Key features and details
- Rabbit monoclonal [EP928Y] to GAP43
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-GAP43 antibody [EP928Y]
See all GAP43 primary antibodies
DescriptionRabbit monoclonal [EP928Y] to GAP43
Specificityab75843 detects both phosphorylated and nonphosphorylated GAP43.
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human GAP43 (phospho S41). The exact sequence is proprietary.
- Human brain lysate.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab75843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50000 - 1/100000. Detects a band of approximately 48 kDa (predicted molecular weight: 25 kDa).|
FunctionThis protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons.
Sequence similaritiesBelongs to the neuromodulin family.
Contains 1 IQ domain.
modificationsPhosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
Cellular localizationCell membrane. Cell projection > growth cone membrane. Cell junction > synapse. Cytoplasmic surface of growth cone and synaptic plasma membranes.
- Information by UniProt
- Axonal membrane protein GAP 43 antibody
- Axonal membrane protein GAP-43 antibody
- B 50 antibody
Anti-GAP43 antibody [EP928Y] (ab75843) at 1/100000 dilution + human brain lysate at 10 µg
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
ab75843 has been referenced in 1 publication.
- Zhang G et al. Combined application of Rho-ROCKII and GSK-3ß inhibitors exerts an improved protective effect on axonal regeneration in rats with spinal cord injury. Mol Med Rep 14:5180-5188 (2016). PubMed: 27840930