Key features and details
- Rabbit polyclonal to GAMT
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human, Pig
- Isotype: IgG
Product nameAnti-GAMT antibody
See all GAMT primary antibodies
DescriptionRabbit polyclonal to GAMT
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human, Pig
Predicted to work with: Mouse
Recombinant fragment (His-T7-tag) corresponding to Human GAMT aa 64-236. (Expressed in E.coli).
VLEVGFGMAIAASKVQEAPIDEHWIIECNDGVFQRLRDWAPRQTHKVIPL KGLWEDVAPTLPDGHFDGILYDTYPLSEETWHTHQFNFIKNHAFRLLKPG GVLTYCNLTSWGELMKSKYSDITIMFEETQVPALLEAGFRRENIRTEVMA LVPPADCRYYAFPQMITPLVTKG
Database link: Q14353
- IHC-P: Human liver, breast cancer, kidney, stomach and prostate tissues. WB: Recombinant human GAMT protein; Human serum; Pig liver lysate; Rat liver and pancreas lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231972 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 26 kDa.|
Tissue specificityExpressed in liver.
PathwayAmine and polyamine biosynthesis; creatine biosynthesis; creatine from L-arginine and glycine: step 2/2.
Involvement in diseaseDefects in GAMT are the cause of guanidinoacetate methyltransferase deficiency (GAMT deficiency) [MIM:612736]. GAMT deficiency is an autosomal recessive disorder characterized by developmental delay/regression, mental retardation, severe disturbance of expressive and cognitive speech, intractable seizures and movement disturbances, severe depletion of creatine/phosphocreatine in the brain, and accumulation of guanidinoacetic acid (GAA) in brain and body fluids.
Sequence similaritiesBelongs to the RMT2 methyltransferase family.
- Information by UniProt
- CCDS2 antibody
- Epididymis secretory protein Li 20 antibody
- GAMT antibody
Paraffin-embedded human prostate tissue stained for GAMT using ab231972 at 20 µg/ml in immunohistochemical analysis. DAB staining.
All lanes : Anti-GAMT antibody (ab231972) at 1 µg/ml
Lane 1 : Human serum
Lane 2 : Pig liver lysate
Lane 3 : Rat liver lysate
Lane 4 : Rat pancreas lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 26 kDa
Anti-GAMT antibody (ab231972) at 1 µg/ml + Recombinant human GAMT protein
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 26 kDa
Paraffin-embedded human stomach tissue stained for GAMT using ab231972 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human kidney tissue stained for GAMT using ab231972 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human breast cancer tissue stained for GAMT using ab231972 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human liver tissue stained for GAMT using ab231972 at 10 µg/ml in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231972 has not yet been referenced specifically in any publications.