Product nameAnti-GANAB antibody [EPR12377(B)]
See all GANAB primary antibodies
DescriptionRabbit monoclonal [EPR12377(B)] to GANAB
Tested applicationsSuitable for: Flow Cyt, IP, WBmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human GANAB aa 650-750 (Cysteine residue). The exact sequence is proprietary.
Database link: Q14697
- Human placenta, A431, HepG2, HeLa, PC-12 and NIH3T3 lysates; HeLa cells.
This product was previously labelled as alpha Glucosidase II
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab179805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/10 - 1/100.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 107 kDa.|
FunctionCleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins.
Tissue specificityDetected in placenta.
PathwayGlycan metabolism; N-glycan metabolism.
Sequence similaritiesBelongs to the glycosyl hydrolase 31 family.
Cellular localizationEndoplasmic reticulum. Golgi apparatus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Alpha glucosidase II alpha subunit antibody
- Alpha-glucosidase 2 antibody
- G2AN antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: GANAB knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: 1 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab179805 observed at 105 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab179805 was shown to specifically react with GANAB when GANAB knockout samples were used. Wild-type and GANAB knockout samples were subjected to SDS-PAGE. ab179805 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot analysis on immunoprecipitation pellet from A431 cell lysate using ab179805.
Flow cytometric analysis of permeabilized HeLa cells labeling GANAB with ab179805 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).
All lanes : Anti-GANAB antibody [EPR12377(B)] (ab179805) at 1/1000 dilution
Lane 1 : Human placenta lysate
Lane 2 : A431 lysate
Lane 3 : HepG2 lysate
Lane 4 : HeLa lysate
Lane 5 : PC-12 lysate
Lane 6 : NIH3T3 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 107 kDa
This product has been referenced in:
- Besse W et al. Isolated polycystic liver disease genes define effectors of polycystin-1 function. J Clin Invest 127:1772-1785 (2017). Read more (PubMed: 28375157) »
- Porath B et al. Mutations in GANAB, Encoding the Glucosidase IIa Subunit, Cause Autosomal-Dominant Polycystic Kidney and Liver Disease. Am J Hum Genet 98:1193-1207 (2016). Read more (PubMed: 27259053) »