Product namegamma Synuclein/SNCG overexpression 293T lysate (whole cell)
ab94101 is a 293T cell transfected lysate in which Human gamma Synuclein/SNCG has been transiently over-expressed using a pCMV-gamma Synuclein/SNCG plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituent: 100% 1x Sample Buffer
Concentration information loading...
BackgroundFunction: Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway. Tissue specificity: Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung. Similarity: Belongs to the synuclein family. PTM: Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases.
Our Abpromise guarantee covers the use of ab94101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94101 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-gamma Synuclein/SNCG antibody (ab89462) at 1/500 dilution
Lane 1 :
gamma Synuclein/SNCG overexpression 293T lysate (whole cell) (ab94101)
Lane 2 : 293T Non Transfected Lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94101 has not yet been referenced specifically in any publications.