Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-gamma Tubulin antibody [EPR13872] - Centrosome Marker
See all gamma Tubulin primary antibodies -
DescriptionRabbit monoclonal [EPR13872] to gamma Tubulin - Centrosome Marker
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
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Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog, Drosophila melanogaster, a wide range of other species
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Immunogen
Synthetic peptide within Human gamma Tubulin aa 1-100. The exact sequence is proprietary.
Database link: Q9NRH3 -
Positive control
- Hela, Jurkat, A431 or 293 lysate, Human colon tissue or Jurkat cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA, PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR13872
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab180595 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/2000. Detects a band of approximately 48 kDa. | |
| IHC-P | 1/50 - 1/100. Heat up to 98 °C, below boiling, and then let cool for 10-20 min. |
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| Flow Cyt | 1/140. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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FunctionTubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.
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Sequence similaritiesBelongs to the tubulin family.
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Post-translational
modificationsPhosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome. -
Cellular localizationCytoplasm > cytoskeleton > centrosome.
- Information by UniProt
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Database links
- Entrez Gene: 540216 Cow
- Entrez Gene: 541101 Cow
- Entrez Gene: 403695 Dog
- Entrez Gene: 490962 Dog
- Entrez Gene: 35199 Drosophila melanogaster
- Entrez Gene: 27175 Human
- Entrez Gene: 7283 Human
- Entrez Gene: 103733 Mouse
see all -
Alternative names
- Gamma 1 tubulin antibody
- Gamma 2 tubulin antibody
- Gamma Tubulin 1 antibody
see all
Images
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![Western blot - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)](https://www.abcam.com/ps/products/180/ab180595/Images/ab180595-211355-ab180595wb1.jpg)
Western blot - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)All lanes : Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595) at 1/2000 dilution
Lane 1 : HeLa lysate
Lane 2 : Jurkat lysate
Lane 3 : A431 lysate
Lane 4 : 293 lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated at 1/1000 dilution -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)](https://www.abcam.com/ps/products/180/ab180595/Images/ab180595-211356-ab180595ihc1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)Immunohistochemical analysis of paraffin embedded Human colon tissue labeling gamma Tubulin with ab180595 at a 1/100 dilution. Prediluted ImmunoHistoprobe HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.
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![Flow Cytometry - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)](https://www.abcam.com/ps/products/180/ab180595/Images/ab180595-211357-ab180595fc1.jpg)
Flow Cytometry - Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (ab180595)Flow cytometry analysis of Jurkat cells using ab180595 at a 1/140 dilution (red) or a rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.
Datasheets and documents
References
This product has been referenced in:
- Myung Park J et al. Beclin 1 and UVRAG Confer Protection from Radiation-Induced DNA Damage and Maintain Centrosome Stability in Colorectal Cancer Cells. PLoS One 9:e100819 (2014). WB . Read more (PubMed: 24956373) »
