Overview

  • Product name
    Anti-gamma Synuclein antibody [1H10D2]
    See all gamma Synuclein primary antibodies
  • Description
    Mouse monoclonal [1H10D2] to gamma Synuclein
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Ni-NTA purified truncated recombinant human SNCG (a.a. 2-127) expressed in E. Coli strain BL21 (DE3)

  • Positive control
    • Human ovarian carcinoma tissue, human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab47966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/500 - 1/2000.
ELISA 1/10000.
Flow Cyt Use 2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway.
  • Tissue specificity
    Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung.
  • Sequence similarities
    Belongs to the synuclein family.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases.
  • Cellular localization
    Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle.
  • Information by UniProt
  • Database links
  • Alternative names
    • BCSG1 antibody
    • Breast cancer specific gene 1 protein antibody
    • Breast cancer specific protein 1 antibody
    • Breast cancer-specific gene 1 protein antibody
    • Gamma synuclein antibody
    • Gamma-synuclein antibody
    • Persyn antibody
    • PRSN antibody
    • SNCG antibody
    • SR antibody
    • Synoretin antibody
    • Synuclein gamma antibody
    • synuclein, gamma (breast cancer-specific protein 1) antibody
    • SYUG_HUMAN antibody
    see all

Images

  • Anti-gamma Synuclein antibody [1H10D2] (ab47966) at 1/500 dilution + Truncated gamma Synuclein recombinant protein

    Predicted band size: 13 kDa
    Observed band size: 13 kDa

  • This image shows cytoplasmic staining of Human ovarian carcinoma tissue treated with ab47966 at 1/500 dilution.
  • This image shows cytoplasmic and nuclear staining of Human breast carcinoma tissue treated with ab47966 at 1/500 dilution.
  • Overlay histogram showing SH-SY5Y cells stained with ab47966 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab47966, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References

This product has been referenced in:
  • Majbour NK  et al. Oligomeric and phosphorylated alpha-synuclein as potential CSF biomarkers for Parkinson's disease. Mol Neurodegener 11:7 (2016). Read more (PubMed: 26782965) »
  • Boyer F & Dreyer JL The role of gamma-synuclein in cocaine-induced behaviour in rats. Eur J Neurosci 27:2938-51 (2008). WB, IHC-Fr ; Rat . Read more (PubMed: 18588534) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxxx. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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Answer

Thank you for contacting Abcam. At Abcam, providing quality service is just as important as providing quality products. Our Abpromise® ensures that you can trust our products to perform as stated on the datasheets, or we will offer a replacement, credit, or refund. For our records, could you please send me the protocol that you have used with ab47966. Including cell preparation, blocking solutions, times and temperature as well as antibody incubation times and any controls that you may have used. If possible, would you be able find the lot number of the previous vial or an order number from that purchase as well? The Abcam Abpromise® guarantee: • 100% Scientific and Customer Support for any product you buy from Abcam or one of our authorized distributors. • We guarantee our products work in the tested species and applications stated on the datasheet. • We will replace or refund products not performing as stated on the datasheet if reported within 6 months of purchase. • We investigate any quality concerns raised by customers to ensure our catalog contains products that perform to the highest standards. Please note these conditions to our Abpromise®: • Protocol information must be provided in order for the claim to be validated. • Antibodies tested in recombinant samples only are not guaranteed for use on endogenous samples. • “Predicted to react” information on the datasheets is provided for reference only and these species are not guaranteed. • Fast Track antibodies are covered for ELISA against the immunizing peptide only. I look forward to hearing back form you and working through this issue with you. I hope this information is helpful, but please do not hesitate to contact us with further questions.

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