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Epigenetics and Nuclear Signaling Histones Variants
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RecombinantRabMAb

Recombinant Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)

  • Datasheet
  • SDS
Reviews (16) Submit a question References (125)

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Western blot - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)
  • Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)
  • Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)
  • Dot Blot - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)
  • Immunoprecipitation - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)
  • Western blot - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)
  • Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP854(2)Y] to gamma H2A.X (phospho S139)
  • Suitable for: IP, IHC-P, WB, ICC/IF, Dot blot
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free HRP

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Overview

  • Product name

    Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]
    See all gamma H2A.X primary antibodies
  • Description

    Rabbit monoclonal [EP854(2)Y] to gamma H2A.X (phospho S139)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WB, ICC/IF, Dot blotmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2 cell lysate treated with etoposide; Jurkat cell lysate. IHC-P: Human kidney transitional cell carcinoma, human brain, human, rat and mouse testis. ICC/IF: H2O2 treated HeLa cells. IP: HepG2 treated with etoposide and TSA whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP854(2)Y
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • Variants
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • DNA Damage Recognition
    • Epigenetics and Nuclear Signaling
    • Histones
    • H2A
    • Unmodified
    • Signal Transduction
    • Antibodies
    • gamma h2a.x

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab195188)
    • Alexa Fluor® 647 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab195189)
    • HRP Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab195190)
    • Alexa Fluor® 594 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab206898)
    • Alexa Fluor® 555 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab206900)
    • Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] - BSA and Azide free (ab215967)
  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Human gamma H2A.X (phospho S139) peptide (ab15645)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab81299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/40.
IHC-P (6)
Use at an assay dependent concentration.
WB (3)
1/5000 - 1/10000. Predicted molecular weight: 15 kDa.
ICC/IF (3)
1/250.
Dot blot
1/1000.
Notes
IP
1/40.
IHC-P
Use at an assay dependent concentration.
WB
1/5000 - 1/10000. Predicted molecular weight: 15 kDa.
ICC/IF
1/250.
Dot blot
1/1000.

Target

  • Function

    Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
  • Sequence similarities

    Belongs to the histone H2A family.
  • Developmental stage

    Synthesized in G1 as well as in S-phase.
  • Domain

    The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.
  • Post-translational
    modifications

    Phosphorylated on Ser-140 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Phosphorylation can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. Widespread phosphorylation may also serve to amplify the damage signal or aid repair of persistent lesions. Phosphorylation of Ser-140 (H2AX139ph) in response to ionizing radiation is mediated by both ATM and PRKDC while defects in DNA replication induce Ser-140 phosphorylation (H2AX139ph) subsequent to activation of ATR and PRKDC. Dephosphorylation of Ser-140 by PP2A is required for DNA DSB repair. In meiosis, Ser-140 phosphorylation (H2AX139ph) may occur at synaptonemal complexes during leptotene as an ATM-dependent response to the formation of programmed DSBs by SPO11. Ser-140 phosphorylation (H2AX139ph) may subsequently occurs at unsynapsed regions of both autosomes and the XY bivalent during zygotene, downstream of ATR and BRCA1 activation. Ser-140 phosphorylation (H2AX139ph) may also be required for transcriptional repression of unsynapsed chromatin and meiotic sex chromosome inactivation (MSCI), whereby the X and Y chromosomes condense in pachytene to form the heterochromatic XY-body. During immunoglobulin class switch recombination in lymphocytes, Ser-140 phosphorylation (H2AX139ph) may occur at sites of DNA-recombination subsequent to activation of the activation-induced cytidine deaminase AICDA. Phosphorylation at Tyr-143 (H2AXY142ph) by BAZ1B/WSTF determines the relative recruitment of either DNA repair or pro-apoptotic factors. Phosphorylation at Tyr-143 (H2AXY142ph) favors the recruitment of APBB1/FE65 and pro-apoptosis factors such as MAPK8/JNK1, triggering apoptosis. In contrast, dephosphorylation of Tyr-143 by EYA proteins (EYA1, EYA2, EYA3 or EYA4) favors the recruitment of MDC1-containing DNA repair complexes to the tail of phosphorylated Ser-140 (H2AX139ph).
    Monoubiquitination of Lys-120 (H2AXK119ub) by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
  • Cellular localization

