Immunocytochemistry/ Immunofluorescence abreview for Anti-gamma Tubulin antibody [EPR13872] - Centrosome Marker

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
No
Specification
HeLa
Fixative
Methanol

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/1000

Secondary antibody

Dilution
1/200

Additional data

Additional Notes
Asynchronous cells were paraformaldehyde-fixed, permeablized, immunofluorescently labelled and counterstained with DAPI. Under these conditions, cells exhibited a strong, centrosome associated staining pattern in both interphase and mitotic cells. Paraformaldehyde-fixed samples were also evaluated, but the antibody does not appear to be compatible with these conditions.

Dr. Kirk Mcmanus

Verified customer

Submitted Jul 26 2017

Sign up