Overview

  • Product name
  • Description
    Rabbit polyclonal to Gankyrin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human Gankyrin aa 12-226.
    Database link: O75832

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lyastes.
  • General notes

    Previously labelled as Proteasome 26S S10.

Properties

Applications

Our Abpromise guarantee covers the use of ab154676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 24 kDa.

Target

  • Function
    Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module.
    Acts as an proto-oncoprotein by being involved in negative regulation of tumor suppressors RB1 and p53/TP53. Overexpression is leading to phosphorylation of RB1 and proteasomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to p53/TP53 and the mono- and polyubiquitination of p53/TP53 by MDM2 suggesting a function in targeting the TP53:MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export.
  • Tissue specificity
    Overexpressed in hepatocellular carcinomas.
  • Sequence similarities
    Contains 7 ANK repeats.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 26S proteasome non-ATPase regulatory subunit 10 antibody
    • 26S proteasome regulatory subunit p28 antibody
    • Ankyrin repeat protein antibody
    • dJ889N15.2 antibody
    • Gankyrin antibody
    • Hepatocellular carcinoma-associated protein p28 II antibody
    • p28 antibody
    • p28(GANK) antibody
    • Proteasome (prosome, macropain) 26S subunit, non ATPase, 10 antibody
    • Proteasome 26S S10 antibody
    • PSD10_HUMAN antibody
    • Psmd10 antibody
    see all

Images

  • All lanes : Anti-Gankyrin antibody (ab154676) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 24 kDa



    12% SDS PAGE

References

This product has been referenced in:
  • Du S  et al. Construction of a recombinant lentivirus-mediated shRNA expression vector targeting the human PSMD10 gene and validation of RNAi efficiency in RPMI-8226 multiple myeloma cells. Oncol Rep 38:809-818 (2017). Read more (PubMed: 28677774) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab154676.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up