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    gant-61-gli1-and-2-inhibitor-ab120904.pdf

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Stem Cells Signaling Pathways Hedgehog Surface Molecules
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GANT 61, GLI1 and 2 inhibitor (ab120904)

  • Datasheet
  • SDS
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Chemical Structure - GANT 61, GLI1 and 2 inhibitor (ab120904)
  • Functional Studies - GANT 61, GLI1 and 2 inhibitor (ab120904)

Key features and details

  • Selective GLI1 and 2 inhibitor
  • CAS Number: 500579-04-4
  • Purity: > 98%
  • Soluble in DMSO to 50 mM and in ethanol to 100 mM
  • Form / State: Solid
  • Source: Synthetic

Overview

  • Product name

    GANT 61, GLI1 and 2 inhibitor
  • Description

    Selective GLI1 and 2 inhibitor
  • Alternative names

    • NSC 136476
  • Biological description

    Selective GLI1 and 2 inhibitor (IC50 = 5 μM). Blocks the hedgehog/smoothened signaling pathway. Anticancer activity in vitro and in vivo.
  • Purity

    > 98%
  • CAS Number

    500579-04-4
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    2,2'-[[Dihydro-2-(4-pyridinyl)-1,3(2H,4H)-pyrimidinediyl]bis(methylene)]bis(N,N-dimethylbenzenamine)
  • Molecular weight

    429.61
  • Molecular formula

    C27H35N5
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO to 50 mM and in ethanol to 100 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    CN(C)C1=CC=CC=C1CN2CCCN(CC3=CC=CC=C3N(C)C)C2C4=CC=NC=C4
  • Source

    Synthetic

  • Research areas

    • Stem Cells
    • Signaling Pathways
    • Hedgehog
    • Surface Molecules
    • Biochemicals
    • Chemical Type
    • Biochemicals
    • Biochemicals
    • Pharmacology
    • Receptors & Transporters
    • Smoothened
    • Biochemicals
    • Pharmacology
    • Signaling
    • Signal transduction
    • Hedgehog signaling
    • Inhibitors
    • Biochemicals
    • Research Area
    • Heart disease
    • Signaling
    • DNA, RNA & protein synthesis
    • Inhibitors
    • Biochemicals
    • Research Area
    • Pain & inflammation
    • Signaling
    • DNA, RNA & protein synthesis
    • Inhibitors
    • Biochemicals
    • Pharmacology
    • Signaling
    • Signal transduction
    • Hedgehog signaling
    • Smoothened
    • Biochemicals
    • Research Area
    • Alzheimer's Disease
    • Signaling
    • Signal transduction
    • Hedgehog signaling
    • Inhibitors
    • Biochemicals
    • Research Area
    • Alzheimer's Disease
    • Signaling
    • DNA, RNA & protein synthesis
    • Inhibitors
    • Biochemicals
    • Research Area
    • Alzheimer's Disease
    • Signaling
    • Signal transduction
    • Hedgehog signaling
    • Smoothened
    • Biochemicals
    • Research Area
    • Diabetes
    • Signaling
    • Signal transduction
    • Hedgehog signaling
    • Inhibitors
    • Biochemicals
    • Research Area
    • Diabetes
    • Signaling
    • DNA, RNA & protein synthesis
    • Inhibitors
    • Biochemicals
    • Research Area
    • Diabetes
    • Signaling
    • Signal transduction
    • Hedgehog signaling
    • Smoothened
    • Biochemicals
    • Research Area
    • Hypertension
    • Signaling
    • Signal transduction
    • Hedgehog signaling
    • Inhibitors
    • Biochemicals
    • Research Area
    • Hypertension
    • Signaling
    • DNA, RNA & protein synthesis
    • Inhibitors
    • Biochemicals
    • Research Area
    • Hypertension
    • Signaling
    • Signal transduction
    • Hedgehog signaling
    • Smoothened
    • Biochemicals
    • Research Area
    • Obesity
    • Signaling
    • Signal transduction
    • Hedgehog signaling
    • Inhibitors
    • Biochemicals
    • Research Area
    • Obesity
    • Signaling
    • DNA, RNA & protein synthesis
    • Inhibitors
    • Biochemicals
    • Research Area
    • Obesity
    • Signaling
    • Signal transduction
    • Hedgehog signaling
    • Smoothened
    • Biochemicals
    • Research Area
    • Respiratory disease
    • Signaling
    • DNA, RNA & protein synthesis
    • Inhibitors
    • Biochemicals
    • Research Area
    • Stroke
    • Signaling
    • Signal transduction
    • Hedgehog signaling
    • Inhibitors
    • Biochemicals
    • Research Area
    • Stroke
    • Signaling
    • DNA, RNA & protein synthesis
    • Inhibitors
    • Biochemicals
    • Research Area
    • Stroke
    • Signaling
    • Signal transduction
    • Hedgehog signaling
    • Smoothened
    • Biochemicals
    • Pharmacology
    • Signaling
    • DNA, RNA & protein synthesis
    • Transcription factors
    • Biochemicals
    • Pharmacology
    • Signaling
    • Stem Cells
    • Hedgehog signaling
    • Biochemicals
    • Research Area
    • Cancer
    • DNA, RNA & protein synthesis
    • Transcription factors
    • Biochemicals
    • Research Area
    • Cancer
    • Signal transduction
    • Hedgehog signaling
    • Inhibitors
    • Biochemicals
    • Research Area
    • Cancer
    • Signal transduction
    • Hedgehog signaling
    • Smoothened
    • Biochemicals
    • Research Area
    • Stem Cells
    • Hedgehog signaling

