Recombinant Anti-gamma Tubulin antibody [EPR16793] (ab179503)
![Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [EPR16793] (ab179503)](https://www.abcam.com/ps/products/179/ab179503/Images/ab179503-230503-anti-gamma-tubulin-antibody-epr16793-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [EPR16793] (ab179503)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16793] to gamma Tubulin - Centrosome Marker
- Suitable for: WB, ICC/IF, IHC-P, Flow Cyt (Intra), IP
- Reacts with: Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, Zebrafish, African green monkey, Xenopus tropicalis
Related conjugates and formulations
Overview
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Product name
Anti-gamma Tubulin antibody [EPR16793]
See all gamma Tubulin primary antibodies -
Description
Rabbit monoclonal [EPR16793] to gamma Tubulin - Centrosome Marker -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-P, Flow Cyt (Intra), IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, Zebrafish, African green monkey, Xenopus tropicalis
Predicted to work with: Fish, Monkey
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, MDCK, MDBK, COS-1, Jurkat, C6, RAW 264.7, PC-12, NIH/3T3, UMNSAH/DF-1 and BHK whole cell lysates; Zebrafish lysates and Xenopus (X. tropicalis) muscle lysates; Human fetal brain, fetal heart, and fetal kidney lysates; Mouse brain and spleen lysates; Rat brain, heart and kidney lysates. IHC-P: Human breast carcinoma, Mouse testis and Rat testis tissues. ICC/IF: HeLa cells. Flow: UMNSAH/DF-1 cells. IP: HeLa whole cell extract.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16793 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab179503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa).
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| ICC/IF | (1) |
1/500.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Flow Cyt (Intra) |
1/80.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IP |
1/30.
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| Notes |
|---|
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WB
1/2000. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa). |
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ICC/IF
1/500. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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Flow Cyt (Intra)
1/80. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IP
1/30. |
Target
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Function
Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. -
Sequence similarities
Belongs to the tubulin family. -
Post-translational
modificationsPhosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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Database links
- Entrez Gene: 540216 Cow
- Entrez Gene: 541101 Cow
- Entrez Gene: 403695 Dog
- Entrez Gene: 490962 Dog
- Entrez Gene: 27175 Human
- Entrez Gene: 7283 Human
- Entrez Gene: 103733 Mouse
- Entrez Gene: 103768 Mouse
see all -
Alternative names
- Gamma 1 tubulin antibody
- Gamma 2 tubulin antibody
- Gamma Tubulin 1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling gamma Tubulin with ab179503 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasmic and centrosome staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. ab179503 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution. -
![Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)](https://www.abcam.com/ps/products/179/ab179503/Images/ab179503-230509-anti-gamma-tubulin-antibody-epr16793-western-blot.jpg)
Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)Anti-gamma Tubulin antibody [EPR16793] (ab179503) at 1/20000 dilution + Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)](https://www.abcam.com/ps/products/179/ab179503/Images/ab179503-230508-anti-gamma-tubulin-antibody-epr16793-western-blot.jpg)
Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)All lanes : Anti-gamma Tubulin antibody [EPR16793] (ab179503) at 1/2000 dilution
Lane 1 : Human fetal brain lysates
Lane 2 : Human fetal heart lysates
Lane 3 : Human fetal kidney lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)](https://www.abcam.com/ps/products/179/ab179503/Images/ab179503-230511-anti-gamma-tubulin-antibody-epr16793-western-blot.jpg)
Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)All lanes : Anti-gamma Tubulin antibody [EPR16793] (ab179503) at 1/2000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
Lane 2 : MDCK (Canine kidney cell line) whole cell lysates
Lane 3 : MDBK (Bovine kidney cell line) whole cell lysates
Lane 4 : COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates
