Anti-GAP43 antibody - Neuronal Marker (ab12274)
Key features and details
- Rabbit polyclonal to GAP43 - Neuronal Marker
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-GAP43 antibody - Neuronal Marker
See all GAP43 primary antibodies -
Description
Rabbit polyclonal to GAP43 - Neuronal Marker -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Cat GAP43.
Database link: Q6S9D9 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab12274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (2) |
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Notes |
|---|
|
IHC-P
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. -
Sequence similarities
Belongs to the neuromodulin family.
Contains 1 IQ domain. -
Post-translational
modificationsPhosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. -
Cellular localization
Cell membrane. Cell projection > growth cone membrane. Cell junction > synapse. Cytoplasmic surface of growth cone and synaptic plasma membranes. - Information by UniProt
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Database links
- Entrez Gene: 2596 Human
- GenBank: NP_002036 Human
- Omim: 162060 Human
- SwissProt: P17677 Human
- Unigene: 134974 Human
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Alternative names
- Axonal membrane protein GAP 43 antibody
- Axonal membrane protein GAP-43 antibody
- B 50 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAP43 antibody - Neuronal Marker (ab12274)Ab12274 staining human neuropil. Staining is localized to the cytoplasm and membrane.
Left panel: with primary antibody diluted 1:2000. Right panel: isotype control.
Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, citrate pH 6.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Protocols
Datasheets and documents
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Datasheet download
References (24)
ab12274 has been referenced in 24 publications.
- Li L et al. Proteomic Analysis of Rhesus Macaque Brain Explants Treated With Borrelia burgdorferi Identifies Host GAP-43 as a Potential Factor Associated With Lyme Neuroborreliosis. Front Cell Infect Microbiol 11:647662 (2021). PubMed: 34178719
- Mak HK et al. MicroRNA-19a-PTEN Axis Is Involved in the Developmental Decline of Axon Regenerative Capacity in Retinal Ganglion Cells. Mol Ther Nucleic Acids 21:251-263 (2020). PubMed: 32599451
- Rao F et al. Aligned chitosan nanofiber hydrogel grafted with peptides mimicking bioactive brain-derived neurotrophic factor and vascular endothelial growth factor repair long-distance sciatic nerve defects in rats. Theranostics 10:1590-1603 (2020). PubMed: 32042324
- Gu J et al. Neurobehavioral effects of bisphenol S exposure in early life stages of zebrafish larvae (Danio rerio). Chemosphere 217:629-635 (2019). PubMed: 30447611
- Hu T et al. Electro-acupuncture-induced neuroprotection is associated with activation of the IGF-1/PI3K/Akt pathway following adjacent dorsal root ganglionectomies in rats. Int J Mol Med 43:807-820 (2019). PubMed: 30569108
