Overview

  • Product name
    Anti-Ganglioside GD3 antibody [R24]
    See all Ganglioside GD3 primary antibodies
  • Description
    Mouse monoclonal [R24] to Ganglioside GD3
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    SK-MEL-28 Melanoma cell line

  • Positive control
    • ICC/IF: SH-SY5Y cells. IHC-P: Human melanoma tissue. IHC-Fr: Human melanoma tisue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Affinity purified from tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    R24
  • Isotype
    IgG3
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration. PubMed: 19765188

ab91537 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr 1/40.

Target

  • Relevance
    Almost all melanomas, astrocytomas, a proportion of sarcomas, a small number of carcinomas, some nevi, as well as normal melanocytes express GD3 antigen. It is one of the most important markers for malignant melanoma. Antibodies to the GD3 ganglioside can induce partial remission of tumor growth in animals as well as in Humans via enhancement of cytotoxic and proliferative response of lymphocytes.
  • Alternative names
    • GD3 antibody

Images

  • ICC/IF image of ab11779 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11779, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Frozen section of human melanoma tissue stained for Ganglioside GD3 with ab11779 at 1/40 dilution in immunohistochemical analysis.

  • IHC image of ab11779 staining in human melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11779, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Allende ML  et al. Cerebral organoids derived from Sandhoff disease-induced pluripotent stem cells exhibit impaired neurodifferentiation. J Lipid Res 59:550-563 (2018). Read more (PubMed: 29358305) »
  • Kotlan B  et al. Challenging tumour immunological techniques that help to track cancer stem cells in malignant melanomas and other solid tumours. Contemp Oncol (Pozn) 22:41-47 (2018). Read more (PubMed: 29628793) »
See all 12 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

I am pleased to hear the customer would like to make use of our testing discount offer and test ab11779 in mouse. I have arranged the following code:

DISCOUNT CODE: #####
Expiration date: ######

This code will give you 1 free primary antibodybefore the expiration date. To redeem this offer, please submit an Abreview formouse and include this code in the Additional Comments section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

The terms and conditions applicable to this offer can be found here:

https://www.abcam.com/collaborationdiscounthttps://www.abcam.com/collaborationdiscount.

Any feedback that the customer can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving the Abreview and wish the customer luck with their research.

Read More

Question
Answer

Thank you for your enquiry. The concentration of this antibody is 400 ug/ml. I hope this information helps. Please do not hesitate to contact us if you need anything further.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up