Key features and details
- Rabbit polyclonal to gamma Tubulin
- Suitable for: IHC-P, IHC-Fr, ICC/IF, IHC (Methanol fixed), WB
- Reacts with: Mouse, Rat, Cat, Human, Pig, Xenopus laevis, Zebrafish
- Isotype: IgG
Product nameAnti-gamma Tubulin antibody
See all gamma Tubulin primary antibodies
DescriptionRabbit polyclonal to gamma Tubulin
Tested applicationsSuitable for: IHC-P, IHC-Fr, ICC/IF, IHC (Methanol fixed), WBmore details
Species reactivityReacts with: Mouse, Rat, Cat, Human, Pig, Xenopus laevis, Zebrafish
Predicted to work with: Dog, Sea urchin
General notesStorage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab11321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/1000.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 19187565|
|IHC (Methanol fixed)||1/200. (Methanol/H2O2 fixed cells, agarose embedded): 1/200 (see PMID17329357 for further details).|
|WB||1/1000. Detects a band of approximately 48 kDa. (for whole cell extract of the human cell line HeLa and at a dilution of 1/5000 for Xenopus whole egg extract).|
FunctionTubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.
Sequence similaritiesBelongs to the tubulin family.
modificationsPhosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
Cellular localizationCytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- Gamma 1 tubulin antibody
- Gamma 2 tubulin antibody
- Gamma Tubulin 1 antibody
ICC/IF image of ab11321 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11321, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.
All lanes : Anti-gamma Tubulin antibody (ab11321) at 1/1000 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse hepatocytes whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat anti-rabbit (HRP) secondary antibody at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds
Blocking buffer 5% milk.
ab11321 at a 1/200 dilution staining gamma Tubulin in Xenopus neuroepithelial cells by Immunohistochemistry. (A) High-magnification projection of a stack of optical sections showing elongating neural epithelial cells stained for ?-tubulin (white lines indicate cell outline). (a') a-tubulin staining for cells shown in A. (B) Schematic of the closing neural tube indicating location of section shown in A (red box). (C) ?-tubulin staining in a projection of optical sections reveals centrosome-like foci at the apical surfaces of mature neuroepithelial cells. (c') Acetylated-a-tubulin staining reveals cell outlines in the ventral region of the closed Xenopus neural tube. (D) Schematic of the neural tube indicating location of section shown in C (red box). (E) Optical section showing a-tubulin in an embryo unilaterally injected with Shroom3-MO (right side is injected, left side is control). (e') ?-tubulin staining for cells shown in E.
ab11321 has been referenced in 18 publications.
- Ryan D et al. The pro- and anti-tumor roles of mesenchymal stem cells toward BRCA1-IRIS-overexpressing TNBC cells. Breast Cancer Res 21:53 (2019). PubMed: 31014367
- Park J et al. Switch-like enhancement of epithelial-mesenchymal transition by YAP through feedback regulation of WT1 and Rho-family GTPases. Nat Commun 10:2797 (2019). PubMed: 31243273
- Fokin Artem I et al. Centrosome-derived microtubule radial array, PCM-1 protein, and primary cilia formation. Protoplasma 256:1361-1373 (2019). PubMed: 31079229
- Hilfenhaus G et al. Vav3-induced cytoskeletal dynamics contribute to heterotypic properties of endothelial barriers. J Cell Biol 217:2813-2830 (2018). PubMed: 29858212
- Milunovic-Jevtic A et al. In vivo mitotic spindle scaling can be modulated by changing the levels of a single protein: the microtubule polymerase XMAP215. Mol Biol Cell 29:1311-1317 (2018). PubMed: 29851557