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab120904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies
Use at an assay dependent concentration.
Notes
Functional Studies
Use at an assay dependent concentration.

Images

  • Chemical Structure - GANT 61, GLI1 and 2 inhibitor (ab120904)
    Chemical Structure - GANT 61, GLI1 and 2 inhibitor (ab120904)
    2D chemical structure image of ab120904, GANT 61, GLI1 and 2 inhibitor
  • Functional Studies - GANT 61, GLI1 and 2 inhibitor (ab120904)
    Functional Studies - GANT 61, GLI1 and 2 inhibitor (ab120904)
    ab11169 staining MDC1 in HCT116 cells treated with GANT 61 (ab120904), by ICC/IF. Increase in MDC1 expression correlates with increased concentration of GANT 61, as described in literature.
    The cells were incubated at 37°C for 4 hours in media containing different concentrations of ab120904 (GANT 61) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11169 (1/200 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (6)

Publishing research using ab120904? Please let us know so that we can cite the reference in this datasheet.

ab120904 has been referenced in 6 publications.

  • Zeng C  et al. Hedgehog signaling activation required for glypican-6-mediated regulation of invasion, migration, and epithelial-mesenchymal transition of gastric cancer cells. Biosci Rep 40:N/A (2020). PubMed: 32478377
  • Qi H  et al. Inactivation of Nell-1 in Chondrocytes Significantly Impedes Appendicular Skeletogenesis. J Bone Miner Res 34:533-546 (2019). PubMed: 30352124
  • Dong H  et al. GLI1 activation by non-classical pathway integrin avß3/ERK1/2 maintains stem cell-like phenotype of multicellular aggregates in gastric cancer peritoneal metastasis. Cell Death Dis 10:574 (2019). PubMed: 31366904
  • Shao JB  et al. The mechanism of epithelial-mesenchymal transition induced by TGF-ß1 in neuroblastoma cells. Int J Oncol 50:1623-1633 (2017). PubMed: 28393230
  • Zhang C  et al. Gli1 promotes colorectal cancer metastasis in a Foxm1-dependent manner by activating EMT and PI3K-AKT signaling. Oncotarget 7:86134-86147 (2016). Blocking ; Human . PubMed: 27863385
  • Xu Y  et al. Inhibition of the Hedgehog pathway induces autophagy in pancreatic ductal adenocarcinoma cells. Oncol Rep 31:707-12 (2014). PubMed: 24297612

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