Product nameAnti-GAP43 antibody [EP928Y]
See all GAP43 primary antibodies
DescriptionRabbit monoclonal [EP928Y] to GAP43
Specificityab75843 detects both phosphorylated and nonphosphorylated GAP43.
Tested applicationsSuitable for: WB, ICCmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human GAP43 (phospho S41). The exact sequence is proprietary.
- Human brain lysate.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab75843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50000 - 1/100000. Detects a band of approximately 48 kDa (predicted molecular weight: 25 kDa).|
|ICC||1/100 - 1/250.|
FunctionThis protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons.
Sequence similaritiesBelongs to the neuromodulin family.
Contains 1 IQ domain.
modificationsPhosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
Cellular localizationCell membrane. Cell projection > growth cone membrane. Cell junction > synapse. Cytoplasmic surface of growth cone and synaptic plasma membranes.
- Information by UniProt
- Axonal membrane protein GAP 43 antibody
- Axonal membrane protein GAP-43 antibody
- B 50 antibody
Anti-GAP43 antibody [EP928Y] (ab75843) at 1/100000 dilution + human brain lysate at 10 µg
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Zhang G et al. Combined application of Rho-ROCKII and GSK-3ß inhibitors exerts an improved protective effect on axonal regeneration in rats with spinal cord injury. Mol Med Rep 14:5180-5188 (2016). Read more (PubMed: 27840930) »