Overview

  • Product name

    Anti-GAP43 antibody [EP890Y] (HRP)
    See all GAP43 primary antibodies
  • Description

    Rabbit monoclonal [EP890Y] to GAP43 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human GAP43 (C terminal). The exact sequence is proprietary.

  • Positive control

    • IHC-P: Normal human cerebellum tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab196325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons.
  • Sequence similarities

    Belongs to the neuromodulin family.
    Contains 1 IQ domain.
  • Post-translational
    modifications

    Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
  • Cellular localization

    Cell membrane. Cell projection > growth cone membrane. Cell junction > synapse. Cytoplasmic surface of growth cone and synaptic plasma membranes.
  • Information by UniProt
  • Database links

  • Alternative names

    • Axonal membrane protein GAP 43 antibody
    • Axonal membrane protein GAP-43 antibody
    • B 50 antibody
    • Calmodulin binding protein P 57 antibody
    • F1 antibody
    • GAP 43 antibody
    • GAP43 antibody
    • Growth Associated Protein 43 antibody
    • Growth-associated protein 43 antibody
    • Nerve Growth Related Peptide antibody
    • Nerve growth related peptide GAP43 antibody
    • NEUM_HUMAN antibody
    • Neural phosphoprotein B 50 antibody
    • Neural phosphoprotein B-50 antibody
    • Neuromodulin antibody
    • Neuron growth associated protein 43 antibody
    • PP46 antibody
    • Protein F1 antibody
    • QtrA-11580 antibody
    • QtrA-13071 antibody
    see all

Images

  • IHC image of GAP43 staining in a section of formalin-fixed paraffin-embedded normal human cerebellum, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab196325 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

ab196325 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab196325.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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