Anti-Ganglioside GM1 antibody (ab23943)
Key features and details
- Rabbit polyclonal to Ganglioside GM1
- Suitable for: Thin Layer Chromatography, ICC/IF, ELISA
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Ganglioside GM1 antibody
See all Ganglioside GM1 primary antibodies -
Description
Rabbit polyclonal to Ganglioside GM1 -
Host species
Rabbit -
Specificity
No cross-reaction with other carbohydrate epitopes except some with asialo - GM1 which is natural background in the rabbit. -
Tested applications
Suitable for: Thin Layer Chromatography, ICC/IF, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Purified ganglioside GM1 and complete Freunds adjuvant (Bovine).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Thin Layer Chromatography |
1/1000. Phosphate buffered saline (pH 7.4) is the recommended diluent.
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ICC/IF | (3) |
Use at an assay dependent concentration.
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ELISA |
1/10240. Optimal ELISA detection ca. 50 ng of antigen.
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Notes |
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Thin Layer Chromatography
1/1000. Phosphate buffered saline (pH 7.4) is the recommended diluent. |
ICC/IF
Use at an assay dependent concentration. |
ELISA
1/10240. Optimal ELISA detection ca. 50 ng of antigen. |
Target
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Relevance
A member of the family of glycosphingolipids, monosialoanglioside acts as a receptor and antigen for cholera. GM1 ganglioside, one of the glycosphingolipids widely distributed in all tissues, occurs in highest concentrations in the central nervous system (CNS). It is primarily located in the outer surface of the mammalian cell's plasma membrane and in synaptic membranes of the CNS. GM1 ganglioside modulates a number of cell surface and receptor activities as well as neuronal differentiation and development, protein phosphorilation and synaptic function. -
Cellular localization
Plasma membrane -
Alternative names
- GM1 ganglioside antibody
- Monosialoanglioside antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Ganglioside GM1 antibody (ab23943)Image courtesy of Nir Drayman by Abreview.ab23943 staining Ganglioside GM1 in African Green Monkey CV1 cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in 4% paraformaldehyde, permeabilised in 100% methanol and then blocked using 5% serum for 1 hour at room temperature. Samples were then incubated with primary antibody at 1/10 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/200 dilution. Counterstained with DAPI (1 ug/ml).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab23943 has been referenced in 8 publications.
- Patry RT et al. Bacterial AB5 toxins inhibit the growth of gut bacteria by targeting ganglioside-like glycoconjugates. Nat Commun 10:1390 (2019). PubMed: 30918252
- Xiong X et al. Acid Sphingomyelinase regulates the localization and trafficking of palmitoylated proteins. Biol Open 8:N/A (2019). PubMed: 31142470
- Benady A et al. GM1 ganglioside prevents axonal regeneration inhibition and cognitive deficits in a mouse model of traumatic brain injury. Sci Rep 8:13340 (2018). PubMed: 30190579
- Wang J et al. Activation of liver X receptors inhibit LPS-induced inflammatory response in primary bovine mammary epithelial cells. Vet Immunol Immunopathol 197:87-92 (2018). WB . PubMed: 29475512
- Rubovitch V et al. Restoring GM1 ganglioside expression ameliorates axonal outgrowth inhibition and cognitive impairments induced by blast traumatic brain injury. Sci Rep 7:41269 (2017). PubMed: 28112258
- Tsai YT et al. GM1 Ganglioside is Involved in Epigenetic Activation Loci of Neuronal Cells. Neurochem Res 41:107-15 (2016). PubMed: 26498762
- LaRocca TJ et al. Human-specific bacterial pore-forming toxins induce programmed necrosis in erythrocytes. MBio 5:N/A (2014). PubMed: 25161188
- Banadakoppa M et al. Nitric oxide induces segregation of decay accelerating factor (DAF or CD55) from the membrane lipid-rafts and its internalization in human endometrial cells. Cell Biol Int 36:901-7 (2012). PubMed: 22574734