Recombinant Anti-Gankyrin antibody [EPR14456(2)] (ab188315)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14456(2)] to Gankyrin
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Gankyrin antibody [EPR14456(2)]
See all Gankyrin primary antibodies -
Description
Rabbit monoclonal [EPR14456(2)] to Gankyrin -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Hela, HepG2, 293, C6, Raw264.7 and K562 cell lysates. HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14456(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/110.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
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Notes |
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Flow Cyt (Intra)
1/110. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 24 kDa. |
Target
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Function
Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module.
Acts as an proto-oncoprotein by being involved in negative regulation of tumor suppressors RB1 and p53/TP53. Overexpression is leading to phosphorylation of RB1 and proteasomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to p53/TP53 and the mono- and polyubiquitination of p53/TP53 by MDM2 suggesting a function in targeting the TP53:MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export. -
Tissue specificity
Overexpressed in hepatocellular carcinomas. -
Sequence similarities
Contains 7 ANK repeats. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5716 Human
- Entrez Gene: 53380 Mouse
- Entrez Gene: 116722 Rat
- Omim: 300880 Human
- SwissProt: O75832 Human
- SwissProt: Q9Z2X2 Mouse
- SwissProt: Q9Z2X3 Rat
- Unigene: 522752 Human
see all -
Alternative names
- 26S proteasome non-ATPase regulatory subunit 10 antibody
- 26S proteasome regulatory subunit p28 antibody
- Ankyrin repeat protein antibody
see all
Images
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All lanes : Anti-Gankyrin antibody [EPR14456(2)] (ab188315) at 1/1000 dilution
Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : PSMD10 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : K562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDaLanes 1-4: Merged signal (red and green). Green - ab188315 observed at 24 kDa. Red - loading control ab8245 observed at 36 kDa.
ab188315 Anti-Gankyrin antibody [EPR14456(2)] was shown to specifically react with Gankyrin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265680 (knockout cell lysate ab258146) was used. Wild-type and Gankyrin knockout samples were subjected to SDS-PAGE. ab188315 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Intracellular flow cytometric analysis of HeLa cells fixed in 2% paraformaldehyde labeling Gankyrin with ab188315 at 1/110 dilution and Goat anti-rabbit IgG (FITC) at 1/150 dilution. Rabbit monoclonal IgG was used as an isotype control.
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All lanes : Anti-Gankyrin antibody [EPR14456(2)] (ab188315) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Raw 264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 24 kDa -
All lanes : Anti-Gankyrin antibody [EPR14456(2)] (ab188315) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 24 kDa -
All lanes : Anti-Gankyrin antibody [EPR14456(2)] (ab188315) at 1/10000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Hela cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 24 kDa
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab188315 has not yet been referenced specifically in any publications.