Anti-gamma Tubulin antibody (ab11317)

I am very sorry to hear that you are having problems with this product (ab11317). It does appear you may be getting non specific binding with this primary antibody. I would like to suggest the following modifications to your protocol. You can decrease the amount of non specific binding by using a higher dilution of antibody. The dilutions stated on our datasheets are only a guideline. You could also try incubating for an hour at 4oC. Antibody binding is usually more efficient and specific at this lower temperature. Increasing the number of washes, and preparing the antibody buffer and washing buffers with a detergent can also help to wash out any unbound antibody. You could use Tween at 2% if you are not already doing so. Also, most of the reviews we have on this antibody have blocked using 4 or 5% BSA for 1 hour. If these suggestions do not improve your results, it could be that the secondary antibody you are using requires optimising for this particular primary antibody. I would suggest increasing the dilution of your GAR alexafluor 594. Please let me know if this helps and do not hesitate to contact us for further advice. With thanks

Thank you for your enquiry. The rabbit used is New Zealand White. This is a commonly used type for all antisera manufacturers. I hope this information helps. Please do not hesitate to contact us if you need anything further.

Thank you for your enquiry. None of these products have been specifically tested in the application IHC-P. Having spoken with the originator of Ab11316 it would appear that this product has only been tested on methanol fixed cells and not paraffin fixed sections (the data sheet has now been updated to reflect this additional information).Should you decide to go ahead and test this antibody please let us know how you get on and in return we will award you 50 points with the Abcam Loyalty Scheme, which can be redeemed on a number of rewards. You may wish to search for an Ab that has specifically been tested for use in IHC-P by using a search engine such as "Biocompare.com". I would like to thank you for bringing this matter to my attention. If you have any further questions then please do not hesitate to get back in touch with us.

According to my colleagues no other bands were recognised, using A431 cultured cells whole extract (the 1:1000 recommended dilution is for those particular cells, it must be adapted to other tissues and cell types). This suggest your protocol could be modified as I have previously suggested, or that in your cells the antibody detect other forms of the protein (for example small bands can be degraded gamma tubulin or not-posttranslationally modified protein, larger bands could be the preprotein)or a protein similar to your gamma tubulin. Please let me know if you need further advice,

I'm sorry to hear you are having problems with ab11317. Multiple bands can be caused by a number of factors in your protocol. Most importantly, do you get the 48KDa band you expect? Multiple bands can be caused by: -too much primary antibody -too much secondary antibody -wrong/too much blocking (alternate BSA and milk and see which one gives you best results, a 5%w/v concentration should be plenty) -degradation of the samples if they are not in an appropriate lysis buffer and kept on ice at all times -degradation of the antibody due to repeated freeze-thaw. Without a complete description of your protocol it is difficult to know which step might need altering. I hope these suggestions help,please do not hesitate to contact us if you need further advice,

Based on the assay information from the last 5 batches produced, this product has a concentration of 10-14mg/ml. Please do not hesitate to contact us if you need further information,