Application
Western blot
Sample
Human Cell lysate - whole cell (U87 cells)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-20% gel)
Loading amount
15 µg
Treatment
bevacizumab 2mg/ml for 24hrs, 48hrs
Specification
U87 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
13 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA
Dilution
1/5000
Detection
Detection method
ECL
Exposure
5 second(s)
Bands
Specific: 37 kDa
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Submitted May 11 2020