Western blot abreview for Anti-gamma H2A.X (phospho S139) antibody [9F3]

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - nuclear (Human vascular smooth muscle cells treated with H2)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Human vascular smooth muscle cells treated with H2
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Concentration
1 µg/ml

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
Fluorescence
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 15 kDa

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Submitted Aug 16 2018

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