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Neuroscience Cell Adhesion Proteins Membrane Proteins
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Anti-Ganglioside GD2 antibody [14.G2a] (ab68456)

  • Datasheet
  • SDS
Reviews (1)Q&A (2)References (7)

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Immunocytochemistry/ Immunofluorescence - Anti-Ganglioside GD2 antibody [14.G2a] (ab68456)

    Key features and details

    • Mouse monoclonal [14.G2a] to Ganglioside GD2
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG2a

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    Overview

    • Product name

      Anti-Ganglioside GD2 antibody [14.G2a]
      See all Ganglioside GD2 primary antibodies
    • Description

      Mouse monoclonal [14.G2a] to Ganglioside GD2
    • Host species

      Mouse
    • Tested applications

      Suitable for: ICC/IFmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Disialoganglioside GD2

    • General notes

      This product was changed from ascites to tissue culture supernatant on 30/Aug/19. Lot numbers higher than GR3295317 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.60
      Preservative: 0.1% Sodium azide
      Constituents: PBS, 1.45% Sodium chloride
    • Concentration information loading...
    • Purity

      Tissue culture supernatant
    • Clonality

      Monoclonal
    • Clone number

      14.G2a
    • Isotype

      IgG2a
    • Research areas

      • Neuroscience
      • Cell Adhesion Proteins
      • Membrane Proteins
      • Stem Cells
      • Lineage Markers
      • Ectoderm
      • Stem Cells
      • Neural Stem Cells
      • Surface Molecules
      • Cancer
      • Oncoproteins/suppressors
      • Oncoproteins
      • Other
      • Stem Cells
      • Neural Stem Cells
      • Neuron Restricted Lineage
      • Developmental Biology
      • Lineage specification
      • Ectoderm

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab68456 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF (1)
    Use at an assay dependent concentration.
    Notes
    ICC/IF
    Use at an assay dependent concentration.

    Target

    • Relevance

      Gangliosides are a group of galactose-containing cerebrosides found in the surface membranes of nerve cells. Ganglioside GD2 is a disialoganglioside expressed on tumors of neuroectodermal origin, including human neuroblastoma and melanoma, with highly restricted expression on normal tissues, principally to the cerebellum and peripheral nerves in humans. GD2 is a suitable target for immunotherapy with monoclonal antibodies or with artificial T cell receptors due to its relatively tumor specific expression.
    • Alternative names

      • Disialoganglioside GD2 antibody

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Ganglioside GD2 antibody [14.G2a] (ab68456)
      Immunocytochemistry/ Immunofluorescence - Anti-Ganglioside GD2 antibody [14.G2a] (ab68456)

      Immunocytochemistry/immunofluorescence analysis of N1E-115 (mouse neuroblastoma cell line) cells labelling Ganglioside GD2 with ab68456.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (7)

    Publishing research using ab68456? Please let us know so that we can cite the reference in this datasheet.

    ab68456 has been referenced in 7 publications.

    • Tsujimoto T  et al. An acellular bioresorbable ultra-purified alginate gel promotes intervertebral disc repair: A preclinical proof-of-concept study. EBioMedicine 37:521-534 (2018). PubMed: 30389504
    • Dewald JH  et al. TNF differentially regulates ganglioside biosynthesis and expression in breast cancer cell lines. PLoS One 13:e0196369 (2018). PubMed: 29698439
    • Feng X  et al. Expression of EMT markers and mode of surgery are prognostic in phyllodes tumors of the breast. Oncotarget 8:33365-33374 (2017). PubMed: 28380418
    • Orsi G  et al. GD2 expression in breast cancer. Oncotarget 8:31592-31600 (2017). IHC-P ; Human . PubMed: 28415563
    • Matarrese P  et al. Evidence for the involvement of GD3 ganglioside in autophagosome formation and maturation. Autophagy 10:750-65 (2014). PubMed: 24589479
    • Tarek N  et al. Unlicensed NK cells target neuroblastoma following anti-GD2 antibody treatment. J Clin Invest 122:3260-70 (2012). PubMed: 22863621
    • Battula VL  et al. Ganglioside GD2 identifies breast cancer stem cells and promotes tumorigenesis. J Clin Invest 122:2066-78 (2012). Flow Cyt ; Human . PubMed: 22585577

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Ganglioside GD2 antibody [14.G2a]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (hPSC differentiated into sympathoadrenal progenito)
    Permeabilization
    Yes - triton 0.5%
    Specification
    hPSC differentiated into sympathoadrenal progenito
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Ms. Ingrid Saldana

