Key features and details
- Rabbit polyclonal to gamma Tubulin - C-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human, Xenopus laevis
- Isotype: IgG
Product nameAnti-gamma Tubulin antibody - C-terminal
See all gamma Tubulin primary antibodies
DescriptionRabbit polyclonal to gamma Tubulin - C-terminal
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human, Xenopus laevis
- Xenopus mitotic extract, Xenopus sperm heads which have been incubated in a Xenopus egg mitotic extract containing nocodazole.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab188270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/200.|
|WB||1/200 - 1/1000. Predicted molecular weight: 51 kDa.|
FunctionTubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.
Sequence similaritiesBelongs to the tubulin family.
modificationsPhosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
Cellular localizationCytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- Gamma 1 tubulin antibody
- Gamma 2 tubulin antibody
- Gamma Tubulin 1 antibody
All lanes : Anti-gamma Tubulin antibody - C-terminal (ab188270) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : MCF7 cell lysates
Lysates/proteins at 20 µg per lane.
Predicted band size: 51 kDa
Immunofluorescent analysis of Xenopus sperm heads which have been incubated in a Xenopus egg mitotic extract containing nocodazole labeling gamma Tubulin with ab188270 (red) and counter stained with Dapi (blue).
Anti-gamma Tubulin antibody - C-terminal (ab188270) at 1/1000 dilution + Xenopus mitotic extract
Predicted band size: 51 kDa
ab188270 has not yet been referenced specifically in any publications.