica Power Vision blocking as blocking agent for 40 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Dec 21 2020

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com