    Verified customer

    Submitted Apr 28 2020

    Question

    Hola ,
    ¿Qué tal? estoy interesada en inmunofluorescencia
    de doble marcaje en tejidos humanos parafinados.
    En concreto me interesarían los anticuerpos primarios, a ser posible
    monoclonales, de distintas especies para poder combinarlos, contra las
    siguientes proteínas humanas:
    * OCT4: ab18976, ab109183, ab129030
    * tenascin C: ab6346, ab3970, ab108930, ab86182
    * CD68: ab955, ab845, ab125047
    * S100A6: ab11181, ab117714, ab75676
    * ganglioside GD2: ab68456
    * Sox10: ab107532, ab122977
    * MMP9: ab76003, ab119906
    * CD163: ab111250, ab100909
    * PNMT: ab119784
    He indicado los productos que me parecían interesantes de la web, pero
    acepto sugerencias.
    Y tambíen estaría interesada en los anticuerpos secundarios marcados
    con distintos fluorocromos para anticuerpos de distintas especies.
    Necesitaría la ficha técnica y el precio de cada uno de ellos.
    Muchas gracias.
    Saludos,

    Read More

    Abcam community

    Verified customer

    Asked on May 09 2012

    Answer

    Muchas gracias por tu respuesta.

    Es importante que todos los anticuerpos que escojas para llevar a cabo IHC-P en tejido humano estén validados (y por tanto garantizados por Abcam) para funcionar en esta especie y aplicación. Esta información aparece en la hoja técnica de los productos.

    También es importante echar un vistazo a las hojas de datos de cada uno de los productos para tener en cuenta recomendaciones especificas, tales como diluciones, métodos de recuperación antigénica, y demás que pueden aparecer en ellas. A las fichas técnicas se puede acceder desde la página web, escribiendo el código del anticuerpo (ab955, por ejemplo) en la casilla azul en la parte de arriba de nuestra página web (https://www.abcam.com/).

    Desconozco las combinaciones que queréis llevar a cabo mediante inmunofluorescencia doble con los anticuerpos mencionados. En general, a la hora de hacer inmunoensayos múltiples hay que tener mucha precaución para evitar reactividad cruzada. Es importante usar anticuerpos procedentes de distintas especies si se puede, o al menos con diferentes isotipos. Una buena forma de evitar reactividad cruzada es usando secundarios de la misma especie. Además esto permite usar suero de dicha especie para bloquear.

    Te copio los links a los protocolos que tenemos en nuestra web para llevar a cabo doble inmunofluorescencia por s pudieran resultar de ayuda:

    https://www.abcam.com/index.html?pageconfig=resource&rid=11459

    https://www.abcam.com/index.html?pageconfig=resource&rid=11458

    De todas maneras si quieres que comentemos distintas posibilidades y escenarios posibles, no dudes en contactarme otra vez.

    Respecto a los secundarios, tenemos muchos anticuerpos que podrían serviros. Te aconsejo que en función de los primarios y combinaciones que vayáis a llevar a cabo, optéis por uno u otro. Para llevar a cabo la búsqueda de los secundarios desde la pagina web (https://www.abcam.com/) pincha en la pestaña “Secondary Antibodies”. Se abre una nueva ventana de “Advance Search”, en la cual se pueden elegir las categorías requeridas. La única categoría obligatoria es el isotipo del anticuerpo, pues tiene que coincidir con el del primario. El resto de categorías son opcionales, pero son muy útiles para afinar la búsqueda y elegir el anticuerpo más apropiado según la especie del primario, la conjugación deseada, la clonalidad, la aplicación en la que se va a testar, etc…

    Los precios y la disponibilidad pueden consultarse igualmente en la parte superior de la datasheet de los productos. Si necesitas un presupuesto no dudes en hacérmelo saber para que te lo envíe. Además tenemos algún descuento para pedidos superiores a 5 productos, por lo que si vais a realizar un pedido de estas características te animaría a que nos contactaras primero.

    Espero que esta información sea de ayuda. Como te digo, para cualquier aclaración o más sugerencias, estaré encantada de echar una mano.

    Read More

    Abcam Scientific Support

    Answered on May 09 2012

    Question

    What is the Immunogen seq for this ab?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 20 2011

    Answer

    Thank you for contacting Abcam.  For ab68456 the Ganglioside GD2 antibody, was produced against a human neuroblastoma cell line.  We don't have any more specific information on this immunogen.  I am sorry that I cannot provide any more information but if there is anything else I can help you with, please let me know.

    Read More

    Abcam Scientific Support

    Answered on Dec 20 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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