Anti-gamma Tubulin antibody (ab11317)
Key features and details
- Rabbit polyclonal to gamma Tubulin
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human, Chinese hamster
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-gamma Tubulin antibody
See all gamma Tubulin primary antibodies -
Description
Rabbit polyclonal to gamma Tubulin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Chinese hamster
Predicted to work with: Drosophila melanogaster, Schizosaccharomyces pombe, Sea urchin
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Immunogen
Synthetic peptide:
EEFATEGTDRKDVFFY-C
conjugated to KLH, corresponding to N terminal amino acids 38-53 of Human gamma Tubulin -
Positive control
- ICC/IF: Rat2 cells; NIH3T3 cells; HeLa cells. WB: Rat2 cell lysate; A431 cell lysate; HeLa cell lysate; Jurkat cell lysate; U87 cell lysate; C2C12 cell lysate; CHO cell lysate; NIH3T3 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (5) |
1/5000.
It is recommended to fix cells with methanol/acetone. |
| WB | (7) |
1/1000. Detects a band of approximately 48 kDa. This concentration was determined for A431 cultured cells extract. Block with 5% BSA in TBST (not milk). Detects a band of approximately 48 kDa. Additional bands can occasionally be seen.
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| Notes |
|---|
|
ICC/IF
1/5000. It is recommended to fix cells with methanol/acetone. |
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WB
1/1000. Detects a band of approximately 48 kDa. This concentration was determined for A431 cultured cells extract. Block with 5% BSA in TBST (not milk). Detects a band of approximately 48 kDa. Additional bands can occasionally be seen. |
Target
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Function
Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. -
Sequence similarities
Belongs to the tubulin family. -
Post-translational
modificationsPhosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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Database links
- Entrez Gene: 35199 Drosophila melanogaster
- Entrez Gene: 27175 Human
- Entrez Gene: 7283 Human
- Entrez Gene: 103733 Mouse
- Entrez Gene: 103768 Mouse
- Entrez Gene: 252921 Rat
- Entrez Gene: 680991 Rat
- Omim: 191135 Human
see all -
Alternative names
- Gamma 1 tubulin antibody
- Gamma 2 tubulin antibody
- Gamma Tubulin 1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody (ab11317)
Immunocytochemistry/Immunofluorescence analysis of NIH3T3 cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).
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Western blot - Anti-gamma Tubulin antibody (ab11317)All lanes : Anti-gamma Tubulin antibody (ab11317) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : C2C12 cell lysate
Lane 5 : Rat2 cell lysate
Lane 6 : U87 cell lysate
Lane 7 : NIH3T3 cell lysate
Lane 8 : CHO cell lysate
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG -
Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody (ab11317)Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).
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Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody (ab11317)Immunocytochemistry/Immunofluorescence analysis of Rat2 cells labelling gamma Tubulin (green) with ab11317 at a dilution of 1/1500. A Atto448 dye conjugated goat anti-rabbit IgG was used as the seondary antibody. Actin was stained with phalloidin rhodamine conjugate (red). Nuclear staining with DAPI (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (88)
ab11317 has been referenced in 88 publications.
- Lau EO et al. DIAPH3 deficiency links microtubules to mitotic errors, defective neurogenesis, and brain dysfunction. Elife 10:N/A (2021). PubMed: 33899739
- Gestuveo RJ et al. Analysis of Zika virus capsid-Aedes aegypti mosquito interactome reveals pro-viral host factors critical for establishing infection. Nat Commun 12:2766 (2021). PubMed: 33986255
- Marquis C et al. Chromosomally unstable tumor cells specifically require KIF18A for proliferation. Nat Commun 12:1213 (2021). PubMed: 33619254
- Turn RE et al. Roles for ELMOD2 and Rootletin in ciliogenesis. Mol Biol Cell 32:800-822 (2021). PubMed: 33596093
- Ibañez-Vega J et al. Ecm29-Dependent Proteasome Localization Regulates Cytoskeleton Remodeling at the Immune Synapse. Front Cell Dev Biol 9:650817 (2021). PubMed: 34055780