Product nameAnti-Ganglioside GM1 antibody
See all Ganglioside GM1 primary antibodies
DescriptionRabbit polyclonal to Ganglioside GM1
SpecificityNo cross-reaction with other carbohydrate epitopes except some with asialo - GM1 which is natural background in the rabbit.
Tested applicationsSuitable for: Thin Layer Chromatography, ICC/IF, Flow Cyt, ELISAmore details
Species reactivityReacts with: African green monkey
Purified ganglioside GM1 and complete Freunds adjuvant (Bovine).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferConstituent: Whole serum
Our Abpromise guarantee covers the use of ab23943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Thin Layer Chromatography||1/1000. Phosphate buffered saline (pH 7.4) is the recommended diluent.|
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ELISA||1/10240. Optimal ELISA detection ca. 50 ng of antigen.|
RelevanceA member of the family of glycosphingolipids, monosialoanglioside acts as a receptor and antigen for cholera. GM1 ganglioside, one of the glycosphingolipids widely distributed in all tissues, occurs in highest concentrations in the central nervous system (CNS). It is primarily located in the outer surface of the mammalian cell's plasma membrane and in synaptic membranes of the CNS. GM1 ganglioside modulates a number of cell surface and receptor activities as well as neuronal differentiation and development, protein phosphorilation and synaptic function.
Cellular localizationPlasma membrane
- GM1 ganglioside antibody
- Monosialoanglioside antibody
ab23943 staining Ganglioside GM1 in African Green Monkey CV1 cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in 4% paraformaldehyde, permeabilised in 100% methanol and then blocked using 5% serum for 1 hour at room temperature. Samples were then incubated with primary antibody at 1/10 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/200 dilution. Counterstained with DAPI (1 ug/ml).
ab23943 used in Flow Cytometry.
CV1 cells (positive control) and GM95 cells (negative control) were washed in PBS, trypsinized, washed and incubated with ab23943 at a 1/50 dilution (black histograms) or isotpe control (grey histograms). The secondary antibody used was a DyLight 488 conjugated donkey anti-rabbit, used at a 1/200 dilution.
CV1 cells were positively stained while GM95 showed no staining.
This product has been referenced in:
- Wang J et al. Activation of liver X receptors inhibit LPS-induced inflammatory response in primary bovine mammary epithelial cells. Vet Immunol Immunopathol 197:87-92 (2018). WB . Read more (PubMed: 29475512) »
- Rubovitch V et al. Restoring GM1 ganglioside expression ameliorates axonal outgrowth inhibition and cognitive impairments induced by blast traumatic brain injury. Sci Rep 7:41269 (2017). Read more (PubMed: 28112258) »