Anti-Gankyrin antibody [OTI3F6] (ab118009)
Key features and details
- Mouse monoclonal [OTI3F6] to Gankyrin
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Gankyrin antibody [OTI3F6]
See all Gankyrin primary antibodies -
Description
Mouse monoclonal [OTI3F6] to Gankyrin -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Gankyrin aa 1 to the C-terminus. Produced in HEK-293T cells (NP_002805).
Database link: O75832 -
Positive control
- WB: HEK-293T cell lysate transfected with the pCMV6-ENTRY Gankyrin cDNA; HepG2 cell extract. Flow Cyt (Intra): HeLa and Jurkat cells.
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General notes
Clone OTI3F6 (formerly 3F6).
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol, PBS -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI3F6 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab118009 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/100.
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WB |
1/2000. Predicted molecular weight: 24 kDa.
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Notes |
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Flow Cyt (Intra)
1/100. |
WB
1/2000. Predicted molecular weight: 24 kDa. |
Target
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Function
Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module.
Acts as an proto-oncoprotein by being involved in negative regulation of tumor suppressors RB1 and p53/TP53. Overexpression is leading to phosphorylation of RB1 and proteasomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to p53/TP53 and the mono- and polyubiquitination of p53/TP53 by MDM2 suggesting a function in targeting the TP53:MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export. -
Tissue specificity
Overexpressed in hepatocellular carcinomas. -
Sequence similarities
Contains 7 ANK repeats. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5716 Human
- Omim: 300880 Human
- SwissProt: O75832 Human
- Unigene: 522752 Human
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Alternative names
- 26S proteasome non-ATPase regulatory subunit 10 antibody
- 26S proteasome regulatory subunit p28 antibody
- Ankyrin repeat protein antibody
see all
Images
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All lanes : Anti-Gankyrin antibody [OTI3F6] (ab118009) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA for 48 hrs
Lane 2 : HEK-293T lysate transfected with pCMV6-ENTRY Proteasome 26S S10 cDNA for 48 hrs
Lysates/proteins at 5 µg per lane.
Predicted band size: 24 kDa -
All lanes : Anti-Gankyrin antibody [OTI3F6] (ab118009) at 1/2000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : HT-29 (human colorectal adenocarcinoma cell line) cell extract
Lane 4 : A549 (human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
Lane 7 : MDCK (canine kidney cell line) extract
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract
Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 24 kDa -
Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Gankyrin (red) using ab118009 at 1/100 dilution, compared to a nonspecific negative control antibody (blue).
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Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling Gankyrin (red) using ab118009 at 1/100 dilution, compared to a nonspecific negative control antibody (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab118009 has not yet been referenced specifically in any publications.