Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13872] to gamma Tubulin - Centrosome Marker (Biotin)
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: Biotin
Product nameAnti-gamma Tubulin antibody [EPR13872] - Centrosome Marker (Biotin)
See all gamma Tubulin primary antibodies
DescriptionRabbit monoclonal [EPR13872] to gamma Tubulin - Centrosome Marker (Biotin)
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Drosophila melanogaster, a wide range of other species
Synthetic peptide within Human gamma Tubulin aa 1-100. The exact sequence is proprietary.
Database link: Q9NRH3
- IHC-P: Normal human colon tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab197629 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionTubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.
Sequence similaritiesBelongs to the tubulin family.
modificationsPhosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
Cellular localizationCytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- Gamma 1 tubulin antibody
- Gamma 2 tubulin antibody
- Gamma Tubulin 1 antibody
IHC image of gamma Tubulin staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab197629 at 1/500 dilution for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
ab197629 has been referenced in 1 publication.
- Myung Park J et al. Beclin 1 and UVRAG Confer Protection from Radiation-Induced DNA Damage and Maintain Centrosome Stability in Colorectal Cancer Cells. PLoS One 9:e100819 (2014). WB . PubMed: 24956373