Western blot abreview for Anti-GAPDH antibody [EPR16891]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (skin)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
skin
Blocking step
Commercial blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 23°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: blocking buffer
Dilution
1/5000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Goat anti rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680

Detection

Detection method
Odyssey fluorescent imaging
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: 37 kDa

Maral Tajerian

Verified customer

Submitted Jul 15 2016

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