Overview

  • Product name
    Anti-Ganglioside GM1 antibody
    See all Ganglioside GM1 primary antibodies
  • Description
    Rabbit polyclonal to Ganglioside GM1
  • Host species
    Rabbit
  • Specificity
    No cross-reaction with other carbohydrate epitopes except some with asialo - GM1 which is natural background in the rabbit.
  • Tested applications
    Suitable for: Thin Layer Chromatography, ICC/IF, Flow Cyt, ELISAmore details
  • Species reactivity
    Reacts with: African green monkey
  • Immunogen

    Purified ganglioside GM1 and complete Freunds adjuvant (Bovine).

Properties

Applications

Our Abpromise guarantee covers the use of ab23943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Thin Layer Chromatography 1/1000. Phosphate buffered saline (pH 7.4) is the recommended diluent.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

ELISA 1/10240. Optimal ELISA detection ca. 50 ng of antigen.

Target

  • Relevance
    A member of the family of glycosphingolipids, monosialoanglioside acts as a receptor and antigen for cholera. GM1 ganglioside, one of the glycosphingolipids widely distributed in all tissues, occurs in highest concentrations in the central nervous system (CNS). It is primarily located in the outer surface of the mammalian cell's plasma membrane and in synaptic membranes of the CNS. GM1 ganglioside modulates a number of cell surface and receptor activities as well as neuronal differentiation and development, protein phosphorilation and synaptic function.
  • Cellular localization
    Plasma membrane
  • Alternative names
    • GM1 ganglioside antibody
    • Monosialoanglioside antibody

Images

  • ab23943 staining Ganglioside GM1 in African Green Monkey CV1 cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in 4% paraformaldehyde, permeabilised in 100% methanol and then blocked using 5% serum for 1 hour at room temperature. Samples were then incubated with primary antibody at 1/10 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/200 dilution. Counterstained with DAPI (1 ug/ml).

    See Abreview

  • ab23943 used in Flow Cytometry.
    CV1 cells (positive control) and GM95 cells (negative control) were washed in PBS, trypsinized, washed and incubated with ab23943 at a 1/50 dilution (black histograms) or isotpe control (grey histograms). The secondary antibody used was a DyLight 488 conjugated donkey anti-rabbit, used at a 1/200 dilution.

    CV1 cells were positively stained while GM95 showed no staining.

    See Abreview

References

This product has been referenced in:
  • Wang J  et al. Activation of liver X receptors inhibit LPS-induced inflammatory response in primary bovine mammary epithelial cells. Vet Immunol Immunopathol 197:87-92 (2018). WB . Read more (PubMed: 29475512) »
  • Rubovitch V  et al. Restoring GM1 ganglioside expression ameliorates axonal outgrowth inhibition and cognitive impairments induced by blast traumatic brain injury. Sci Rep 7:41269 (2017). Read more (PubMed: 28112258) »
See all 5 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse Embryonic Fibroblast)
Permeabilization
No
Specification
Mouse Embryonic Fibroblast
Blocking step
Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 18 2015

Answer

The reason that species is not mentioned on the datasheet is because the target is species independent. The target is Ganglioside GM1, a molecule composed of a glycosphingolipid (ceramide and oligosaccharide) with sialic acids linked on the sugar chain. The molecular formula of this lipid is C77H139N3O31.

Therefore, as long as your samples have this molecule present, this antibody will be suitable.

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Question
Answer

Thank you for your enquiry.

I can confirm that ab23943 antibody is sold as whole antiserumt. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and serum / ascites / tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

I can confirm that for whole antiserum, concentration of antibody is known to very between 1 - 10 mg/ml. Please note this conc is not the conc of specificIgG present in soluiton.

I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Answer

Thank you very much for your reply I am pleased to hear you are considering trying GM1 antibody ab31119 in ICC-IF.  Therefore, I am pleased to provide details of the testing discount program we can offer you: I can provide a discount off a future purchase if you buy ab31119  now, test it in ICC-IF and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.  If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab31119 in ICC-IF. I will then send a discount code. This code must be issued before purchasing ab31119 so please wait for my reply before ordering. 2. Purchase ab31119 either by phone, fax, or online (www.abcam.com). 3. Test it in ICC-IF. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab31119 turns out to be unsuitable for ICC-IF, you will still receive the discount on your next purchase after your Abreview has been submitted. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. Please let me know if you have any questions about this offer and I would be happy to help you further.  

