Immunocytochemistry/ Immunofluorescence abreview for Anti-gamma H2A.X (phospho S139) antibody [9F3]

Poor
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Hydractinia echinata Cell (head tissue fragment)
Permeabilization
Yes - 0.3% triton+1XPBS
Specification
head tissue fragment
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Fixative
Paraformaldehyde

Other product details

Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA+0.3%triton in 1X PBS
Dilution
1/500

Secondary antibody

Dilution
1/500

Additional data

Abcam response
Thank you for your valuable feedback. We have not previously tested this antibody in Hydractinia. We were unsure what the result might be, and we appreciate the time and effort that the reviewer has put in to testing this product in this species.

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Verified customer

Submitted Sep 04 2018

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