RecombinantRabMAb

Recombinant Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)

Reviews (1) Submit a question References (1)
Western blot - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)
  • Flow Cytometry - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)
  • Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13872] to gamma Tubulin - Centrosome Marker
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker
    See all gamma Tubulin primary antibodies

  • Description

    Rabbit monoclonal [EPR13872] to gamma Tubulin - Centrosome Marker

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Hela, Jurkat, A431 or 293 lysate, Human colon tissue or Jurkat cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab180595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC
Human
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Cow
Dog
Drosophila melanogaster
a wide range of other species
Application Abreviews Notes
WB
1/1000 - 1/2000. Detects a band of approximately 48 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Heat up to 98 °C, below boiling, and then let cool for 10-20 min.
Flow Cyt
1/140.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
Notes
WB
1/1000 - 1/2000. Detects a band of approximately 48 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Heat up to 98 °C, below boiling, and then let cool for 10-20 min.
Flow Cyt
1/140.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

Images

  • Western blot - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)
    Western blot - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)
    All lanes : Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595) at 1/2000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : Jurkat lysate
    Lane 3 : A431 lysate
    Lane 4 : 293 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated at 1/1000 dilution
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)

    Immunohistochemical analysis of paraffin embedded Human colon tissue labeling gamma Tubulin with ab180595 at a 1/100 dilution. Prediluted ImmunoHistoprobe HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)
    Flow Cytometry - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)

    Flow cytometry analysis of Jurkat cells using ab180595 at a 1/140 dilution (red) or a rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.

  • Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)
    Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)

References (1)

Publishing research using ab180595? Please let us know so that we can cite the reference in this datasheet.

ab180595 has been referenced in 1 publication.

  • Myung Park J  et al. Beclin 1 and UVRAG Confer Protection from Radiation-Induced DNA Damage and Maintain Centrosome Stability in Colorectal Cancer Cells. PLoS One 9:e100819 (2014). WB . PubMed: 24956373

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Immunocytochemistry/ Immunofluorescence abreview for Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
No
Specification
HeLa
Fixative
Methanol
Read More

Dr. Kirk Mcmanus

Verified customer

Submitted Jul 26 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com