Product nameAnti-gamma Tubulin antibody [GTU-88]
See all gamma Tubulin primary antibodies
DescriptionMouse monoclonal [GTU-88] to gamma Tubulin
Tested applicationsSuitable for: IHC-P, ICC, ELISA, WB, IHC-FrFl, ICC/IF, IHC-FoFrmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, Xenopus laevis, Indian muntjac
Synthetic peptide conjugated to KLH, corresponding to N-terminal amino acids 38-53 of gamma Tubulin.
This product was changed from ascites to tissue culture supernatant on 18th October 2016. The following lots are from ascites and are still in stock as of 18th October 2016 (GR286332 and GR225007). Lot numbers higher than GR286332 will be from tissue culture supernatant. Please note that dilutions may need to be adjusted accordingly.
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Preservative: 0.097% Sodium azide
Concentration information loading...
Purification notesPurified from Tissue Culture Supernatant.
Our Abpromise guarantee covers the use of ab11316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. PubMed: 20890279|
|ICC||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|WB||1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
|IHC-FrFl||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 18786414|
FunctionTubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.
Sequence similaritiesBelongs to the tubulin family.
modificationsPhosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
Cellular localizationCytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- Gamma 1 tubulin antibody
- Gamma 2 tubulin antibody
- Gamma Tubulin 1 antibody
All lanes : Anti-gamma Tubulin antibody [GTU-88] (ab11316) at 1/10000 dilution
Lane 1 : HeLa cell line lysates
Lane 2 : U87 cell line lysates
Lane 3 : COS7 cell line lysates
Lane 4 : P19 cell line lysates
Lane 5 : RAT19 cell line lysates
Lane 6 : CHO cell line lysates
Lane 7 : MDBK cell line lysates
Lane 8 : MDCK cell line lysates
All lanes : Goat Anti-Mouse IgG-Peroxidase
Predicted band size: 48 kDa
ab11316 at a 1/200 dilution staining HeLa cells by Immunocytochemistry. The antibody was incubated with the cells for 30 minutes and then detected using a Cy3 conjugated Goat anti-Mouse (H+L) (green stain). The antibody recognizes gamma-tubulin located at the centrosomes in metaphase and also faintly recognizes the centrosome association during interphase.
This image is courtesy of an Abreview submitted by Kirk McManus on 2 December 2005.
Lane 1 : Anti-gamma Tubulin antibody [GTU-88] (ab11316) at 1/20000 dilution
Lane 2 : Negative control (secondary antibody only)
All lanes : Whole cell extract of chicken fibroblasts
All lanes : Alkaline Phosphatase conjugated goat anti-mouse IgG
Predicted band size: 48 kDa
Observed band size: 48 kDa
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling gamma Tubulin using ab11316 at a dilution of 1/5000. Cells were permeabilized with methanol and folowed by fixation with acetone. A Cy3-conjugated goat anti-mouse IgG was used as the secondary antibody.
This product has been referenced in:
- Madero-Pérez J et al. Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation. Mol Neurodegener 13:3 (2018). Read more (PubMed: 29357897) »
- Nekrasova O et al. Desmosomal cadherin association with Tctex-1 and cortactin-Arp2/3 drives perijunctional actin polymerization to promote keratinocyte delamination. Nat Commun 9:1053 (2018). Read more (PubMed: 29535305) »