RecombinantRabMAb

Recombinant Anti-gamma Tubulin antibody [EPR16793] (ab179503)

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Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
  • Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
  • Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
  • Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
  • Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
  • Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
  • Flow Cytometry - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
  • Immunoprecipitation - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
  • Anti-gamma Tubulin antibody [EPR16793] (ab179503)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16793] to gamma Tubulin
  • Suitable for: WB, ICC/IF, Flow Cyt, IHC-P, IP
  • Reacts with: Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, Fish, Monkey, Xenopus tropicalis

Overview

  • Product name

    Anti-gamma Tubulin antibody [EPR16793]
    See all gamma Tubulin primary antibodies

  • Description

    Rabbit monoclonal [EPR16793] to gamma Tubulin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IHC-P, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, Fish, Monkey, Xenopus tropicalis

  • Immunogen

    Recombinant fragment corresponding to Human gamma Tubulin aa 200 to the C-terminus.
    Database link: P23258

  • Positive control

    • WB: HeLa, MDCK, MDBK, COS-1, Jurkat, C6, RAW 264.7, PC-12, NIH/3T3, UMNSAH/DF-1 and BHK whole cell lysates; Zebrafish lysates and Xenopus (X. tropicalis) muscle lysates; Human fetal brain, fetal heart, and fetal kidney lysates; Mouse brain and spleen lysates; Rat brain, heart and kidney lysates. IHC-P: Human breast carcinoma, Mouse testis and Rat testis tissues. ICC/IF: HeLa cells. Flow: UMNSAH/DF-1 cells. IP: HeLa whole cell extract.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab179503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa).
ICC/IF 1/500.
Flow Cyt 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/30.

Target

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [EPR16793] (ab179503)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling gamma Tubulin with ab179503 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasmic and centrosome staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    1. ab179503 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

  • Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    Anti-gamma Tubulin antibody [EPR16793] (ab179503) at 1/20000 dilution + Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 48 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    All lanes : Anti-gamma Tubulin antibody [EPR16793] (ab179503) at 1/2000 dilution

    Lane 1 : Human fetal brain lysates
    Lane 2 : Human fetal heart lysates
    Lane 3 : Human fetal kidney lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 48 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    All lanes : Anti-gamma Tubulin antibody [EPR16793] (ab179503) at 1/2000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
    Lane 2 : MDCK (Canine kidney cell line) whole cell lysates
    Lane 3 : MDBK (Bovine kidney cell line) whole cell lysates
    Lane 4 : COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates
    Lane 5 : Zebrafish lysates
    Lane 6 : Xenopus (X. tropicalis) muscle lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 48 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    All lanes : Anti-gamma Tubulin antibody [EPR16793] (ab179503) at 1/20000 dilution

    Lane 1 : UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates
    Lane 2 : BHK (Hamster kidney fibroblast cells) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 48 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    All lanes : Anti-gamma Tubulin antibody [EPR16793] (ab179503) at 1/2000 dilution

    Lane 1 : Mouse brain lysates
    Lane 2 : Mouse spleen lysates
    Lane 3 : Rat brain lysates
    Lane 4 : Rat heart lysates
    Lane 5 : Rat kidney lysates
    Lane 6 : C6 (Rat glial tumor cells) whole cell lysates
    Lane 7 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
    Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 9 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 48 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR16793] (ab179503)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling gamma Tubulin with ab179503 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasmic staining on epithelial cells of mouse testis is observed. Counter stained with Hematoxylin.


    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR16793] (ab179503)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling gamma Tubulin with ab179503 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasmic staining on tumor cells of breast carcinoma is observed. Counter stained with Hematoxylin.


    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR16793] (ab179503)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling gamma Tubulin with ab179503 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on epithelial cells of rat testis is observed. Counter stained with Hematoxylin.

     

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    Flow Cytometry - Anti-gamma Tubulin antibody [EPR16793] (ab179503)

    Flow cytometric analysis of 2% paraformaldehyde-fixed UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) cells labeling gamma Tubulin with ab179503 at 1/80 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    Immunoprecipitation - Anti-gamma Tubulin antibody [EPR16793] (ab179503)

    Gamma Tubulin was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab179503 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab179503 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at 1/1500 dilution. Lane 1: HeLa whole cell extract. Lane 2: PBS instead of HeLa whole cell extract.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    Anti-gamma Tubulin antibody [EPR16793] (ab179503)

References (9)

Publishing research using ab179503? Please let us know so that we can cite the reference in this datasheet.

ab179503 has been referenced in 9 publications.

  • Fu S  et al. Glutamine Synthetase Promotes Radiation Resistance via Facilitating Nucleotide Metabolism and Subsequent DNA Damage Repair. Cell Rep 28:1136-1143.e4 (2019). PubMed: 31365859
  • Velásquez ZD  et al. Toxoplasma gondii-induced host cellular cell cycle dysregulation is linked to chromosome missegregation and cytokinesis failure in primary endothelial host cells. Sci Rep 9:12496 (2019). PubMed: 31467333
  • Vuorinen EM  et al. Depletion of nuclear import protein karyopherin alpha 7 (KPNA7) induces mitotic defects and deformation of nuclei in cancer cells. BMC Cancer 18:325 (2018). PubMed: 29580221
  • Nagane M  et al. Sulfasalazine, an inhibitor of the cystine-glutamate antiporter, reduces DNA damage repair and enhances radiosensitivity in murine B16F10 melanoma. PLoS One 13:e0195151 (2018). PubMed: 29649284
  • Cao Y  et al. The actomyosin network is influenced by NMHC IIA and regulated by CrpF46, which is involved in controlling cell migration. Exp Cell Res 373:119-131 (2018). PubMed: 30336116
  • Qu Y  et al. PHF1 is required for chromosome alignment and asymmetric division during mouse meiotic oocyte maturation. Cell Cycle 17:2447-2459 (2018). PubMed: 30382790
  • Fischer AW  et al. Intact innervation is essential for diet-induced recruitment of brown adipose tissue. Am J Physiol Endocrinol Metab N/A:N/A (2018). PubMed: 30576247
  • She ZY  et al. Minus end-directed kinesin-14 KIFC1 regulates the positioning and architecture of the Golgi apparatus. Oncotarget 8:36469-36483 (2017). PubMed: 28430595
  • Hung CY  et al. A defect in the inner kinetochore protein CENPT causes a new syndrome of severe growth failure. PLoS One 12:e0189324 (2017). PubMed: 29228025

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-gamma Tubulin antibody [EPR16793]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (oral mucosa)
Specification
oral mucosa
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Dr. Tina Yuan

Verified customer

Submitted Sep 11 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-gamma Tubulin antibody [EPR16793]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse Melanoma B16F10 cell)
Permeabilization
Yes - 0.01% TritonX100/PBS
Specification
Mouse Melanoma B16F10 cell
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Apr 19 2017

Immunohistochemistry (Frozen sections) abreview for Anti-gamma Tubulin antibody [EPR16793]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Sample
Mouse Tissue sections (adult mouse lung)
Specification
adult mouse lung
Permeabilization
Yes - 0.5% Triton X-100
Fixative
Paraformaldehyde
Read More

Dr. Hai Song

Verified customer

Submitted Oct 10 2014

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