Product nameAnti-gamma Tubulin antibody
See all gamma Tubulin primary antibodies
DescriptionRabbit polyclonal to gamma Tubulin
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Chicken, Hamster, Human, African green monkey, Tribolium castaneum
Predicted to work with: Drosophila melanogaster, Schizosaccharomyces pombe, Sea urchin
- WB: Recombinant Human gamma Tubulin protein (denatured) (ab115710), A431, HeLa, Jurkat, C2C12, Rat2, U87, NIH3T3 and CHO cell lysates. ICC/IF: NIH3T3, HeLa and Rat2 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
Our Abpromise guarantee covers the use of ab11317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
It is recommended to fix cells with methanol/acetone.
|WB||1/1000. Detects a band of approximately 48 kDa. This concentration was determined for A431 cultured cells extract. Block with 5% BSA in TBST (not milk). Detects a band of approximately 48 kDa. Additional bands can occasionally be seen.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionTubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.
Sequence similaritiesBelongs to the tubulin family.
modificationsPhosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
Cellular localizationCytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- Gamma 1 tubulin antibody
- Gamma 2 tubulin antibody
- Gamma Tubulin 1 antibody
Immunocytochemistry/Immunofluorescence analysis of NIH3T3 cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).
All lanes : Anti-gamma Tubulin antibody (ab11317) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : C2C12 cell lysate
Lane 5 : Rat2 cell lysate
Lane 6 : U87 cell lysate
Lane 7 : NIH3T3 cell lysate
Lane 8 : CHO cell lysate
All lanes : Peroxidase-conjugated goat anti-rabbit IgG
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).
ab11317 staining gamma Tubulin in mouse embryonic brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in 1mM EDTA. Samples were incubated with primary antibody (1/500 in blocking buffer) for 1 hour at 20°C. An Alexa Fluor® 568-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
Immunocytochemistry/Immunofluorescence analysis of Rat2 cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).
This product has been referenced in:
- Burns TA et al. A Novel Mouse Model for Cilia-Associated Cardiovascular Anomalies with a High Penetrance of Total Anomalous Pulmonary Venous Return. Anat Rec (Hoboken) 302:136-145 (2019). Read more (PubMed: 30289203) »
- Lafarga V et al. TIAR marks nuclear G2/M transition granules and restricts CDK1 activity under replication stress. EMBO Rep 20:N/A (2019). Read more (PubMed: 30538118) »