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Answer

Thank you for your enquiry. I can confirm these are different antibodies, which have been tested in different applications. Tested applications covered by our guarnatee are listed on teh online datasheets: ab23943  Anti-Ganglioside GM1 antibody is tested ICC-IF, not tested WB ab31119  Anti-Ganglioside GM1 antibody  is tested in WB but not ICC-IF   All tested application information is stated on our datasheets, and these are updated as soon as any new information is brought to our attention. In this case, as one antibody is tested in ICC-IF and the other in WB, I am sorry we would regrettably not be able to guarantee either of these antibodies for both applications. However, if you would like to test one of these  antibody in the untested application, please contact me again replying to this message prior to the purchase as you would be eligible for our testing discount program. I would be pleased to send further information. I hope this information is helpful. Please do not hesitate to contact me for any further advice or information in this regard.  

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Application
Flow Cytometry
Sample
African Green Monkey Cell (cell culture)
Specification
cell culture
Fixation
no fixation
Permeabilization
No

Mr. Nir Drayman

Verified customer

Submitted Aug 02 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Mr. Nir Drayman

Verified customer

Submitted Mar 23 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
African Green Monkey Cell (CV1 cells)
Specification
CV1 cells
Fixative
4% PFA followed by 100% Methanol
Permeabilization
Yes - 100% Methanol
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Mr. Nir Drayman

Verified customer

Submitted Jan 04 2011

Question

I am looking for an AB to analyze natural killer cells in mouse liver. The commonly used AB for FACS for Mouse NK cells NK1.1-AK (Clon PK 136) reacts with the NK cells for frozen sections for the spleen and lymph node but not for the liver. In the literature one can find that NK cells in the mouse liver can be detected with anti-asialo GM1 from WAKO (Japan). Could you tell me if the above named AB (ab23941 or ab23943) can be used in IHC of mouse liver for the NK cells and if yes, if you have this AB in stock. Maybe there is an alternative you can recommend? The second question is in regards to an AB that can detect via IHC in the mouse liver a clot (in our case to intraportal injected nude mouse liver in human Langerhansche islands for diabetes therapy). In my opinion this would be for example an Ab which reacts with Fibrin but not with Fibrinogen. As far as I know this AB only exist against human fibrin. Thanks ever so much. --------- German version -------------------------- Ich suche Antikörper zur immunhistochemischen Darstellung von natürlichen Killerzellen in der Leber von Mäusen. Der im FACS zumeist für Maus-NK-Zellen eingesetzte NK1.1-AK (Clon PK 136) reagierte zwar mit NK Zellen in Kryoschnitten von Milz und Lymphknoten, aber nicht in der Leber. In der Literatur wird angegeben, dass man NK-Zellen in der Mäuseleber mit anti-asialo GM1 von WAKO (Japan) nachweisen kann. Kann ich bitte Angaben erhalten, ob die oben genannten AK (ab23941 oder ab23943) zur immunhistologischen NK-Darstellung in der Maus geeignet sind und wenn ja, ob diese AK derzeit auch lieferbar sind. Haben Sie gegebenenfalls eine andere Empfehlung? Die zweite Frage betrifft einen Antikörper, der im histologischen Schnitt in der Mäuseleber einen Gerinnungs-Clot nachweisen kann (in userem Fall um intraportal in die nude-Maus-Leber injiziierte humane Langerhanssche Inseln zur Diabetestherapie). Aus meiner Sicht wäre das bspw. ein AK der mit Fibrin, aber nicht mit Fibrinogen reagiert. Soweit mir bekannt, gibt es einen solchen AK bisher aber nur gegen humanes Fibrin. Mit herzlichem Dank für Ihre Bemühungen Dr. Henning Jahr Medizinische Klinik und Poliklinik III Universität Giessen Rodthohl 6 D-35392 Giessen Tel: 0641-99-42846 und 42852 Fax: 0641-9942849 Henning.Jahr@innere.med.uni-giessen.de

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Answer

Thank you for your enquiry. I found your enquiry most interesting. Whilst the literature that you detail provides evidence that NK cells in the mouse liver can be detected using an anti-asialo GM1 antibody the two antibodies that you detail are yet to be tested using immunohistochemistry as an application. Therefore we could not guarantee them to work. With regards to your second question unfortunately we do not have an antibody that distinguishes between fibrin (clotted blood) and fibrinogen. The sole Fibrin monoclonal antibody in our catalogue also detects Fibrinogen. I am sorry that I cannot be of more assistance on this occasion.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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