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    gamma-tubulin-antibody-4d11-c-terminal-ab173831.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microtubules Tubulin
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Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)

  • Datasheet
  • SDS
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Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)
  • Western blot - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)

Key features and details

  • Mouse monoclonal [4D11] to gamma Tubulin - C-terminal
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Human, African green monkey
  • Isotype: IgG2b

You may also be interested in

Protein
Product image
Recombinant Human gamma Tubulin protein (denatured) (ab115710)
Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-gamma Tubulin antibody [4D11] - C-terminal
    See all gamma Tubulin primary antibodies
  • Description

    Mouse monoclonal [4D11] to gamma Tubulin - C-terminal
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human, African green monkey
  • Immunogen

    Recombinant fragment within Human gamma Tubulin aa 437-451 (C terminal). The exact sequence is proprietary.
    Sequence:

    AATRPDYISWGTQEQ


    Database link: P23258
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human breast carcinoma and colon tissues; HeLa and U2OS cells; HeLa, HepG2, U2OS and K562 cell lysates; mouse 3T3L1 and spleen lysates; monkey COS7 cell lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    4D11
  • Isotype

    IgG2b
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Centrosome

Associated products

  • Alternative Versions

    • Anti-ATP5D antibody [4D11] (ab174438)
    • HRP Anti-6X His tag® antibody [4D11] (ab202004)
    • Anti-6X His tag® antibody [4D11] (ab5000)
    • Anti-hnRNP L antibody [4D11] (ab6106)
    • FITC Anti-LLGL1 antibody [4D11] (ab95802)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Hep G2 whole cell lysate (ab166833)
    • Mouse spleen tissue lysate - total protein (ab29293)
    • HeLa whole cell lysate (ab29545)
    • K-562 whole cell lysate (ab7911)
    • Mouse spleen tissue lysate - total protein (ab7937)
  • Recombinant Protein

    • Recombinant Human gamma Tubulin protein (denatured) (ab115710)
  • Related Products

    • Recombinant Human gamma Tubulin protein (denatured) (ab115710)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab173831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 51 kDa.
ICC/IF
1/50.
IHC-P
1/20 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000. Predicted molecular weight: 51 kDa.
ICC/IF
1/50.
IHC-P
1/20 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.
  • Sequence similarities

    Belongs to the tubulin family.
  • Post-translational
    modifications

    Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome.
  • Target information above from: UniProt accession Q9NRH3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 27175 Human
    • Entrez Gene: 7283 Human
    • Entrez Gene: 103733 Mouse
    • Entrez Gene: 103768 Mouse
    • Omim: 191135 Human
    • Omim: 605785 Human
    • SwissProt: P23258 Human
    • SwissProt: Q9NRH3 Human
    • SwissProt: P83887 Mouse
    • SwissProt: Q8VCK3 Mouse
    • Unigene: 279669 Human
    • Unigene: 708059 Human
    • Unigene: 142348 Mouse
    • Unigene: 479145 Mouse
    see all
  • Alternative names

    • Gamma 1 tubulin antibody
    • Gamma 2 tubulin antibody
    • Gamma Tubulin 1 antibody
    • Gamma Tubulin 2 antibody
    • Gamma tubulin complex component 1 antibody
    • Gamma-2-tubulin antibody
    • GCP 1 antibody
    • GCP-1 antibody
    • GCP1 antibody
    • MGC131994 antibody
    • TBG2_HUMAN antibody
    • TUBG antibody
    • TUBG1 antibody
    • TUBG2 antibody
    • TUBGCP1 antibody
    • Tubulin gamma 1 chain antibody
    • Tubulin gamma 2 chain antibody
    • Tubulin gamma complex-associated protein 1 antibody
    • Tubulin gamma-2 chain antibody
    • tubulin, gamma 1 antibody
    • tubulin, gamma 2 antibody
    • tubulin, gamma polypeptide antibody
    • Xgam antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)
    Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)

    Immunofluorescence analysis of formalin-fixed, permeabilized A) HeLa cells and B) U2OS, labeling gamma Tubulin (green) using ab173831 at a 1/50 dilution. Cells were washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. F-Actin (red) was stained with DyLight 547 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.

  • Western blot - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)
    Western blot - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)
    All lanes : Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : U2OS cell lysate
    Lane 4 : K562 cell lysate
    Lane 5 : mouse 3T3L1 cell lysate
    Lane 6 : mouse spleen lysate
    Lane 7 : monkey COS7 cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : goat anti-mouse-HRP at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 51 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)

    Immunohistochemical analysis of Human breast carcinoma tissue, labeling gamma Tubulin with ab173831 at a 1/20 dilution (left panel) or without primary antibody (right panel). To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [4D11] - C-terminal (ab173831)

    Immunohistochemical analysis of Human colon tissue, labeling gamma Tubulin with ab173831 at a 1/200 dilution (left panel) or without primary antibody (right panel). To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab173831? Please let us know so that we can cite the reference in this datasheet.

ab173831 has been referenced in 1 publication.

  • Cole BK  et al. An In Vitro Cynomolgus Vascular Surrogate System for Preclinical Drug Assessment and Human Translation. Arterioscler Thromb Vasc Biol 35:2185-95 (2015). PubMed: 26293464

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