Recombinant
RabMAb

Recombinant Anti-gamma Tubulin antibody [EPR16793] (ab179503)

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Overview

  • Product name

    Anti-gamma Tubulin antibody [EPR16793]
    See all gamma Tubulin primary antibodies

  • Description

    Rabbit monoclonal [EPR16793] to gamma Tubulin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IHC-P, IP, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, Fish, Monkey, Xenopus tropicalis

  • Immunogen

    Recombinant fragment corresponding to Human gamma Tubulin aa 200 to the C-terminus.
    Database link: P23258

  • Positive control

    • WB: HeLa, MDCK, MDBK, COS-1, Jurkat, C6, RAW 264.7, PC-12, NIH/3T3, UMNSAH/DF-1 and BHK whole cell lysates; Zebrafish lysates and Xenopus (X. tropicalis) muscle lysates; Human fetal brain, fetal heart, and fetal kidney lysates; Mouse brain and spleen lysates; Rat brain, heart and kidney lysates. IHC-P: Human breast carcinoma, Mouse testis and Rat testis tissues. ICC/IF: HeLa cells. Flow: UMNSAH/DF-1 cells. IP: HeLa whole cell extract.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab179503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa).
ICC/IF 1/500.
Flow Cyt 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/30.
IHC-Fr Use at an assay dependent concentration.

Target

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [EPR16793] (ab179503)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling gamma Tubulin with ab179503 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasmic and centrosome staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    1. ab179503 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

  • Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    Anti-gamma Tubulin antibody [EPR16793] (ab179503) at 1/20000 dilution + Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 48 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    All lanes : Anti-gamma Tubulin antibody [EPR16793] (ab179503) at 1/2000 dilution

    Lane 1 : Human fetal brain lysates
    Lane 2 : Human fetal heart lysates
    Lane 3 : Human fetal kidney lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 48 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    All lanes : Anti-gamma Tubulin antibody [EPR16793] (ab179503) at 1/2000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
    Lane 2 : MDCK (Canine kidney cell line) whole cell lysates
    Lane 3 : MDBK (Bovine kidney cell line) whole cell lysates
    Lane 4 : COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates
    Lane 5 : Zebrafish lysates
    Lane 6 : Xenopus (X. tropicalis) muscle lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 48 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    All lanes : Anti-gamma Tubulin antibody [EPR16793] (ab179503) at 1/20000 dilution

    Lane 1 : UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates
    Lane 2 : BHK (Hamster kidney fibroblast cells) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 48 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    Western blot - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    All lanes : Anti-gamma Tubulin antibody [EPR16793] (ab179503) at 1/2000 dilution

    Lane 1 : Mouse brain lysates
    Lane 2 : Mouse spleen lysates
    Lane 3 : Rat brain lysates
    Lane 4 : Rat heart lysates
    Lane 5 : Rat kidney lysates
    Lane 6 : C6 (Rat glial tumor cells) whole cell lysates
    Lane 7 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
    Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 9 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 48 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR16793] (ab179503)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling gamma Tubulin with ab179503 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasmic staining on epithelial cells of mouse testis is observed. Counter stained with Hematoxylin.


    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR16793] (ab179503)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling gamma Tubulin with ab179503 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasmic staining on tumor cells of breast carcinoma is observed. Counter stained with Hematoxylin.


    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Tubulin antibody [EPR16793] (ab179503)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling gamma Tubulin with ab179503 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on epithelial cells of rat testis is observed. Counter stained with Hematoxylin.

     

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    Flow Cytometry - Anti-gamma Tubulin antibody [EPR16793] (ab179503)

    Flow cytometric analysis of 2% paraformaldehyde-fixed UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) cells labeling gamma Tubulin with ab179503 at 1/80 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-gamma Tubulin antibody [EPR16793] (ab179503)
    Immunoprecipitation - Anti-gamma Tubulin antibody [EPR16793] (ab179503)

    Gamma Tubulin was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab179503 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab179503 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at 1/1500 dilution. Lane 1: HeLa whole cell extract. Lane 2: PBS instead of HeLa whole cell extract.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

References

This product has been referenced in:

  • Fu S  et al. Glutamine Synthetase Promotes Radiation Resistance via Facilitating Nucleotide Metabolism and Subsequent DNA Damage Repair. Cell Rep 28:1136-1143.e4 (2019). Read more (PubMed: 31365859) »
  • Vuorinen EM  et al. Depletion of nuclear import protein karyopherin alpha 7 (KPNA7) induces mitotic defects and deformation of nuclei in cancer cells. BMC Cancer 18:325 (2018). Read more (PubMed: 29580221) »
See all 8 Publications for this product

Publishing research using ab179503? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-gamma Tubulin antibody [EPR16793]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (oral mucosa)
Specification
oral mucosa
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Dr. Tina Yuan

Verified customer

Submitted Sep 11 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-gamma Tubulin antibody [EPR16793]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse Melanoma B16F10 cell)
Permeabilization
Yes - 0.01% TritonX100/PBS
Specification
Mouse Melanoma B16F10 cell
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Apr 19 2017

Immunohistochemistry (Frozen sections) abreview for Anti-gamma Tubulin antibody [EPR16793]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Sample
Mouse Tissue sections (adult mouse lung)
Specification
adult mouse lung
Permeabilization
Yes - 0.5% Triton X-100
Fixative
Paraformaldehyde
Read More

Dr. Hai Song

Verified customer

Submitted Oct 10 2014

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