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P16104 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3014 Human
    • Entrez Gene: 15270 Mouse
    • Entrez Gene: 500987 Rat
    • Omim: 601772 Human
    • SwissProt: P16104 Human
    • SwissProt: P27661 Mouse
    • Unigene: 477879 Human
    • Unigene: 245931 Mouse
    • Alternative names

      • H2A histone family member X antibody
      • H2A histone family member X antibody
      • H2A.FX antibody
      • H2A.X antibody
      • H2a/x antibody
      • H2AFX antibody
      • H2AX antibody
      • H2AX_HUMAN antibody
      • Histone H2A.X antibody
      see all

    Images

    • Western blot - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)
      Western blot - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)
      All lanes : Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299) at 1/100000 dilution

      Lane 1 : HepG2 cell lysate – treated with etoposide
      Lane 2 : HepG2 cell lysate – untreated

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 15 kDa



      Blocking buffer - 5% NFDM/TBST

      Diluting buffer - 1% BSA

    • Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)
      Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)Ge H et al., PLoS One, 7, e51821, 2012 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Immunofluorescence staining of A549 (Human lung carcinoma cell line) labeling gamma H2A.X (phospho S139) (green) with ab81299.

      Cells were fixed in 4% paraformaldehyde for 15 minutes and permeabilized in PBS-0.2% Triton for 10 minutes. After blocked for 1 hour, primary antibody was  diluted in blocking buffer (1/100) and incubated with fixed cells overnight at 4°C. Cells were washed and incubated with secondary antibodies (1/100) for 1 hour at room temperature. All slides were counterstained with 4′,6-diamidino-2-phenylindole (DAPI). Immunofluorescence was performed using confocal laser scanning microscopy (Lecia) or fluorescence microscopy (Olympus).

      Dexamethasone, DEX; Cisplatin, DDP.

    • Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)
      Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)

      Immunocytochemistry/Immunofluorescence analysis of HeLa cells (untreated and treated with H2O2) labelling Histone H2A.X (phospho S139) with ab81299 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab7291, a mouse anti-tubulin (1/1000) using ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) as the secondary antibody. Nuclei counterstained with DAPI (blue). 

      Control 1: primary antibody (1/250) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)Image courtesy of Carl Hobbs (Kings College London, United Kingdom)

      IHC-P image of γH2A.X staining on Rat testis sections using unpurified ab81299 (1:5000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab81299 was diluted 1:5000 in TBS buffer (containing BSA and Azide) and sections were then incubated with unpurified ab81299 for 2 hours at 21°C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG (1:250).

      See Abreview

    • Dot Blot - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)
      Dot Blot - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)

      Dot blot analysis of Histone H2A.X single phospho peptide pS139 (lane 1) and Histone H2A.X non-phospho peptide (lane 2) with ab81299 at 1/1000. Blocking and diluting buffer was 5% NFDM/TBST. The secondary antibody used was ab97051 Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) at 1/100,000.

    • Immunoprecipitation - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)
      Immunoprecipitation - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)

      ab81299 at 1/40 immunoprecipitating Histone H2A.X (phospho S139) in HepG2 (human hepatocellular carcinoma epithelial) whole cell lysate observed at 15 KDa (lanes 1 and 2).

      Lane 1 (input): HepG2 treated with etoposide and TSA whole cell lysate 10μg
      Lane 2 (+): ab81299 + HepG2 treated with etoposide and TSA whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab81299 in HepG2 treated with etoposide and TSA

      For western blotting, ab81299 (Purified) at 1/200 dilution and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)
      Western blot - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)
      All lanes : Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299) at 1/500000 dilution (unpurified)

      Lane 1 : Jurkat cell lysate - untreated
      Lane 2 : Jurkat cell lysate - treated with etoposide

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP Labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 15 kDa
      Observed band size: 15 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)Image courtesy of Carl Hobbs (Kings College London, United Kingdom)

      IHC-P image of γH2A.X staining on Mouse testis sections using unpurified ab81299 (1:5000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab81299 was diluted 1:5000 in TBS buffer (containing BSA and Azide) and sections were then incubated with unpurified ab81299 for 2 hours at 21°C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG (1:250).

