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Neuroscience Cell Type Marker Neuron marker Growth Cone
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Anti-GAP43 antibody - Neuronal Marker (ab12274)

  • Datasheet
  • SDS
Reviews (5)Q&A (1)References (18)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAP43 antibody - Neuronal Marker (ab12274)

    Key features and details

    • Rabbit polyclonal to GAP43 - Neuronal Marker
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

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    TMRE-Mitochondrial Membrane Potential Assay Kit (ab113852)
    Protein
    Product image
    Recombinant Human GAP43 protein (ab114188)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-GAP43 antibody - Neuronal Marker
      See all GAP43 primary antibodies
    • Description

      Rabbit polyclonal to GAP43 - Neuronal Marker
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      IHC-P
      Human
      See all applications and species data
    • Immunogen

      GAP43 purified from cat brain.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.01% Sodium azide
    • Concentration information loading...
    • Purity

      Whole antiserum
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Growth Cone
      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Synapse marker
      • Neuroscience
      • Neurology process
      • Growth and Development
      • Axonal Guidance Proteins

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human GAP43 protein (ab114188)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab12274 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IHC-P
    Human
    Application Abreviews Notes
    IHC-P (2)
    1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    Notes
    IHC-P
    1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    Target

    • Function

      This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons.
    • Sequence similarities

      Belongs to the neuromodulin family.
      Contains 1 IQ domain.
    • Post-translational
      modifications

      Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
    • Cellular localization

      Cell membrane. Cell projection > growth cone membrane. Cell junction > synapse. Cytoplasmic surface of growth cone and synaptic plasma membranes.
    • Target information above from: UniProt accession P17677 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2596 Human
      • GenBank: NP_002036 Human
      • Omim: 162060 Human
      • SwissProt: P17677 Human
      • Unigene: 134974 Human
      • Alternative names

        • Axonal membrane protein GAP 43 antibody
        • Axonal membrane protein GAP-43 antibody
        • B 50 antibody
        • Calmodulin binding protein P 57 antibody
        • F1 antibody
        • GAP 43 antibody
        • GAP43 antibody
        • Growth Associated Protein 43 antibody
        • Growth-associated protein 43 antibody
        • Nerve Growth Related Peptide antibody
        • Nerve growth related peptide GAP43 antibody
        • NEUM_HUMAN antibody
        • Neural phosphoprotein B 50 antibody
        • Neural phosphoprotein B-50 antibody
        • Neuromodulin antibody
        • Neuron growth associated protein 43 antibody
        • PP46 antibody
        • Protein F1 antibody
        • QtrA-11580 antibody
        • QtrA-13071 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAP43 antibody - Neuronal Marker (ab12274)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAP43 antibody - Neuronal Marker (ab12274)
        Ab12274 staining human neuropil. Staining is localized to the cytoplasm and membrane.
        Left panel: with primary antibody diluted 1:2000. Right panel: isotype control.
        Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, citrate pH 6.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

      Protocols

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (18)

      Publishing research using ab12274? Please let us know so that we can cite the reference in this datasheet.

      ab12274 has been referenced in 18 publications.

      • Gu J  et al. Neurobehavioral effects of bisphenol S exposure in early life stages of zebrafish larvae (Danio rerio). Chemosphere 217:629-635 (2019). PubMed: 30447611
      • Hu T  et al. Electro-acupuncture-induced neuroprotection is associated with activation of the IGF-1/PI3K/Akt pathway following adjacent dorsal root ganglionectomies in rats. Int J Mol Med 43:807-820 (2019). PubMed: 30569108
      • Rice FL  et al. The evolution and multi-molecular properties of NF1 cutaneous neurofibromas originating from C-fiber sensory endings and terminal Schwann cells at normal sites of sensory terminations in the skin. PLoS One 14:e0216527 (2019). PubMed: 31107888
      • Zhao X  et al. Risk-Associated Long Noncoding RNA FOXD3-AS1 Inhibits Neuroblastoma Progression by Repressing PARP1-Mediated Activation of CTCF. Mol Ther 26:755-773 (2018). PubMed: 29398485
      • Pohland M  et al. MH84 improves mitochondrial dysfunction in a mouse model of early Alzheimer's disease. Alzheimers Res Ther 10:18 (2018). PubMed: 29433569
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-6 of 6 Abreviews or Q&A

      Question

      Bitte das Angebot an diese Email-Adresse schicken.

      Read More

      Abcam community

      Verified customer

      Asked on Mar 09 2012

      Answer

      Vielen Dank für Ihren netten Anruf gestern und die Publikation, die Sie mir geschickt haben.

      Wie versprochen, habe ich mir die Abbildung 1 angesehen und daraufhin unsere GAP43- Antikörper danach durchsucht, welche für Gefrierschnitte und Paraffinschnitte (IHC-Fr, IHC-P) des murinen olfaktorischen Systems geeignet sein könnten.

      Folgende Antikörper könnten vielleicht für Sie geeignet sein:

      Ab12274: rabbit polyclonal, whole antiserum, 410 EUR, 100μl

      Click here (or use the following: https://www.abcam.com/GAP43-antibody-Neuronal-Marker-ab12274.html).

      Ab 75057: rabbit polyclonal, whole antiserum, 370 EUR, 100 μl

      Click here (or use the following: https://www.abcam.com/GAP43-antibody-ab75057.html).

      Ab50608: mouse monoclonal, Ascites, 370 EUR, 100 μl

      Click here (or use the following: https://www.abcam.com/GAP43-antibody-GAP-7B10-ab50608.html).

      Ab82268: mouse monoclonal, Protein A purified, 370 EUR, 100 μg

      Click here (or use the following: https://www.abcam.com/GAP43-antibody-GAP-8A12-ab82268.html).

