Product nameAnti-GAPDH antibody
See all GAPDH primary antibodies
DescriptionRabbit polyclonal to GAPDH
Broad reactivity makes this antibody an excellent loading control.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: a wide range of other species
Synthetic peptide corresponding to Human GAPDH (N terminal).
Database link: P04406
- WB: NIH/3T3, HEK-293, Molt-4, A549, Jurkat and HeLa lysate. HeLa nuclear lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 0.42% Potassium phosphate
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab263962 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa.|
FunctionHas both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Sequence similaritiesBelongs to the glyceraldehyde-3-phosphate dehydrogenase family.
modificationsS-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
Cellular localizationCytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
- Information by UniProt
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All lanes : Anti-GAPDH antibody (ab263962) at 1/1000 dilution (overnight at 4degC)
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) lysate
Lane 3 : Molt-4 (human lymphoblastic leukemia cell line) lysate
Lane 4 : A549 (human lung carcinoma cell line) lysate
Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) lysate
Lane 6 : NIH/3T3 nuclear lysate
Lane 7 : HeLa nuclear lysate
Lane 8 : Jurkat (human T cell leukemia cell line from peripheral blood) lysate
Lysates/proteins at 35 µg per lane.
All lanes : Peroxidase rabbit secondary antibody for 30 min at RT at 1/70000 dilution
Predicted band size: 36 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab263962 has not yet been referenced specifically in any publications.