Recombinant
RabMAb

Recombinant Anti-gamma Sarcoglycan antibody [EPR17862-40] - BSA and Azide free (ab240365)

Overview

  • Product name

    Anti-gamma Sarcoglycan antibody [EPR17862-40] - BSA and Azide free
    See all gamma Sarcoglycan primary antibodies
  • Description

    Rabbit monoclonal [EPR17862-40] to gamma Sarcoglycan - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human gamma Sarcoglycan aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: Q13326

  • General notes

    Ab240365 is the carrier-free version of ab203112. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240365 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
  • Tissue specificity

    Expressed in skeletal and heart muscle.
  • Involvement in disease

    Defects in SGCG are the cause of limb-girdle muscular dystrophy type 2C (LGMD2C) [MIM:253700]. LGMD2C is characterized by progressive muscle wasting from early childhood.
  • Sequence similarities

    Belongs to the sarcoglycan beta/delta/gamma/zeta family.
  • Cellular localization

    Cell membrane > sarcolemma. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • 35 kDa dystrophin associated glycoprotein antibody
    • 35 kDa dystrophin-associated glycoprotein antibody
    • 35DAG antibody
    • 35kD dystrophin associated glycoprotein antibody
    • 35kDa dystrophin-associated glycoprotein antibody
    • A4 antibody
    • DAGA4 antibody
    • DMDA antibody
    • DMDA1 antibody
    • Gamma SG antibody
    • Gamma-sarcoglycan antibody
    • Gamma-SG antibody
    • LGMD2C antibody
    • MAM antibody
    • MGC130048 antibody
    • Sarcoglycan gamma antibody
    • SCARMD2 antibody
    • SCG3 antibody
    • SGCG antibody
    • SGCG_HUMAN antibody
    • TYPE antibody
    see all

Images

  • gamma Sarcoglycan was immunoprecipitated from 1mg of Human fetal kidney whole cell lysate with ab203112 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab203112 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Human fetal kidney whole cell lysate10 µg (Input). Lane 2: ab203112 IP in Human fetal kidney whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203112 in Human fetal kidney whole cell lysate.
    Exposure time: 3 seconds

    Blocking/Dilution buffer 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203112).

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling gamma Sarcoglycan with ab203112 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. Counter stained with Hematoxylin. No staining on Human colon tissue is observed.

     

    Negative control: Used PBS instead of primary antibody, secondary antibody is ab97051 Goat Anti-Rabbit IgG H&L (HRP).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203112).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling gamma Sarcoglycan with ab203112 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. Cell membrane and weakly cytoplasmic staining on Human skeletal muscle tissue is observed. Counter stained with Hematoxylin.

     

    Negative control: Used PBS instead of primary antibody, secondary antibody is ab97051 Goat Anti-Rabbit IgG H&L (HRP).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203112).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab240365 has not yet been referenced specifically in any publications.

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