Lane 5 : Zebrafish lysates
Lane 6 : Xenopus (X. tropicalis) muscle lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)](https://www.abcam.com/ps/products/179/ab179503/Images/ab179503-230510-anti-gamma-tubulin-antibody-epr16793-western-blot.jpg)
Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)All lanes : Anti-gamma Tubulin antibody [EPR16793] (ab179503) at 1/20000 dilution
Lane 1 : UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates
Lane 2 : BHK (Hamster kidney fibroblast cells) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)](https://www.abcam.com/ps/products/179/ab179503/Images/ab179503-230507-anti-gamma-tubulin-antibody-epr16793-western-blot.jpg)
Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)All lanes : Anti-gamma Tubulin antibody [EPR16793] (ab179503) at 1/2000 dilution
Lane 1 : Mouse brain lysates
Lane 2 : Mouse spleen lysates
Lane 3 : Rat brain lysates
Lane 4 : Rat heart lysates
Lane 5 : Rat kidney lysates
Lane 6 : C6 (Rat glial tumor cells) whole cell lysates
Lane 7 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 9 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR16793] (ab179503)](https://www.abcam.com/ps/products/179/ab179503/Images/ab179503-230505-anti-gamma-tubulin-antibody-epr16793-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR16793] (ab179503)Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling gamma Tubulin with ab179503 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasmic staining on epithelial cells of mouse testis is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR16793] (ab179503)](https://www.abcam.com/ps/products/179/ab179503/Images/ab179503-230506-anti-gamma-tubulin-antibody-epr16793-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR16793] (ab179503)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling gamma Tubulin with ab179503 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasmic staining on tumor cells of breast carcinoma is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR16793] (ab179503)](https://www.abcam.com/ps/products/179/ab179503/Images/ab179503-230504-anti-gamma-tubulin-antibody-epr16793-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR16793] (ab179503)Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling gamma Tubulin with ab179503 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on epithelial cells of rat testis is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-gamma Tubulin antibody [EPR16793] (ab179503)](https://www.abcam.com/ps/products/179/ab179503/Images/ab179503-230502-anti-gamma-tubulin-antibody-epr16793-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-gamma Tubulin antibody [EPR16793] (ab179503)Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) cells labeling gamma Tubulin with ab179503 at 1/80 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-gamma Tubulin antibody [EPR16793] (ab179503)](https://www.abcam.com/ps/products/179/ab179503/Images/ab179503-230501-anti-gamma-tubulin-antibody-epr16793-immunoprecipitation.jpg)
Immunoprecipitation - Anti-gamma Tubulin antibody [EPR16793] (ab179503)Gamma Tubulin was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab179503 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab179503 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at 1/1500 dilution. Lane 1: HeLa whole cell extract. Lane 2: PBS instead of HeLa whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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![Anti-gamma Tubulin antibody [EPR16793] (ab179503)](https://www.abcam.com/ps/products/179/ab179503/Images/ab179503-12-benefits-of-recombinant-antibodies.png)
Anti-gamma Tubulin antibody [EPR16793] (ab179503)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (17)
ab179503 has been referenced in 17 publications.
- Sass F et al. TFEB deficiency attenuates mitochondrial degradation upon brown adipose tissue whitening at thermoneutrality. Mol Metab 47:101173 (2021). PubMed: 33516944
- Wang H et al. Reciprocal interaction between SIRT6 and APC/C regulates genomic stability. Sci Rep 11:14253 (2021). PubMed: 34244565
- Chen Y et al. HDAC8 drives spindle organization during meiotic maturation of porcine oocytes. Cell Prolif 54:e13119 (2021). PubMed: 34435400
- Zhou C et al. The cohesin stabilizer Sororin drives G2-M transition and spindle assembly in mammalian oocytes. Sci Adv 7:eabg9335 (2021). PubMed: 34559563
- Ding D et al. The microgravity induces the ciliary shortening and an increased ratio of anterograde/retrograde intraflagellar transport of osteocytes. Biochem Biophys Res Commun 530:167-172 (2020). PubMed: 32828281
