- Datasheet
- Specific References (9)
- Protocols
Overview
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Product nameAnti-gamma Synuclein antibody
See all gamma Synuclein primary antibodies -
DescriptionRabbit polyclonal to gamma Synuclein
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Host speciesRabbit
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SpecificityThe immunizing peptide was chosen to avoid cross-reactivity of the antiserum to other synucleins. Immunohistochemical and western blot analysis of human brain indicates a high level of specificity for this antiserum.
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Tested applicationsSuitable for: ICC/IF, IP, IHC-P, IHC-Fr, WBmore details
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Species reactivityReacts with: Rat, Human
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Immunogen
Synthetic peptide
EKEEVAEEAQSGGD
corresponds to amino acids 114-127 of the human gamma synuclein protein. The conjugate was cross-linked by maleimidocaproyl-N-hydroxy succinimide (MSC) and coupled to diphtheria toxoid.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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PurityWhole antiserum
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab6169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | ||
| IP | ||
| IHC-P | ||
| IHC-Fr | ||
| WB |
Application notes
ICC/IF: 1/100 - 1/150.
IHC-P: 1/100.
IHC-Fr: Use at an assay dependent dilution.
WB: 1/500 - 1/1000.
1 site ELISA: Not tested.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100.
IHC-Fr: Use at an assay dependent dilution.
WB: 1/500 - 1/1000.
1 site ELISA: Not tested.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionPlays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway.
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Tissue specificityHighly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung.
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Sequence similaritiesBelongs to the synuclein family.
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Post-translational
modificationsPhosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases. -
Cellular localizationCytoplasm > perinuclear region. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle.
- Information by UniProt
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Database links
- Entrez Gene: 6623 Human
- Entrez Gene: 64347 Rat
- Omim: 602998 Human
- SwissProt: O76070 Human
- SwissProt: Q63544 Rat
- Unigene: 349470 Human
- Unigene: 2883 Rat
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Alternative names
- BCSG1 antibody
- Breast cancer specific gene 1 protein antibody
- Breast cancer specific protein 1 antibody
see all
Images
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Western blot - Anti-gamma Synuclein antibody (ab6169)ab6169 was tested on recombinant protein only, 100ng recombinant protein each lane. 1= alpha-synuclein, 2 = beta-synuclein, 3 = gamma-synuclein. The primary was 1/2000, secondary was alkaline phosphatase conjugated anti-rabbit from Sigma. We did not try it on crude samples such as brain or other tissue homogenates.
ab6169 was tested on recombinant protein only, 100ng recombinant protein each lane. 1= alpha-synuclein, 2 = beta-synuclein, 3 = gamma-synuclein. The primary was 1/2000, secondary was alkaline phosphatase conjugated anti-rabbit from Sigma. We did not try it on crude samples such as brain or other tissue homogenates.
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Immunocytochemistry/ Immunofluorescence - Anti-gamma Synuclein antibody (ab6169)ICC/IF image of ab6169 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6169, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Synuclein antibody (ab6169)IHC image of ab6169 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6169, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
References
This product has been referenced in:
- He X et al. Long non-coding RNA AK058003, as a precursor of miR-15a, interacts with HuR to inhibit the expression of ?-synuclein in hepatocellular carcinoma cells. Oncotarget 8:9451-9465 (2017). WB, ICC/IF, Other ; Human . Read more (PubMed: 28035067) »
- Newman AJ et al. A New Method for Quantitative Immunoblotting of Endogenous a-Synuclein. PLoS One 8:e81314 (2013). WB ; Human . Read more (PubMed: 24278419) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"