Immunocytochemistry/ Immunofluorescence abreview for Anti-GAPDH antibody [EPR16891]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (undifferentiated Human ES cells on MEFs)
Permeabilization
Yes - see blocking buffer
Specification
undifferentiated Human ES cells on MEFs
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% serum, 0.1% triton, 0.1% BSA in PBS
Dilution
1/500

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: donkey anti rabbit IgG Alexa fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
Following fixation in 4% PFA, hESCs were assessed for GAPDH expression using 1:100 dilution of the primary antibody in 1% serum, 0.1% triton, 0.1% BSA in PBS followed by detection using a donkey anti goat IgG Alexa 488 (1:500). The results show positive expression in both MEFs and ES cells (green) and nuclei stained blue using DAPI.

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Submitted Feb 22 2016

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