Key features and details
- Rabbit polyclonal to gamma Tubulin
- Suitable for: ICC/IF, WB
- Reacts with: Chicken, Human
- Isotype: IgG
Product nameAnti-gamma Tubulin antibody
See all gamma Tubulin primary antibodies
DescriptionRabbit polyclonal to gamma Tubulin
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Chicken, Human
Predicted to work with: Drosophila melanogaster, Schizosaccharomyces pombe, Sea urchin
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
Our Abpromise guarantee covers the use of ab11317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
It is recommended to fix cells with methanol/acetone.
|WB||1/1000. Detects a band of approximately 48 kDa. This concentration was determined for A431 cultured cells extract. Block with 5% BSA in TBST (not milk). Detects a band of approximately 48 kDa. Additional bands can occasionally be seen.|
FunctionTubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.
Sequence similaritiesBelongs to the tubulin family.
modificationsPhosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
Cellular localizationCytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- Gamma 1 tubulin antibody
- Gamma 2 tubulin antibody
- Gamma Tubulin 1 antibody
Immunocytochemistry/Immunofluorescence analysis of NIH3T3 cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).
All lanes : Anti-gamma Tubulin antibody (ab11317) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : C2C12 cell lysate
Lane 5 : Rat2 cell lysate
Lane 6 : U87 cell lysate
Lane 7 : NIH3T3 cell lysate
Lane 8 : CHO cell lysate
All lanes : Peroxidase-conjugated goat anti-rabbit IgG
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).
Immunocytochemistry/Immunofluorescence analysis of Rat2 cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).
ab11317 has been referenced in 67 publications.
- Burns TA et al. A Novel Mouse Model for Cilia-Associated Cardiovascular Anomalies with a High Penetrance of Total Anomalous Pulmonary Venous Return. Anat Rec (Hoboken) 302:136-145 (2019). PubMed: 30289203
- Lafarga V et al. TIAR marks nuclear G2/M transition granules and restricts CDK1 activity under replication stress. EMBO Rep 20:N/A (2019). PubMed: 30538118
- Joseph DB et al. Epithelial DNA methyltransferase-1 regulates cell survival, growth and maturation in developing prostatic buds. Dev Biol 447:157-169 (2019). PubMed: 30659795
- Patnaik SR et al. Bardet-Biedl Syndrome proteins regulate cilia disassembly during tissue maturation. Cell Mol Life Sci 76:757-775 (2019). PubMed: 30446775
- Jiao AL et al. Human nuclear RNAi-defective 2 (NRDE2) is an essential RNA splicing factor. RNA 25:352-363 (2019). PubMed: 30538148