GANT 61, GLI1 and 2 inhibitor (ab120904)
- Datasheet
- References (2)
Overview
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Product nameGANT 61, GLI1 and 2 inhibitor
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DescriptionSelective GLI1 and 2 inhibitor
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Alternative names
- NSC 136476
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Biological descriptionSelective GLI1 and 2 inhibitor (IC50 = 5 μM). Blocks the hedgehog/smoothened signaling pathway. Anticancer activity in vitro and in vivo.
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Purity> 98%
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CAS Number500579-04-4
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Chemical structure
Properties
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Chemical name2,2'-[[Dihydro-2-(4-pyridinyl)-1,3(2H,4H)-pyrimidinediyl]bis(methylene)]bis(N,N-dimethylbenzenamine)
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Molecular weight429.61
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Molecular formulaC27H35N5
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Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
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Solubility overviewSoluble in DMSO to 50 mM and in ethanol to 100 mM
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Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILESCN(C)C1=CC=CC=C1CN2CCCN(CC3=CC=CC=C3N(C)C)C2C4=CC=NC=C4
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Source
Synthetic
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Research areas
Applications
Our Abpromise guarantee covers the use of ab120904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies | Use at an assay dependent concentration. |
Images
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Functional Studies - GANT 61, GLI1 and 2 inhibitor (ab120904)ab11169 staining MDC1 in HCT116 cells treated with GANT 61 (ab120904), by ICC/IF. Increase in MDC1 expression correlates with increased concentration of GANT 61, as described in literature.
The cells were incubated at 37°C for 4 hours in media containing different concentrations of ab120904 (GANT 61) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11169 (1/200 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
References
This product has been referenced in:
- Pan D et al. Gli inhibitor GANT61 causes apoptosis in myeloid leukemia cells and acts in synergy with rapamycin. Leuk Res 36:742-8 (2012). Read more (PubMed: 22398221) »
- Barsoum I & Yao HH Redundant and differential roles of transcription factors Gli1 and Gli2 in the development of mouse fetal Leydig cells. Biol Reprod 84:894-9 (2011). Read more (PubMed: 21209421) »
- Lauth M et al. Inhibition of GLI-mediated transcription and tumor cell growth by small-molecule antagonists. Proc Natl Acad Sci U S A 104:8455-60 (2007). Read more (PubMed: 17494766) »
