Immunocytochemistry/ Immunofluorescence abreview for Anti-gamma H2A.X (phospho S139) antibody [9F3]

Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (spermatocytes)
Specification
spermatocytes
Fixative
Formaldehyde
Permeabilization
Yes - Triton X100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.15% · Temperature: 25°C

Other product details

Dilution
1/100
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS + 0.15% BSA+0.1% Tween 20

Secondary antibody

Name
Non-Abcam antibody was used: anti-mouse IgG
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Dilution
1/100

Additional data

PubMed ID
19403564: View

Mr. Pinton Alain

Verified customer

Submitted Aug 25 2009

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