Name: Anti-gamma Catenin antibody [EPR17310]
Brand: RabMAb
SKU: ab184919
Rating: 4 (1 reviews)

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Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17310] to gamma Catenin
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
Knockout validated
Reacts with: Mouse, Rat, Dog, Human

Alexa Fluor® 488 Carrier Free

Product name

Anti-gamma Catenin antibody [EPR17310]
See all gamma Catenin primary antibodies
Description

Rabbit monoclonal [EPR17310] to gamma Catenin
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
Species reactivity

Reacts with: Mouse, Rat, Dog, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293T, HeLa, A431 and MDCK whole cell lysates; Mouse skin, rat skin, Human fetal heart, fetal kidney and fetal skin lysates. IHC-P: Human skin and prostatic hyperplasia, Mouse stomach and Rat skin tissues. ICC/IF: HeLa and MDCK cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17310
Isotype

IgG
Research areas

Alternative Versions
- Alexa Fluor® 488 Anti-gamma Catenin antibody [EPR17310] (ab237236)
- Anti-gamma Catenin antibody [EPR17310] - BSA and Azide free (ab238447)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human JUP (gamma Catenin) knockout HEK-293T cell line (ab266272)
KO cell lysates
- Human JUP (gamma Catenin) knockout HEK-293T cell lysate (ab257269)
Recombinant Protein
- Recombinant Human gamma Catenin protein (ab114407)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab184919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
IHC-P	(1)	1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF		1/250.
Flow Cyt (Intra)		1/1000.

Notes
WB 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/250.
Flow Cyt (Intra) 1/1000.

Function

Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
Involvement in disease

Defects in JUP are the cause of Naxos disease (NXD) [MIM:601214]. NXD is an autosomal recessive disorder combining diffuse non-epidermolytic palmoplantar keratoderma with arrhythmogenic right ventricular dysplasia/cardiomyopathy and woolly hair.
Defects in JUP are the cause of familial arrhythmogenic right ventricular dysplasia type 12 (ARVD12) [MIM:611528]; also called arrhythmogenic right ventricular cardiomyopathy 12 (ARVC12). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.
Sequence similarities

Belongs to the beta-catenin family.
Contains 9 ARM repeats.
Cellular localization

Cell junction > adherens junction. Cell junction > desmosome. Cytoplasm > cytoskeleton. Membrane. Cytoplasmic in a soluble and membrane-associated form.
Target information above from: UniProt accession P14923 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 3728 Human
- Entrez Gene: 16480 Mouse
- Entrez Gene: 81679 Rat
- Omim: 173325 Human
- SwissProt: P14923 Human
- SwissProt: Q02257 Mouse
- SwissProt: Q6P0K8 Rat
- Unigene: 514174 Human
- Unigene: 299774 Mouse
- Unigene: 11255 Rat
see all
Alternative names
- ARVD 12 antibody
- ARVD12 antibody
- Catenin (cadherin associated protein), gamma 80kDa antibody
- catenin (cadherin-associated protein) gamma (80kD) antibody
- Catenin gamma antibody
- CTNNG antibody
- Desmoplakin 3 antibody
- Desmoplakin III antibody
- Desmoplakin-3 antibody
- Desmoplakin3 antibody
- DesmoplakinIII antibody
- DP 3 antibody
- DP III antibody
- DP3 antibody
- DPIII antibody
- gamma catenin antibody
- Junction plakoglobin antibody
- JUP antibody
- OTTHUMP00000164732 antibody
- OTTHUMP00000164735 antibody
- OTTHUMP00000164738 antibody
- PDGB antibody
- PKGB antibody
- PLAK_HUMAN antibody
- PLAKOGLOBIN antibody
see all

Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)

All lanes : Anti-gamma Catenin antibody [EPR17310] (ab184919) at 1/1000 dilution

Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : JUZ knockout HEK-293T cell lysate
Lane 3 : A431 cell lysate
Lane 4 : THP-1 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 82 kDa
Observed band size: 82 kDa

Lanes 1-4: Merged signal (red and green). Green - ab184919 observed at 82 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab184919 Anti-gamma Catenin antibody [EPR17310] was shown to specifically react with gamma Catenin in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266272 (knockout cell lysate ab257269) was used. Wild-type and gamma Catenin knockout samples were subjected to SDS-PAGE. ab184919 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)

All lanes : Anti-gamma Catenin antibody [EPR17310] (ab184919) at 1/1000 dilution

Lane 1 : Human fetal heart lysates
Lane 2 : Human fetal kidney lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 82 kDa
Observed band size: 82 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time = 15 seconds
Flow Cytometry (Intracellular) - Anti-gamma Catenin antibody [EPR17310] (ab184919)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) labelling gamma Catenin with purified ab184919 at 1/1000 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor^® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] (ab184919)

Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling gamma Catenin with ab184919 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and membrane staining on epithelial cells of Human prostate is observed; and smooth muscle cells are also positively stained. Counterstained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] (ab184919)

Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling gamma Catenin with ab184919 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and membrane staining on epithelial cells of Human skin is observed. Counterstained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] (ab184919)

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling gamma Catenin with ab184919 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and membrane staining on epithelial cells of mouse stomach is observed. Counterstained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310] (ab184919)

Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling gamma Catenin with ab184919 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Membrane and cytoplasmic staining on the epithelial cells of rat skin is observed; and smooth muscle cells are also positively stained. Counterstained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [EPR17310] (ab184919)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling gamma Catenin with ab184919 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab184919 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [EPR17310] (ab184919)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDCK (Canine kidney cell line) cells labeling gamma Catenin with ab184919 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing membranous staining on MDCK cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab184919 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)

All lanes : Anti-gamma Catenin antibody [EPR17310] (ab184919) at 1/10000 dilution

Lane 1 : Human fetal skin lysates
Lane 2 : A431 (Human epidermoid carcinoma) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 82 kDa
Observed band size: 82 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time = 3 seconds
Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)

All lanes : Anti-gamma Catenin antibody [EPR17310] (ab184919) at 1/1000 dilution

Lane 1 : Hela (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
Lane 2 : Mouse skin lysates
Lane 3 : Rat skin lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 82 kDa
Observed band size: 82 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time = 5 seconds
Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)

Anti-gamma Catenin antibody [EPR17310] (ab184919) at 1/1000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 20 µg

Secondary
Mouse monoclonal to cardiac Troponin I (ab1000) (Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated)

Predicted band size: 82 kDa
Observed band size: 82 kDa

Blocking and diluting buffer and concentration = 5% NFDM/TBST
Exposure time = 3 minutes
Western blot - Anti-gamma Catenin antibody [EPR17310] (ab184919)

Anti-gamma Catenin antibody [EPR17310] (ab184919) at 1/100000 dilution + MDCK (Canine kidney cell line) whole cell lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 82 kDa
Observed band size: 82 kDa

Exposure time: 1 minute

Blocking and Diluting buffer and concentration: 5% NFDM/TBST
Anti-gamma Catenin antibody [EPR17310] (ab184919)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Datasheet download

Download

Publishing research using ab184919? Please let us know so that we can cite the reference in this datasheet.

ab184919 has been referenced in 7 publications.

Sheng H et al. Combined Pharmacotherapy with Alendronate and Desferoxamine Regulate the Bone Resorption and Bone Regeneration for Preventing Glucocorticoids-Induced Osteonecrosis of the Femoral Head. Biomed Res Int 2020:3120458 (2020). PubMed: 33029500
Badu-Nkansah KA & Lechler T Proteomic analysis of desmosomes reveals novel components required for epidermal integrity. Mol Biol Cell 31:1140-1153 (2020). PubMed: 32238101
Xiang J et al. lncRNA SNHG1 attenuates osteogenic differentiation via the miR-101/DKK1 axis in bone marrow mesenchymal stem cells. Mol Med Rep 22:3715-3722 (2020). PubMed: 32901867
Login FH et al. Aquaporins differentially regulate cell-cell adhesion in MDCK cells. FASEB J 33:6980-6994 (2019). PubMed: 30840830
Civciristov S et al. Ligand-dependent spatiotemporal signaling profiles of the µ-opioid receptor are controlled by distinct protein-interaction networks. J Biol Chem 294:16198-16213 (2019). PubMed: 31515267
Tang X et al. ?-catenin alleviates cardiac fibrosis through inhibiting phosphorylation of GSK-3ß. J Biomed Res 0:1-9 (2019). PubMed: 31741464
Lu X et al. Effect and mechanism of the aß2-GP I/rhß2-GP I complex on JEG-3 cell proliferation, migration and invasion. Mol Med Rep 17:7505-7512 (2018). PubMed: 29620217

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Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (skeletal muscle)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Leica Bond ER-2

Permeabilization

Yes - Tween-20

Specification

skeletal muscle

Blocking step

Leica Power Vision blocking as blocking agent for 40 minute(s) · Concentration: 100% · Temperature: RT°C

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Dec 21 2020

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Conjugation kits

Isotype control

KO cell lines

KO cell lysates

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Involvement in disease

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Datasheet download

Certificate of Compliance

References (7)

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-gamma Catenin antibody [EPR17310]