Asynchronous cells were paraformaldehyde-fixed, permeablized, immunofluorescently labelled and counterstained with DAPI. Under these conditions, cells exhibited a strong, centrosome associated staining pattern in both interphase and mitotic cells. Paraformaldehyde-fixed samples were also evaluated, but the antibody does not appear to be compatible with these conditions.
Dr. Kirk Mcmanus
Submitted Jul 26 2017
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