Overview

  • Product name

    Anti-GAP43 antibody [EPR5031]
    See all GAP43 primary antibodies
  • Description

    Rabbit monoclonal [EPR5031] to GAP43
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human GAP43 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • SH-SY5Y and Human brain lysates; Human glioma tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 24 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons.
    • Sequence similarities

      Belongs to the neuromodulin family.
      Contains 1 IQ domain.
    • Post-translational
      modifications

      Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
    • Cellular localization

      Cell membrane. Cell projection > growth cone membrane. Cell junction > synapse. Cytoplasmic surface of growth cone and synaptic plasma membranes.
    • Information by UniProt
    • Database links

    • Alternative names

      • Axonal membrane protein GAP 43 antibody
      • Axonal membrane protein GAP-43 antibody
      • B 50 antibody
      • Calmodulin binding protein P 57 antibody
      • F1 antibody
      • GAP 43 antibody
      • GAP43 antibody
      • Growth Associated Protein 43 antibody
      • Growth-associated protein 43 antibody
      • Nerve Growth Related Peptide antibody
      • Nerve growth related peptide GAP43 antibody
      • NEUM_HUMAN antibody
      • Neural phosphoprotein B 50 antibody
      • Neural phosphoprotein B-50 antibody
      • Neuromodulin antibody
      • Neuron growth associated protein 43 antibody
      • PP46 antibody
      • Protein F1 antibody
      • QtrA-11580 antibody
      • QtrA-13071 antibody
      see all

    Images

    • All lanes : Anti-GAP43 antibody [EPR5031] (ab134075) at 1/1000 dilution

      Lane 1 : SH-SY5Y lysate
      Lane 2 : Human brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-rabbit at 1/2000 dilution

      Predicted band size: 24 kDa

    • Immunohistochemical analysis of paraffin embedded Human glioma tissue labelling GAP43 with ab134075 at a dilution of 1/250.

    References

    ab134075 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134075.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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