      Ab117265: mouse monoclonal, Protein A purified, 370 EUR, 50 μg

      Click here (or use the following: https://www.abcam.com/GAP43-antibody-ab117265.html).

      Weitere Details finden Sie auf dem jeweiligen Datenblatt.

      Wie am Telefon schon erwähnt, möchte ich Ihnen versichern, dass alle unsere Produkte unseren strikten Qualitätskontrollen unterliegen und wir daher für alle Produkte eine Garantie übernehmen: Falls sich herausstellt, dass der Antikörper mit einem optimierten Protokoll nicht so funktioniert, wie auf dem Datenblatt beschrieben und Sie sich innerhalb von sechs Monaten mit Ihrem Problem an uns wenden, werden wir Ihnen gerne einen Ersatz oder eine Gutschrift schicken (www.abcam.com/Abpromise).

      Gerne sende ich Ihnen auch die Informationen zu unseren aktuellen Rabattprogrammen:

      Außerdem sind alle genannten Antikörper in unserem 100%-Rabattprogramm für Abbildungen: Da wir derzeit keine Abbildungen für die Anwendung dieser Antikörper an Gefrierschnitten vorliegen haben, können Sie einen kostenlosen primären Antikörper Ihrer Wahl von uns bekommen, falls Sie uns eine Abbildung Ihrer Ergebnisse in der IHC-Fr zukommen lassen.

      Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

      1.) Bestätigen Sie mir bitte, welchen Antikörper Sie kaufen möchten und dass Sie bereit sind, uns eine Abbildung Ihrer Ergebnisse zukommen zu lassen.

      2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

      3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

      4.) Testen Sie den Antikörper in der IHC-Fr.

      5.) Senden Sie uns die Abbildung Ihres Ergebnisses mittels eines Abreviews zu und notieren Sie den Discount Code in dem Feld "additional notes". Unter der folgenden URL können Sie mehr über unser Abreview System erfahren: https://www.abcam.com/abreviews

      6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können einen anderen primären Antikörper bei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode und die Bestellnummer bereit). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

      Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

      Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen: https://www.abcam.com/collaborationdiscount

      Des Weiteren möchte ich Ihnen noch einmal die Information zu unseren EasyLink-Kits senden, mit denen Sie schnell und einfach Antikörper (Fluoreszenz-)konjugieren können und damit das Problem umgehen können, mit Antikörpern aus der Maus Mausschnitte anzufärben: www.abcam.com/EasyLink

      Sollten Sie sich für ein nicht aufgereinigtes Antiserum oder einen Antikörper entscheiden, dessen Puffer BSA oder dergleichen enthält, empfiehlt sich eine Aufreinigung vor der Konjugation. Für diese Zwecke haben wir wie angesprochen Purification Kits (z.B. ab102783 oder ab102784) erhältlich. Weitere Informationen dazu finden Sie unter https://www.abcam.com/index.html?pageconfig=resource&rid=13489 und https://www.abcam.com/index.html?pageconfig=resource&rid=13156.

      Die Möglichkeit, Antikörper mit Goldpartikeln zu konjugieren, bieten wir derzeit leider nicht an. Vielen Dank jedoch für die Anregung; die Kollegen in der Produktabteilung freuen sich sehr über dieses Feedback. Wir haben Gold-gelabelte Sekundärantikörper in unserem Katalog (1.4nm-20nm), und ich bin Ihnen gerne behilflich, einen für Sie geeigneten herauszusuchen, wenn Sie Interesse daran haben.

      Derzeit haben wir auch über 300 Antikörper in unserem Sortiment, die für die Detektion grampositiver Bakterien geeignet sind. Sie finden diese auf unserer Webseite unter:

      Primary antibodies (nur mit der Maus drüberfahren, nicht klicken) à Microbiology à Bacteria à Gram positive Bacteria

      oder hier: https://www.abcam.com/index.html?pageconfig=productmap&cl=252

      Alles, was wir über diese Antikörper wissen (also auch die Eignung für IHC an Paraffinschnitten), finden Sie auf den Datenblättern. Unter Umständen können Sie auch mit einem dieser Antikörper an unserem Testprogramm teilnehmen (https://www.abcam.com/collaborationdiscount).

      Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

      Ich wünsche Ihnen ein schönes Wochenende!

      Read More

      Abcam Scientific Support

      Answered on Mar 09 2012

      Immunohistochemistry (Frozen sections) abreview for Anti-GAP43 antibody - Neuronal Marker

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Xenopus laevis Tissue sections (Spinal cord (on sections of whole tadpole))
      Permeabilization
      Yes - 0.2% Triton-X/100
      Specification
      Spinal cord (on sections of whole tadpole)
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jan 06 2016

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GAP43 antibody - Neuronal Marker

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      Commercial Peroxidase blocking buffer as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Commercial High pH Buffer
      Sample
      Human Tissue sections (Tendon)
      Specification
      Tendon
      Permeabilization
      No
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 14 2014

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GAP43 antibody - Neuronal Marker

      Average
      Abreviews
      Abreviews
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (brain)
      Specification
      brain
      Fixative
      Paraformaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citrate
      Permeabilization
      No
      Blocking step
      Serum as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 5°C
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 08 2011

      Western blot abreview for Anti-GAP43 antibody - Neuronal Marker

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - other (brain)
      Loading amount
      10 µg
      Specification
      brain
      Gel Running Conditions
      Reduced Denaturing (12% SDS)
      Blocking step
      Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 10 2009

      Western blot abreview for Anti-GAP43 antibody - Neuronal Marker

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (Brain, Sciatic Nerve)
      Loading amount
      20 µg
      Specification
      Brain, Sciatic Nerve
      Blocking step
      Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
      Read More

      Abcam user community

      Verified customer

      Submitted Jul 04 2006

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