Anti-gamma Tubulin antibody (ab209831)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-gamma Tubulin antibody
See all gamma Tubulin primary antibodies -
Description
Rabbit polyclonal to gamma Tubulin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IHC - Wholemount, WB, IHC-Frmore details -
Species reactivity
Reacts with: Zebrafish
Predicted to work with: Mouse, Rat, Cow, Human
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Immunogen
Recombinant fragment within Zebrafish gamma Tubulin aa 2-204. The exact sequence is proprietary.
Database link: 393882 -
Positive control
- WB: Zebrafish whole cell lysate. IHC-WM: zebrafish embryos. IHC-P: zebrafish neurons and zebrafish embryos.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab209831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| IHC - Wholemount | 1/100 - 1/500. | |
| WB | 1/500 - 1/3000. | |
| IHC-Fr | Use at an assay dependent concentration. |
Target
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Function
Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. -
Sequence similarities
Belongs to the tubulin family. -
Post-translational
modificationsPhosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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Database links
- Entrez Gene: 540216 Cow
- Entrez Gene: 541101 Cow
- Entrez Gene: 27175 Human
- Entrez Gene: 7283 Human
- Entrez Gene: 103733 Mouse
- Entrez Gene: 103768 Mouse
- Entrez Gene: 252921 Rat
- Entrez Gene: 680991 Rat
see all -
Alternative names
- Gamma 1 tubulin antibody
- Gamma 2 tubulin antibody
- Gamma Tubulin 1 antibody
see all
Images
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Western blot - Anti-gamma Tubulin antibody (ab209831)Anti-gamma Tubulin antibody (ab209831) at 1/1000 dilution + Adult zebrafish whole cell lysate at 10 µg10% SDS PAGE
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IHC - Wholemount - Anti-gamma Tubulin antibody (ab209831)Whole mount immunohistochemical analysis of 1 day post-fertilization zebrafish embryo labeling gamma Tubulin with ab209831 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody (ab209831)Immunohistochemical analysis of paraffin-embedded sections of zebrafish neurons labeling gamma Tubulin with ab209831 at 1/300 dilution.
References
ab209831 has not yet been referenced specifically in any publications.
