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    gamma-tubulin-antibody-epr13872-centrosome-marker-ab180595.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microtubules Tubulin
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RecombinantRabMAb

Recombinant Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)

  • Datasheet
  • Certificate of Compliance
Reviews (1) Submit a question References (1)

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Western blot - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)
  • Flow Cytometry - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)
  • Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13872] to gamma Tubulin - Centrosome Marker
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

You may also be interested in

Protein
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Recombinant Human gamma Tubulin protein (denatured) (ab115710)
Secondary
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker
    See all gamma Tubulin primary antibodies
  • Description

    Rabbit monoclonal [EPR13872] to gamma Tubulin - Centrosome Marker
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Hela, Jurkat, A431 or 293 lysate, Human colon tissue or Jurkat cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13872
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Centrosome

Associated products

  • Alternative Versions

    • Biotin Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab197629)
    • Anti-gamma Tubulin antibody [EPR13872] - BSA and Azide free (ab250240)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab7899)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human gamma Tubulin protein (denatured) (ab115710)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab180595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC
Human
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Cow
Dog
Drosophila melanogaster
a wide range of other species
Application Abreviews Notes
WB
1/1000 - 1/2000. Detects a band of approximately 48 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Heat up to 98 °C, below boiling, and then let cool for 10-20 min.

Flow Cyt
1/140.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Notes
WB
1/1000 - 1/2000. Detects a band of approximately 48 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Heat up to 98 °C, below boiling, and then let cool for 10-20 min.

Flow Cyt
1/140.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.
  • Sequence similarities

    Belongs to the tubulin family.
  • Post-translational
    modifications

    Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome.
  • Target information above from: UniProt accession Q9NRH3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 540216 Cow
    • Entrez Gene: 541101 Cow
    • Entrez Gene: 403695 Dog
    • Entrez Gene: 490962 Dog
    • Entrez Gene: 35199 Drosophila melanogaster
    • Entrez Gene: 27175 Human
    • Entrez Gene: 7283 Human
    • Entrez Gene: 103733 Mouse
    • Entrez Gene: 103768 Mouse
    • Entrez Gene: 252921 Rat
    • Entrez Gene: 680991 Rat
    • Omim: 191135 Human
    • Omim: 605785 Human
    • SwissProt: Q0VCD2 Cow
    • SwissProt: Q32KM1 Cow
    • SwissProt: Q9GKK5 Dog
    • SwissProt: P42271 Drosophila melanogaster
    • SwissProt: P23258 Human
    • SwissProt: Q9NRH3 Human
    • SwissProt: P83887 Mouse
    • SwissProt: Q8VCK3 Mouse
    • SwissProt: P83888 Rat
    • Unigene: 469 Drosophila melanogaster
    • Unigene: 279669 Human
    • Unigene: 708059 Human
    • Unigene: 142348 Mouse
    • Unigene: 479145 Mouse
    • Unigene: 154431 Rat
    see all
  • Alternative names

    • Gamma 1 tubulin antibody
    • Gamma 2 tubulin antibody
    • Gamma Tubulin 1 antibody
    • Gamma Tubulin 2 antibody
    • Gamma tubulin complex component 1 antibody
    • Gamma-2-tubulin antibody
    • GCP 1 antibody
    • GCP-1 antibody
    • GCP1 antibody
    • MGC131994 antibody
    • TBG2_HUMAN antibody
    • TUBG antibody
    • TUBG1 antibody
    • TUBG2 antibody
    • TUBGCP1 antibody
    • Tubulin gamma 1 chain antibody
    • Tubulin gamma 2 chain antibody
    • Tubulin gamma complex-associated protein 1 antibody
    • Tubulin gamma-2 chain antibody
    • tubulin, gamma 1 antibody
    • tubulin, gamma 2 antibody
    • tubulin, gamma polypeptide antibody
    • Xgam antibody
    see all

Images

  • Western blot - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)
    Western blot - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)
    All lanes : Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595) at 1/2000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : Jurkat lysate
    Lane 3 : A431 lysate
    Lane 4 : 293 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated at 1/1000 dilution
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)

    Immunohistochemical analysis of paraffin embedded Human colon tissue labeling gamma Tubulin with ab180595 at a 1/100 dilution. Prediluted ImmunoHistoprobe HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)
    Flow Cytometry - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)

    Flow cytometry analysis of Jurkat cells using ab180595 at a 1/140 dilution (red) or a rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.

  • Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)
    Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (1)

    Publishing research using ab180595? Please let us know so that we can cite the reference in this datasheet.

    ab180595 has been referenced in 1 publication.

    • Myung Park J  et al. Beclin 1 and UVRAG Confer Protection from Radiation-Induced DNA Damage and Maintain Centrosome Stability in Colorectal Cancer Cells. PLoS One 9:e100819 (2014). WB . PubMed: 24956373

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    No
    Specification
    HeLa
    Fixative
    Methanol
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Jul 26 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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