Product nameAnti-gamma Tubulin antibody - Centrosome Marker
See all gamma Tubulin primary antibodies
DescriptionRabbit polyclonal to gamma Tubulin - Centrosome Marker
SpecificityDoes not react with alpha or beta tubulin. Immunogen sequence found in both gamma tubulin 1 and gamma tubulin 2.
Tested applicationsSuitable for: ICC/IF, IP, IHC - Wholemount, IHC-Fr, WBmore details
Species reactivityReacts with: Mouse, Human, Zebrafish
Predicted to work with: Rat, Dog, Xenopus laevis
- WB: Recombinant Human gamma Tubulin protein (denatured) (ab115710), HeLa Whole Cell Lysate, HeLa Nuclear Cell Lysate, A431 Whole Cell Lysate ICC: SK-N-SH cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab16504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IP||Use at an assay dependent concentration.|
|IHC - Wholemount||Use at an assay dependent concentration.|
|IHC-Fr||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
Abcam recommends using milk as the blocking agent.
FunctionTubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.
Sequence similaritiesBelongs to the tubulin family.
modificationsPhosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
Cellular localizationCytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- Gamma 1 tubulin antibody
- Gamma 2 tubulin antibody
- Gamma Tubulin 1 antibody
Lane 1 - 6 : gamma Tubulin antibody - Centrosome Marker (ab11317) at 1 ug/ml
Lane 1 : HeLa Whole Cell Lysate
Lane 2 : HeLa Nuclear Cell Lysate
Lane 3 : A431 Whole Cell Lysate
Lane 4 : HeLa Whole Cell Lysate with gamma Tubulin peptide (38-53) (ab17097)
Lane 5 : HeLa Nuclear Cell Lysate with gamma Tubulin peptide (38-53) (ab17097)
Lane 6 : A431 Whole Cell Lysate with gamma Tubulin peptide (38-53) (ab17097)
Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Lysates at 20 ug.
Blocking peptide at 1 ug/ml.
Performed under reducing conditions.
Observed band size : 48kD
ICC/IF image of ab16504 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab16504, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
SK-N-SH cells were fixed in 4% paraformaldehyde, permeabilized in 0.5% Triton X-100 and incubated for 1 hour with ab16504 (1/300). ab16504 staining is localized to the centrosome (red). The cells were counterstained with DAPI (blue). 100x magnification. The cells were blocked with 5% fetal bovine serum.
Paraformaldehyde-fixed mouse embryo tissue stained for gamma Tubulin (red) using ab16504 in immunohistochemical analysis.
SK-N-SH cells were fixed in 4% paraformaldehyde, permeabilized in 0.5% Triton X-100 and incubated for 1 hour with ab16504. The antibody clearly labels the centrosome (red). The cells were counterstained with DAPI (blue). The cells were blocked in 5% BSA.
ICC/IF image of ab16504 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16504, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The cause of the background staining is uncertain, although a cytokeratin exisits of a similar molecular weight and amino acid sequence to that of the immunogen used to raise the antibody.
All lanes : Anti-gamma Tubulin antibody - Centrosome Marker (ab16504) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Lane 3 : Zebrafish liver homogenate at 20 µg
Lane 4 : HeLa (Human epithelial carcinoma cell line) whole cell lysate at 20 µg
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 5 minutes
This product has been referenced in:
- Watanuki S et al. Characterization of centriole duplication in human epidermis, Bowen's disease, and squamous cell carcinoma. J Dermatol Sci 91:9-18 (2018). ICC/IF . Read more (PubMed: 29615326) »
- Scerbo P et al. Lineage commitment of embryonic cells involves MEK1-dependent clearance of pluripotency regulator Ventx2. Elife 6:N/A (2017). In situ hybridization ; Xenopus laevis . Read more (PubMed: 28654420) »