Anti-GAP43 antibody - Neuronal Marker (ab12274)
- Datasheet
- Specific References (13)
- Protocols
Overview
-
Product nameAnti-GAP43 antibody - Neuronal Marker
See all GAP43 primary antibodies -
DescriptionRabbit polyclonal to GAP43 - Neuronal Marker
-
Host speciesRabbit
-
Tested applicationsSuitable for: IHC-P, WB, ICC, IP, IHC-Frmore details
-
Species reactivityReacts with: Mouse, Rat, Cat, Human, Fish, Monkey
-
Immunogen
GAP43 purified from cat brain.
-
Positive control
- ICC/IF: SKNSH cells.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
Storage bufferPreservative: 0.01% Sodium Azide
-
PurityWhole antiserum
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab12274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | 1/500 - 1/2000. Detects a band of approximately 43-57 kDa (predicted molecular weight: 24 kDa). | |
| ICC | 1/100 - 1/400. | |
| IP | Use at an assay dependent concentration. | |
| IHC-Fr | 1/100 - 1/400. |
Target
-
FunctionThis protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons.
-
Sequence similaritiesBelongs to the neuromodulin family.
Contains 1 IQ domain. -
Post-translational
modificationsPhosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. -
Cellular localizationCell membrane. Cell projection > growth cone membrane. Cell junction > synapse. Cytoplasmic surface of growth cone and synaptic plasma membranes.
- Information by UniProt
-
Database links
- Entrez Gene: 2596 Human
- Entrez Gene: 14432 Mouse
- Entrez Gene: 29423 Rat
- GenBank: NP_002036 Human
- Omim: 162060 Human
- SwissProt: P17677 Human
- SwissProt: P06837 Mouse
- SwissProt: P07936 Rat
see all -
Alternative names
- Axonal membrane protein GAP 43 antibody
- Axonal membrane protein GAP-43 antibody
- B 50 antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-GAP43 antibody - Neuronal Marker (ab12274)ICC/IF image of ab12274 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12274, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
-
Western blot - Anti-GAP43 antibody - Neuronal Marker (ab12274)This image is courtesy of an anonymous AbreviewWestern blot showing detection of GAP43 in Mouse Whole brain tissue lysates using ab12274 (1/5000) in conjunction with a goat anti-rabbit secondary antibody conjugated to HRP (1/10000). Ab12274 did not detect GAP43 in Sciatic nerve lysates (negative control). Western blot showing detection of GAP43 in Mouse Whole brain tissue lysates using ab12274 (1/5000) in conjunction with a goat anti-rabbit secondary antibody conjugated to HRP (1/10000). Ab12274 did not detect GAP43 in Sciatic nerve lysates (negative control). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAP43 antibody - Neuronal Marker (ab12274)Ab12274 staining human neuropil. Staining is localized to the cytoplasm and membrane.
Left panel: with primary antibody diluted 1:2000. Right panel: isotype control.
Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, citrate pH 6.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Protocols
References
This product has been referenced in:
- Pohland M et al. MH84 improves mitochondrial dysfunction in a mouse model of early Alzheimer's disease. Alzheimers Res Ther 10:18 (2018). Read more (PubMed: 29433569) »
- Mohd Isa IL et al. Implantation of hyaluronic acid hydrogel prevents the pain phenotype in a rat model of intervertebral disc injury. Sci Adv 4:eaaq0597 (2018). Read more (PubMed: 29632893) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"