Anti-gamma Catenin (phospho Y550) antibody (ab29041)
Key features and details
- Rabbit polyclonal to gamma Catenin (phospho Y550)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-gamma Catenin (phospho Y550) antibody
See all gamma Catenin primary antibodies -
Description
Rabbit polyclonal to gamma Catenin (phospho Y550) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human gamma Catenin (phospho Y550) conjugated to keyhole limpet haemocyanin.
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General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab29041 was cross adsorbed to both phospho tyrosine coupled to agarose and to dephosphorylated gamma Catenin (Tyr550) peptide before immunogen affinity purification (without carrier protein). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab29041 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 80 kDa.
In 5% non fat milk, PBS, 0.04% Tween 20 for 1hour at room temperature. |
Notes |
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WB
1/1000. Predicted molecular weight: 80 kDa. In 5% non fat milk, PBS, 0.04% Tween 20 for 1hour at room temperature. |
Target
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Function
Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton. -
Involvement in disease
Defects in JUP are the cause of Naxos disease (NXD) [MIM:601214]. NXD is an autosomal recessive disorder combining diffuse non-epidermolytic palmoplantar keratoderma with arrhythmogenic right ventricular dysplasia/cardiomyopathy and woolly hair.
Defects in JUP are the cause of familial arrhythmogenic right ventricular dysplasia type 12 (ARVD12) [MIM:611528]; also called arrhythmogenic right ventricular cardiomyopathy 12 (ARVC12). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall. -
Sequence similarities
Belongs to the beta-catenin family.
Contains 9 ARM repeats. -
Cellular localization
Cell junction > adherens junction. Cell junction > desmosome. Cytoplasm > cytoskeleton. Membrane. Cytoplasmic in a soluble and membrane-associated form. - Information by UniProt
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Database links
- Entrez Gene: 3728 Human
- Omim: 173325 Human
- SwissProt: P14923 Human
- Unigene: 514174 Human
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Alternative names
- ARVD 12 antibody
- ARVD12 antibody
- Catenin (cadherin associated protein), gamma 80kDa antibody
see all
Images
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Western blot analysis of gamma-Catenin immunoprecipitated from lysates prepared from A431 cells treated with pervanadate. The immunoprecipitation was performed using mouse monoclonal anti-gamma-Catenin. The blots were probed with anti-phospho-gamma-Catenin (Tyr-550) antibody. The immunoprecipitated gamma-Catenin was untreated (lane 1) or treated with alkaline phosphatase (lane 2) then probed with the antibody. In addition, the anti-phospho-gamma-Catenin (Tyr-550) was used in the presence of phospho-gamma-Catenin (Tyr-550) peptide (lane 3) or phospho-gamma-Catenin (Tyr-644) peptide (lane 4).
Datasheets and documents
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Datasheet download
References (0)
ab29041 has not yet been referenced specifically in any publications.