Application
Western blot
Sample
Human Cell lysate - whole cell (Hela cells)
Gel Running Conditions
Non-reduced Denaturing (4-12% BIS-TRIS gradient, MOPS running buffer)
Loading amount
20 µg
Specification
Hela cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5%milk,1x TBS, antibody 1:10,000 dilute
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: Cell signal 7076s anti mouse
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
None (but doesn't react to MW markers)
Exposure
10 second(s)
Bands
Specific: 35-40 kDa
Positive control
Lysates
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Mar 22 2021