Recombinant
RabMAb

Recombinant Anti-GAP43 antibody [EP890Y] (Phycoerythrin) (ab208745)

Overview

  • Product name

    Anti-GAP43 antibody [EP890Y] (Phycoerythrin)
    See all GAP43 primary antibodies
  • Description

    Rabbit monoclonal [EP890Y] to GAP43 (Phycoerythrin)
  • Host species

    Rabbit
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity


    Predicted to work with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human GAP43 aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: P17677

  • Positive control

    • Flow Cyt: SH-SY5Y cells ICC/IF: U87MG cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab208745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/2500.

ab209478 - Rabbit monoclonal IgG (Phycoerythrin), is suitable for use as an isotype control with this antibody.

ICC/IF 1/100.

This product gave a positive signal in u87mg cells fixed with 100% methanol (5 min)

Target

  • Function

    This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons.
  • Sequence similarities

    Belongs to the neuromodulin family.
    Contains 1 IQ domain.
  • Post-translational
    modifications

    Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
  • Cellular localization

    Cell membrane. Cell projection > growth cone membrane. Cell junction > synapse. Cytoplasmic surface of growth cone and synaptic plasma membranes.
  • Information by UniProt
  • Database links

  • Alternative names

    • Axonal membrane protein GAP 43 antibody
    • Axonal membrane protein GAP-43 antibody
    • B 50 antibody
    • Calmodulin binding protein P 57 antibody
    • F1 antibody
    • GAP 43 antibody
    • GAP43 antibody
    • Growth Associated Protein 43 antibody
    • Growth-associated protein 43 antibody
    • Nerve Growth Related Peptide antibody
    • Nerve growth related peptide GAP43 antibody
    • NEUM_HUMAN antibody
    • Neural phosphoprotein B 50 antibody
    • Neural phosphoprotein B-50 antibody
    • Neuromodulin antibody
    • Neuron growth associated protein 43 antibody
    • PP46 antibody
    • Protein F1 antibody
    • QtrA-11580 antibody
    • QtrA-13071 antibody
    see all

Images

  • Overlay histogram showing SH-SY5Y cells stained with ab208745 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab208745, 1/2500 dilution) for 30 min at 22ºC.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20 mW Solid State Blue Laser (488nm) and 585/40 bandpass filter.

    This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

  • ab208745 staining GAP43 in u87mg cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab208745 at 1/100 dilution (Pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab208745 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab208745.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up