Overview

  • Product name

  • Description

    Rabbit polyclonal to GANAB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human GANAB aa 97-261.

  • Positive control

    • 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji and NIH-3T3 whole cell lysates; HeLa cells; SW480 xenograft; human breasr carcinoma tissue.
  • General notes

     This product was previously labelled as alpha Glucosidase II

     

Properties

Applications

Our Abpromise guarantee covers the use of ab96757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 107 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/500.

Target

  • Function

    Cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins.
  • Tissue specificity

    Detected in placenta.
  • Pathway

    Glycan metabolism; N-glycan metabolism.
  • Sequence similarities

    Belongs to the glycosyl hydrolase 31 family.
  • Cellular localization

    Endoplasmic reticulum. Golgi apparatus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha glucosidase II alpha subunit antibody
    • Alpha-glucosidase 2 antibody
    • G2AN antibody
    • GANAB antibody
    • GANAB_HUMAN antibody
    • Glu II antibody
    • Glucosidase alpha neutral AB antibody
    • Glucosidase II alpha antibody
    • Glucosidase II subunit alpha antibody
    • GluII antibody
    • KIAA0088 antibody
    • Neutral alpha glucosidase AB antibody
    • Neutral alpha glucosidase AB precursor antibody
    • Neutral alpha-glucosidase AB antibody
    see all

Images

  • Mehanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells staibed forGANAB using ab96757 at1/500 dilution (green) in ICC/IF. Alpha tubulin filaments are detected with an anti-Alpha tubulin antibody (red).

  • All lanes : Anti-GANAB antibody (ab96757) at 1/1000 dilution

    Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
    Lane 2 : GANAB knockout HeLa cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG HRP

    Predicted band size: 107 kDa

  • Paraffin-embedded human breast carcinoma tissue stained for GANAB using ab96757 at 1/500 dilution in immunohistochemical analysis.  

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

  • Anti-GANAB antibody (ab96757) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg

    Predicted band size: 107 kDa



    7.5% SDS PAGE
  • All lanes : Anti-GANAB antibody (ab96757) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate
    Lane 4 : HeLa S3 whole cell lysate
    Lane 5 : HepG2 whole cell lysate
    Lane 6 : Molt-4 whole cell lysate
    Lane 7 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 107 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of GANAB in paraffin-embedded SW480 xenograft, using ab96757 at 1/500 dilution.

  • Immunofluorescence analysis of GANAB in methanol-fixed HeLa cells, using ab96757 at 1/200 dilution (1) and costained with Hoechst 33342 (2).

References

ab96757 has not yet been referenced specifically in any publications.

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