Overview

  • Product name

    Anti-gamma Sarcoglycan antibody [EPR17862-40]
    See all gamma Sarcoglycan primary antibodies
  • Description

    Rabbit monoclonal [EPR17862-40] to gamma Sarcoglycan
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human gamma Sarcoglycan aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: Q13326

  • Positive control

    • WB: Human skeletal muscle, MCF-7, Human fetal brain, Human fetal heart, Human fetal spleen or Human fetal kidney whole cell lysate. IHC-P: Human skeletal muscle.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17862-40
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab203112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/50.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).

Target

  • Function

    Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
  • Tissue specificity

    Expressed in skeletal and heart muscle.
  • Involvement in disease

    Defects in SGCG are the cause of limb-girdle muscular dystrophy type 2C (LGMD2C) [MIM:253700]. LGMD2C is characterized by progressive muscle wasting from early childhood.
  • Sequence similarities

    Belongs to the sarcoglycan beta/delta/gamma/zeta family.
  • Cellular localization

    Cell membrane > sarcolemma. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • 35 kDa dystrophin associated glycoprotein antibody
    • 35 kDa dystrophin-associated glycoprotein antibody
    • 35DAG antibody
    • 35kD dystrophin associated glycoprotein antibody
    • 35kDa dystrophin-associated glycoprotein antibody
    • A4 antibody
    • DAGA4 antibody
    • DMDA antibody
    • DMDA1 antibody
    • Gamma SG antibody
    • Gamma-sarcoglycan antibody
    • Gamma-SG antibody
    • LGMD2C antibody
    • MAM antibody
    • MGC130048 antibody
    • Sarcoglycan gamma antibody
    • SCARMD2 antibody
    • SCG3 antibody
    • SGCG antibody
    • SGCG_HUMAN antibody
    • TYPE antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling gamma Sarcoglycan with ab203112 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. Cell membrane and weakly cytoplasmic staining on Human skeletal muscle tissue is observed. Counter stained with Hematoxylin.

     

    Negative control: Used PBS instead of primary antibody, secondary antibody is ab97051 Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112) at 1/10000 dilution + Human skeletal muscle lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 32 kDa
    Observed band size: 32 kDa


    Exposure time: 15 seconds


    Blocking/diluting buffer 5% NFDM/TBST

     

    This protein is expressed in skeletal and heart muscle.

  • Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112) at 1/2000 dilution + MCF-7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µl

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 32 kDa
    Observed band size: 32 kDa


    Exposure time: 3 minutes


    Blcking/Dilution buffer 5% NFDM/TBST

     

    This protein is expressed in skeletal and heart muscle.

  • All lanes : Anti-gamma Sarcoglycan antibody [EPR17862-40] (ab203112) at 1/2000 dilution

    Lane 1 : Human fetal brain whole cell lysate
    Lane 2 : Human fetal heart whole cell lysate
    Lane 3 : Human fetal kidney whole cell lysate
    Lane 4 : Human fetal spleen whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 32 kDa
    Observed band size: 32 kDa



    Blocking/DIlution buffer 5% NFDM/TBST.

    ab181602 Anti-GAPDH antibody [EPR16891] used as a control.

    This protein is expressed in skeletal and heart muscle.

  • gamma Sarcoglycan was immunoprecipitated from 1mg of Human fetal kidney whole cell lysate with ab203112 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab203112 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Human fetal kidney whole cell lysate10 µg (Input). Lane 2: ab203112 IP in Human fetal kidney whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203112 in Human fetal kidney whole cell lysate.
    Exposure time: 3 seconds

    Blocking/Dilution buffer 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling gamma Sarcoglycan with ab203112 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. Counter stained with Hematoxylin. No staining on Human colon tissue is observed.

     

    Negative control: Used PBS instead of primary antibody, secondary antibody is ab97051 Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab203112 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Muscle)
Permeabilization
No
Specification
Muscle
Blocking step
(agent) for 30 minute(s) · Concentration: 0.5% · Temperature: 24°C
Fixative
Acetone

Dr. Amir Dori

Verified customer

Submitted Mar 14 2017

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