Key features and details
- Mouse monoclonal [GTU-88] to gamma Tubulin - Centrosome Marker
- Suitable for: ICC, WB
- Reacts with: Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, Xenopus laevis, Indian muntjac
- Isotype: IgG1
Product nameAnti-gamma Tubulin antibody [GTU-88] - Centrosome Marker
See all gamma Tubulin primary antibodies
DescriptionMouse monoclonal [GTU-88] to gamma Tubulin - Centrosome Marker
Tested applicationsSuitable for: ICC, WBmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, Xenopus laevis, Indian muntjac
Synthetic peptide conjugated to KLH, corresponding to N-terminal amino acids 38-53 of gamma Tubulin.
This product was changed from ascites to tissue culture supernatant on 18th October 2016. The following lots are from ascites and are still in stock as of 18th October 2016 (GR286332 and GR225007). Lot numbers higher than GR286332 will be from tissue culture supernatant. Please note that dilutions may need to be adjusted accordingly.
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Concentration information loading...
Purification notesPurified from Tissue Culture Supernatant.
Our Abpromise guarantee covers the use of ab11316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 1 - 2 µg/ml.|
|WB||1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
FunctionTubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.
Sequence similaritiesBelongs to the tubulin family.
modificationsPhosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
Cellular localizationCytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- Gamma 1 tubulin antibody
- Gamma 2 tubulin antibody
- Gamma Tubulin 1 antibody
All lanes : Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316) at 1/10000 dilution
Lane 1 : HeLa cell line lysates
Lane 2 : U87 cell line lysates
Lane 3 : COS7 cell line lysates
Lane 4 : P19 cell line lysates
Lane 5 : RAT19 cell line lysates
Lane 6 : CHO cell line lysates
Lane 7 : MDBK cell line lysates
Lane 8 : MDCK cell line lysates
All lanes : Goat Anti-Mouse IgG-Peroxidase
Predicted band size: 48 kDa
ab11316 at a 1/200 dilution staining HeLa cells by Immunocytochemistry. The antibody was incubated with the cells for 30 minutes and then detected using a Cy3 conjugated Goat anti-Mouse (H+L) (green stain). The antibody recognizes gamma-tubulin located at the centrosomes in metaphase and also faintly recognizes the centrosome association during interphase.
This image is courtesy of an Abreview submitted by Kirk McManus on 2 December 2005.
Lane 1 : Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316) at 1/20000 dilution
Lane 2 : Negative control (secondary antibody only)
All lanes : Whole cell extract of chicken fibroblasts
All lanes : Alkaline Phosphatase conjugated goat anti-mouse IgG
Predicted band size: 48 kDa
Observed band size: 48 kDa
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling gamma Tubulin using ab11316 at a dilution of 1/5000. Cells were permeabilized with methanol and folowed by fixation with acetone. A Cy3-conjugated goat anti-mouse IgG was used as the secondary antibody.
ab11316 has been referenced in 122 publications.
- Choi YJ et al. Mutations of ADAMTS9 Cause Nephronophthisis-Related Ciliopathy. Am J Hum Genet 104:45-54 (2019). PubMed: 30609407
- Song K et al. A SEPT1-based scaffold is required for Golgi integrity and function. J Cell Sci 132:N/A (2019). PubMed: 30709970
- Kunova Bosakova M et al. Fibroblast growth factor receptor influences primary cilium length through an interaction with intestinal cell kinase. Proc Natl Acad Sci U S A N/A:N/A (2019). PubMed: 30782830
- Redmond SA et al. Development of Ependymal and Postnatal Neural Stem Cells and Their Origin from a Common Embryonic Progenitor. Cell Rep 27:429-441.e3 (2019). PubMed: 30970247
- Chiticariu E et al. CENPV Is a CYLD-Interacting Molecule Regulating Ciliary Acetylated a-Tubulin. J Invest Dermatol N/A:N/A (2019). PubMed: 31260673