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)

      Immunohistochemical staining of paraffin embedded human brain with purified ab81299 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)

      Immunohistochemical staining of paraffin embedded human brain with unpurified ab81299 at a working dilution of 1 in 50. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)

      Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human kidney transitional cell carcinoma using unpurified ab81299 at a dilution of 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)This image is courtesy of an Abreview submitted by Carl Hobbs.

      ab81299 staining H2A.X in Human Testis tissue sections by Immunohistochemistry (Formalin/PFA fixed paraffin embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in Citric acid. Samples were incubated with primary antibody (1/50 in TBS) for 2 hours at 21°C. A biotin conjugated Anti-Rabbit IgG (goat polyclonal) was used as the secondary antibody at a 1/250 dilution.

      See Abreview

    • Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)
      Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab81299)

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (125)

    Publishing research using ab81299? Please let us know so that we can cite the reference in this datasheet.

    ab81299 has been referenced in 125 publications.

    • Sakamoto Y  et al. Mitotic cells can repair DNA double-strand breaks via a homology-directed pathway. J Radiat Res 62:25-33 (2021). PubMed: 33009557
    • Guo Q  et al. STING promotes senescence, apoptosis, and extracellular matrix degradation in osteoarthritis via the NF-?B signaling pathway. Cell Death Dis 12:13 (2021). PubMed: 33414452
    • Elbakry A  et al. ATRX and RECQ5 define distinct homologous recombination subpathways. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33431668
    • Shaalan AK  et al. Inhibition of Aurora Kinase B activity disrupts development and differentiation of salivary glands. Cell Death Discov 7:16 (2021). PubMed: 33462217
    • Cai T  et al. Notoginsenoside R1 induces DNA damage via PHF6 protein to inhibit cervical carcinoma cell proliferation. Mol Med Rep 23:N/A (2021). PubMed: 33537816
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 16 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Testes)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution (S1699)
    Permeabilization
    Yes - Wash buffer, blocking solution and secondary diluent was 1X TBS with 0.1% Tween-20
    Specification
    Testes
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    10% neutral buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 22 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H2A.X (phospho S139) antibody [EP854(2)Y] - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Na-citrate pH6
    Sample
    Mouse Tissue sections (Liver, P5)
    Specification
    Liver, P5
    Permeabilization
    Yes - Triton 0,05%
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 07 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H2A.X (phospho S139) [EP854(2)Y] antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Sample
    Human Cell (HEK293)
    Specification
    HEK293
    Permeabilization
    Yes - triton
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 24 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H2A.X (phospho S139) antibody [EP854(2)Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Testis)
    Specification
    Testis
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Read More

    MR. Carl Hobbs

    Verified customer

    Submitted May 15 2013

    Immunohistochemistry (Frozen sections) abreview for Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Rat Tissue sections (retina))
    Permeabilization
    Yes - 0.3% Triton X100
    Specification
    Rat Tissue sections (retina)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 11 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Dog Cell (canine mammary gland tumor)
    Permeabilization
    Yes - 0.1 % Triton X-100
    Specification
    canine mammary gland tumor
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    MISS. HuiSu Kim

    Verified customer

    Submitted Oct 05 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (heart)
    Permeabilization
    Yes - Triton-X100 0.1%
    Specification
    heart
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 14 2021

    Immunohistochemistry (Frozen sections) abreview for Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (retina)
    Permeabilization
    Yes - 0.3% Triton X100
    Specification
    retina
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    4% PFA
    Read More

    Ms. jung eun woo

    Verified customer

    Submitted Aug 31 2018

    ChIP abreview for Anti-Histone H2A.X (phospho S139) antibody [EP854(2)Y] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - nuclear (human u2os cells)
    Negative control
    sample without dna damage
    Specification
    human u2os cells
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 15 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 08 2016

    Western blot abreview for Anti-Histone H2A.X (phospho S139) antibody [EP854(2)Y] - ChIP Grade

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Recombinant protein (C-terminus of H2A.X with full range of phosphoryla)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (Dot blot)
    Loading amount
    0.384 µg
    Specification
    C-terminus of H2A.X with full range of phosphoryla
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Peter Ellis

    Verified customer

    Submitted Jun 02 2016

    1-10 of 16 Abreviews or Q&A

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