Overview

  • Product name
    Anti-Ganglioside GM1 antibody
    See all Ganglioside GM1 primary antibodies
  • Description
    Rabbit polyclonal to Ganglioside GM1
  • Host species
    Rabbit
  • Specificity
    Recognizes ganglioside GM1. Expresses some reactivity to asialo GM1 due to the natural background in rabbit. Does not crossreact with other carbohydrate epitopes.
  • Tested applications
    Suitable for: ICC/IF, ELISA, Thin Layer Chromatographymore details
  • Immunogen

    Other Immunogen Type corresponding to Cow Ganglioside GM1. Immunogen=Purified monosialoganglioside GM1 and complete Freund’s adjuvant.

  • General notes
    Addition of glycerol as a cryopreservative is not recommended.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituent: Whole serum
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
ELISA 1/10000.
Thin Layer Chromatography 1/1000.

Target

  • Relevance
    A member of the family of glycosphingolipids, monosialoanglioside acts as a receptor and antigen for cholera. GM1 ganglioside, one of the glycosphingolipids widely distributed in all tissues, occurs in highest concentrations in the central nervous system (CNS). It is primarily located in the outer surface of the mammalian cell's plasma membrane and in synaptic membranes of the CNS. GM1 ganglioside modulates a number of cell surface and receptor activities as well as neuronal differentiation and development, protein phosphorilation and synaptic function.
  • Cellular localization
    Plasma membrane
  • Alternative names
    • GM1 ganglioside antibody
    • Monosialoanglioside antibody

References

ab31119 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

The reason that species is not mentioned on the datasheet is because the target is species independent. The target is Ganglioside GM1, a molecule composed of a glycosphingolipid (ceramide and oligosaccharide) with sialic acids linked on the sugar chain. The molecular formula of this lipid is C77H139N3O31.

Therefore, as long as your samples have this molecule present, this antibody will be suitable.

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Answer

Thank you very much for your reply I am pleased to hear you are considering trying GM1 antibody ab31119 in ICC-IF.  Therefore, I am pleased to provide details of the testing discount program we can offer you: I can provide a discount off a future purchase if you buy ab31119  now, test it in ICC-IF and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.  If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab31119 in ICC-IF. I will then send a discount code. This code must be issued before purchasing ab31119 so please wait for my reply before ordering. 2. Purchase ab31119 either by phone, fax, or online (www.abcam.com). 3. Test it in ICC-IF. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab31119 turns out to be unsuitable for ICC-IF, you will still receive the discount on your next purchase after your Abreview has been submitted. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. Please let me know if you have any questions about this offer and I would be happy to help you further.  

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Answer

Thank you for your enquiry. I can confirm these are different antibodies, which have been tested in different applications. Tested applications covered by our guarnatee are listed on teh online datasheets: ab23943  Anti-Ganglioside GM1 antibody is tested ICC-IF, not tested WB ab31119  Anti-Ganglioside GM1 antibody  is tested in WB but not ICC-IF   All tested application information is stated on our datasheets, and these are updated as soon as any new information is brought to our attention. In this case, as one antibody is tested in ICC-IF and the other in WB, I am sorry we would regrettably not be able to guarantee either of these antibodies for both applications. However, if you would like to test one of these  antibody in the untested application, please contact me again replying to this message prior to the purchase as you would be eligible for our testing discount program. I would be pleased to send further information. I hope this information is helpful. Please do not hesitate to contact me for any further advice or information in this regard.  

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Bronchial epithelial cells)
Specification
Bronchial epithelial cells
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 24°C

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Verified customer

Submitted Mar 05 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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