Immunocytochemistry/ Immunofluorescence abreview for Anti-gamma Tubulin antibody - Centrosome Marker

Average
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS-BSA

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti Rabbit 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594

Additional data

Additional Notes
Signal was rather diffuse, but did localize to the poles

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Submitted Dec 11 2007

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